Human chromosome banding specific for electron microscopy

Drouin, Régen; Messier, Paul-Emil; Richer, Claude‐Lise

doi:10.1159/000132526

Cited by 16 publications

(8 citation statements)

References 6 publications

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“…Indeed, after GB-AP banding, the BrdU-substituted C-band regions are often nega tively stained (Fig. 2), whereas after GB-AAu banding (Ta ble 1), the display of gold particles is different from that seen with euchromatic bands (Drouin et al, 1988b). The same Cband regions are GBG+.…”

Section: Replication Banding In Constitutive Helerochromatinmentioning

confidence: 94%

Complementary replication R- and G-band patterns induced by cell blocking at the R-band/G-band transition, a possible regulatory checkpoint within the S phase of the cell cycle

Fetni

Drouin²,

Richer³

et al. 1996

Cytogenet Genome Res

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The characteristic band patterns of replication banding (dynamic banding) were analyzed. High-resolution (550–1,250 bands per haploid genome) G- and R-band patterns were obtained after 5-bromo-2’-deoxyuridine (BrdU) incorporation during early or late S phase. Thymidine-BrdU permutation culture methods, which arrest DNA synthesis at the R-band/G-band transition, allow complementary BrdU substitution. The RBI (R bands by BrdU using immunological staining) and GBI (G bands by BrdU using immunological staining) band patterns were complementary for all chromosomes. There was no overlapping, and every part of each chromosome was positively stained by one of the two banding procedures. Cornparative analysis of RBG (R bands by BrdU using Giemsa staining) and RBI band patterns, as well as GBG (G bands by BrdU using Giemsa staining) and GBI band patterns, showed good congruency, displaying a very good band-for-band match. The congruency and complementarity found for these band patterns show that high concentrations of both thymidine and BrdU blocked S-phase progression near the R-band to G-band replication transition within the S phase. They also prove that BrdU incorporation is complementary and, therefore, demonstrate the existence of the R/G transition; a possible regulatory checkpoint within the S phase of the cell cycle.

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Section: Replication Banding In Constitutive Helerochromatinmentioning

confidence: 94%

Complementary replication R- and G-band patterns induced by cell blocking at the R-band/G-band transition, a possible regulatory checkpoint within the S phase of the cell cycle

Fetni

Drouin²,

Richer³

et al. 1996

Cytogenet Genome Res

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“…Electron microscopic examinations hold some promise for finer resolution of chromosomal abnormalities in the future (Drouin et al, 1988). Another advancing research tool is the use of molecular genetic techniques to detect structural mutations as small as a few kilobases in size.…”

Section: Background: Cytogenetic Techniquesmentioning

confidence: 99%

Chromosomal Aberrations and Schizophrenia

Bassett

1992

Br J Psychiatry

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Chromosomal aberrations associated with schizophrenic disorders may suggest regions in which to focus a search for genes predisposing to schizophrenia by a linkage strategy. As for other genetic illnesses, chromosomal abnormalities may also provide useful tools for subsequent physical mapping, fine localisation, and isolation of important susceptibility genes. Identification of several chromosomal aberrations may be especially important, given the unknown pathophysiology, the paucity of known brain genes, and the probable genetic heterogeneity of schizophrenia and manic-depression. However, because psychiatric disorders are common and inherited in a complex manner, researchers must use caution when drawing inferences about associations with chromosomal aberrations. Reported abnormalities involving autosomes (chromosomes 1 -22) associated with psychotic disorders are reviewed. Their relevance to linkage studies localising genes for schizophrenia was estimated by standardised criteria for specificity, diagnosis, family history, and overall weight of evidence. Four 'possibly relevant' chromosomal regions were identified: 5q, 11q, 18q, and 19p. This paper outlines strategies for future studies to detect new chromosomal aberrations associated with major psychotic disorders that may be relevant to isolating the genes for schizophrenia.

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“…To explain this differ ence. it is possible to conceive, for example, of stepwise replication of subband structures; however, such a phenomenon has not been observed with high-resolution replication banding (Drouin et al" 1988) or after very short pulse labeling (Vogel et al, 1989).…”

mentioning

confidence: 99%

“…Combining the BrdU antibody technique (BAT) with histone depletion might help to resolve replication band patterns. However, techniques designed to detect BrdU incorporation by electron microscopy (Drouin et al, 1988;Vogel et al, 1990) destroy histone-depleted chromosomes prepared by conventional methods. We report here on a modified technique for preparing histone-depleted chromosomes and on the demonstration of replication patterns with BAT.…”

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confidence: 99%

Demonstration of replication patterns on histone-depleted chromosomes

Just

Autenrieth²,

Vogel³

1993

Cytogenet Genome Res

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A modified immunologic technique is described for the purpose of demonstrating replication patterns on mammalian chromosomes after partial histone depletion. Replication patterns were induced by BrdU substitution and visualized by BrdU antibodies, coupled with peroxidase (diaminobenzidine/H₂O₂ or immunogold-detection. The replication patterns obtained by this technique did not reveal any additional details of replication compared to those shown by conventional cytogenetic staining. However, the possibility of demonstrating replication patterns on these partially histone-depleted chromosomes may prove useful for chromosomal in situ hybridization studies since the chromosomes produced are considerably larger than those seen in conventional preparations.

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Human chromosome banding specific for electron microscopy

Cited by 16 publications

References 6 publications

Complementary replication R- and G-band patterns induced by cell blocking at the R-band/G-band transition, a possible regulatory checkpoint within the S phase of the cell cycle

Complementary replication R- and G-band patterns induced by cell blocking at the R-band/G-band transition, a possible regulatory checkpoint within the S phase of the cell cycle

Chromosomal Aberrations and Schizophrenia

Demonstration of replication patterns on histone-depleted chromosomes

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