Human Chorionic Gonadotropin Secretion and Protein Phosphorylation in Chorionic Tissue.

Yamawaki, Takaharu; Toyoda, Nagayasu

doi:10.1507/endocrj.41.509

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…22 Treatment with PMA was also shown to induce the expression of differentiation markers. 23 Unstimulated BeWo were found to express low amounts of EBI3. Stimulation with forskolin and PMA, alone or in combination, resulted in clear upregulation of EBI3 expression after 48 hours of stimulation, which was maintained after 70 hours of stimulation, as shown by Western blotting.…”

Section: Ebi3 Expression Is Up-regulated During Differentiation In Chmentioning

confidence: 96%

Expression of Epstein-Barr Virus-Induced Gene 3, an Interleukin-12 p40-Related Molecule, throughout Human Pregnancy

Devergne

Coulomb-L’Herminé

Capel

et al. 2001

The American Journal of Pathology

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In human pregnancy, trophoblasts are the only cells of fetal origin in direct contact with the maternal immune system: syncytiotrophoblasts are in contact with maternal blood, whereas extravillous trophoblasts are in contact with numerous maternal uterine natural killer (NK) cells. Therefore, trophoblasts are thought to play a key role in maternal tolerance to the semiallogeneic fetus, in part through cytokine production and NK cell interaction. Epstein-Barr virus-induced gene 3 (EBI3) encodes a soluble hematopoietin receptor related to the p40 subunit of interleukin-12. Previous studies indicated that EBI3 is expressed in the spleen and tonsils, and at high levels in full-term placenta. To investigate further EBI3 expression throughout human pregnancy, we generated monoclonal antibodies specific for EBI3 and developed an EBI3 enzyme-linked immunosorbent assay. Immunohistochemical experiments with EBI3 monoclonal antibody on first-, second-, and third-trimester placental tissues demonstrated that EBI3 was expressed throughout pregnancy by syncytiotrophoblasts and extravillous trophoblasts (cytotrophoblast cell columns, interstitial trophoblasts, multinucleated giant cells, and trophoblasts of the chorion laeve). EBI3 expression was also induced during in vitro differentiation of trophoblast cell lines. In addition, large amounts of secreted EBI3 were detected in explant cultures from first-trimester and term placentae. Consistent with these data, EBI3 levels were strongly up-regulated in sera from pregnant women and gradually increased with gestational age. These data, together with the finding that EBI3 peptide is presented by HLA-G, suggest that EBI3 is an important immunomodulator in the fetal-maternal relationship, possibly involved in NK cell regulation.

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Section: Ebi3 Expression Is Up-regulated During Differentiation In Chmentioning

confidence: 96%

Expression of Epstein-Barr Virus-Induced Gene 3, an Interleukin-12 p40-Related Molecule, throughout Human Pregnancy

Devergne

Coulomb-L’Herminé

Capel

et al. 2001

The American Journal of Pathology

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Clinical and Biomedical Applications of Proteomics

Hochstrasser¹

1997

Proteome Research: New Frontiers in Functional Genomics

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Coincident expression of Menkes gene with copper efflux in human placental cells

Qian

Majumdar

Reddy

et al. 1996

American Journal of Physiology-Cell Physiology

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BeWo cells, a human choriocarcinoma cell line, have a high-affinity system for transporting copper ions into the cell (Km = 0.21 microM) but are sluggish in releasing copper back into the medium from preloaded cells. The slow efflux rate has recently been shown to correlate with a failure of BeWo cells to express the Menkes transcript [Y. Qian, E. Tiffany-Castiglioni, and E. D. Harris. Am. J. Physiol. 271 (Cell Physiol. 40). In press]. We have now determined that only when BeWo cells were grown on plastic surfaces such as petri dishes or flasks did they display negligible release and enhanced retention of 67Cu. Reverse transcriptase-polymerase chain reaction with the use of primers selective for the Menkes gene failed to show any evidence of a Menkes transcript in cells cultured on plastic surfaces. In contrast, cells grown on porous filters previously shown to allow apical and basolateral surfaces to develop did display the transcript and showed significant copper release with normal retention. Release of copper from filter-grown cells was blocked with p-chloromercuribenzoate, thus confirming sulfhydryl group involvement. Absorption of the 67Cu, either as a free ion or bound to ceruloplasmin, was unaffected by the different culture conditions. The data link the Menkes gene product with the ability of cells to release copper ions. They also suggest that the expression of the Menkes gene may be regulated by the development of polarized cell membranes.

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Human Chorionic Gonadotropin Secretion and Protein Phosphorylation in Chorionic Tissue.

Cited by 4 publications

References 24 publications

Expression of Epstein-Barr Virus-Induced Gene 3, an Interleukin-12 p40-Related Molecule, throughout Human Pregnancy

Expression of Epstein-Barr Virus-Induced Gene 3, an Interleukin-12 p40-Related Molecule, throughout Human Pregnancy

Clinical and Biomedical Applications of Proteomics

Coincident expression of Menkes gene with copper efflux in human placental cells

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