Human cell line (COLO 357) of metastatic pancreatic adenocarcinoma

Abstract

A continuous human cell line, COLO 357, with exceptional characteristics was derived from a metastasis of a pancreatic adenocarcinoma. COLO 357 grew as an adhering monolayer with a cell doubling time of 21 h and grew with 10% clonal efficiency in soft agar. COLO 357 cells had numerous lamellar inclusions. The cells elaborated the pancreatic enzymes trypsin, elastase and chymotrypsin. COLO 357 also secreted appreciable amounts of carcinoembryonic antigen and human chorionic gonadotropin. COLO 357 had a chromosome mode of 53 with 20 identifiable Giemsa-banded marker chromosomes. Nine nucleolar organizing regions were found by silver-stained metaphase preparations. COLO 357 has been "fingerprinted" for seven allelic isozymes. This cell line has been maintained in active culture for over 2 years, is preserved in a cell bank, and is available to other investigators.

The Geranylgeranyltransferase-I Inhibitor GGTI-298 Arrests Human Tumor Cells in G0/G1 and Induces p21WAF1/CIP1/SDI1 in a p53-independent Manner

“…Colo 357 was a kind gift from Channing Der (University of North Carolina) (35). Panc-1 and Colo 357 were grown in Dulbecco's modified Eagle's medium, SKBr3 and MDAMB-231 were grown in RPMI 1640 medium, A-549 was grown in F12K medium, and HT-1080 was grown in minimal essential medium.…”

Stat3 activation regulates the expression of vascular endothelial growth factor and human pancreatic cancer angiogenesis and metastasis

“…The following human pancreatic adenocarcinoma cell lines were used in this study: Bxpc-3, CaPan-2, HPAF-II, HS766T, -1, and SW-1990, which were purchased from the American Type Culture Collection (Manassas, VA, USA); MDA Panc-3, which was a gift from Dr Paul J Chiao; and COLO 357, which was originally established from a pancreatic adenocarcinoma metastasis by Morgan et al (1980), and its fast-growing (FG) and liver metastatic variant (L3.3) in nude mice, which were established by Vezeridis et al (1990). All of the cell lines were maintained in plastic flasks as adherent monolayers in minimal essential medium (MEM) supplemented with 15% fetal bovine serum, sodium pyruvate, nonessential amino acids, l-glutamine, and a vitamin solution (Flow Laboratories, Rockville, MD, USA).…”

Analysis of gene expression in cancer cell lines identifies candidate markers for pancreatic tumorigenesis and metastasis

“…Nineteen pancreatic cancer cell lines were analyzed in this study: A818-4, 4 AsPC-1, 5 Capan-2, 6 CFPAC-1, 7 Colo-357, 8 FA6, 9 HPAF-II, 10 Hs766T, 11 IMIM-PC-2, 12 MDAPanc-3, 13 MiaPaCa-2, 14 PaCa-3, 15 Panc-1, 16 PaTu-I, PaTu-II, 17 PT45, 18 RWP, 19 SUIT-2 20 and T3M4. 21 These cell lines were obtained from Cancer Research U.K. cell production services (London Research Institute Clare Hall Laboratories, Potters Bar, U.K.) and cultured in E4 medium supplemented with 10% heat-inactivated fetal calf serum (Gibco-Life Technologies, Paisley, U.K.) until harvest when they reached 80% confluence.…”