Human Cell Encapsulation in Gel Microbeads with Cosynthesized Concentric Nanoporous Solid Shells

Zhou, Xiamo; Haraldsson, Tommy; Nania, Salvatore; Ribet, Federico; Palano, Giorgia; Heuchel, Rainer; Löhr, Matthias; Wijngaart, Wouter van der

doi:10.1002/adfm.201707129

Cited by 12 publications

(4 citation statements)

References 98 publications

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“…Cells entrapment techniques include physical encapsulation in polymeric beads, such as microgels ( Zhou et al, 2018 ; Veernala et al, 2021 ) or alginate beads ( Shao et al, 2020 ; Hasturk et al, 2022 ), penetration and attachment of cells into porous 3D scaffolds ( Wu et al, 2020 ; Czosseck et al, 2022 ) or fiber‐based matrices ( Matera et al, 2019 ; Davidson et al, 2021 ; Sahu et al, 2021 ), bioreactors based on porous membranes ( Skrzypek et al, 2017 ; Bose et al, 2020 ), films made of super‐adhesive materials ( Suneetha et al, 2019 ; Nagano et al, 2021 ) and antibody‐conjugated magnetic beads ( Xu H et al, 2011 ; Nath et al, 2015 ). These devices can be obtained using innovative technologies such as 3D printing ( Agarwal et al, 2020 ; Dey and Ozbolat, 2020 ), photolithography ( Tricinci et al, 2015 ; Larramendy et al, 2019 ; Tenje et al, 2020 ), electrospinning ( Canbolat et al, 2011 ; Zussman, 2011 ; Ang et al, 2014 ), emulsion methods to obtain polymeric droplets ( López et al, 1997 ; Chaemsawang et al, 2018 ; Qu et al, 2021 ), surface coating technologies ( Yoo et al, 2011 ), sol‐gel encapsulation ( Kamanina et al, 2022 ), template‐assisted techniques ( Khademhosseini et al, 2006 ), etc.…”

Section: Introductionmentioning

confidence: 99%

“…These devices can be obtained using innovative technologies such as 3D printing ( Agarwal et al, 2020 ; Dey and Ozbolat, 2020 ), photolithography ( Tricinci et al, 2015 ; Larramendy et al, 2019 ; Tenje et al, 2020 ), electrospinning ( Canbolat et al, 2011 ; Zussman, 2011 ; Ang et al, 2014 ), emulsion methods to obtain polymeric droplets ( López et al, 1997 ; Chaemsawang et al, 2018 ; Qu et al, 2021 ), surface coating technologies ( Yoo et al, 2011 ), sol‐gel encapsulation ( Kamanina et al, 2022 ), template‐assisted techniques ( Khademhosseini et al, 2006 ), etc. The cells are kept inside the device through physical immobilization ( Zhou et al, 2018 ; Shao et al, 2020 ; Veernala et al, 2021 ; Hasturk et al, 2022 ), extracellular‐matrix‐like adherence ( Rao and Winter 2009 ), specific antigen‐antibody recognition ( Roupioz et al, 2011 ; Boulanger et al, 2022 ), barrier containing ( Spagnolo et al, 2015 ; Larramendy et al, 2019 ; Li et al, 2021b ) and external stimuli‐activated entrapment ( Fu et al, 2008 ; Long et al, 2020 ), etc. The entrapment of cells can be switched on and off through enzymatic control ( Chen et al, 2003 ), pH variations ( Kocak et al, 2017 ), barrier containing ( Seifan et al, 2017 ; Larramendy et al, 2019 ; Gatto et al, 2023 ) or radiation switch ( Fu et al, 2008 ; Long et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

Popescu,

Calin,

Tanasa

et al. 2023

Front. Bioeng. Biotechnol.

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The manipulation of biological materials at cellular level constitutes a sine qua non and provocative research area regarding the development of micro/nano‐medicine. In this study, we report on 3D superparamagnetic microcage‐like structures that, in conjunction with an externally applied static magnetic field, were highly efficient in entrapping cells. The microcage‐like structures were fabricated using Laser Direct Writing via Two‐Photon Polymerization (LDW via TPP) of IP‐L780 biocompatible photopolymer/iron oxide superparamagnetic nanoparticles (MNPs) composite. The unique properties of LDW via TPP technique enabled the reproduction of the complex architecture of the 3D structures, with a very high accuracy i.e., about 90 nm lateral resolution. 3D hyperspectral microscopy was employed to investigate the structural and compositional characteristics of the microcage‐like structures. Scanning Electron Microscopy coupled with Energy Dispersive X‐Ray Spectroscopy was used to prove the unique features regarding the morphology and the functionality of the 3D structures seeded with MG‐63 osteoblast‐like cells. Comparative studies were made on microcage‐like structures made of IP‐L780 photopolymer alone (i.e., without superparamagnetic properties). We found that the cell‐seeded structures made by IP‐L780/MNPs composite actuated by static magnetic fields of 1.3 T were 13.66 ± 5.11 folds (p < 0.01) more efficient in terms of cells entrapment than the structures made by IP‐L780 photopolymer alone (i.e., that could not be actuated magnetically). The unique 3D architecture of the microcage‐like superparamagnetic structures and their actuation by external static magnetic fields acted in synergy for entrapping osteoblast‐like cells, showing a significant potential for bone tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

Popescu,

Calin,

Tanasa

et al. 2023

Front. Bioeng. Biotechnol.

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“…The cell encapsulation technology is promising for developing functional carriers to effectively protect cells from a host immune system upon transplantation, thus highly potential for reducing the requirement of an immunosuppressant. − Accordingly, a myriad of functional carriers have been fabricated for the encapsulation applications in biomedical fields and generally categorized into two types based on their size scale: micro- (<1 mm) and macrocarriers (>1 mm). − With well-established techniques such as microfluidics and electrojetting, functional carriers with microscale size are developed in the forms of microspheres and microfibers for cell encapsulation. − Using a more biocompatible all-in-water system during the microfluidic fabrication process, microcapsules containing an aqueous core shielded by a hydrogel shell can be generated for more efficient encapsulation. − Due to the inherent structural feature, microcarriers exhibit a large surface area to volume ratio, which facilitates the mass transfer for maintaining the viability and functions of the loaded cells. Generally, hiPSC-derived organoids are featured with size of hundreds of micrometers.…”

Section: Introductionmentioning

confidence: 99%

Controllable Fabrication of Composite Core–Shell Capsules at a Macroscale as Organoid Biocarriers

Tao

Liu

et al. 2021

ACS Appl. Bio Mater.

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The cell encapsulation technology is promising for generation of functional carriers with well-tailored structures for efficient transplantation and immunoprotection of cells/tissues. Stem cell organoids are highly potential for recapitulating the intricate architectures and functionalities of native organs and also providing an unlimited cell source for cellular replacement therapy. However, it remains challenging for loading the organoids with hundreds of micrometers size by current existing cell carriers. Herein, a simple and facile coextrusion strategy is developed for controllable fabrication of Ca-alginate/poly(ethylene imine) (Alg/ PEI) macrocapsules for efficient encapsulation and cultivation of organoids. Human-induced pluripotent stem cell (hiPSC)-derived islet organoids are encapsulated in the aqueous compartments of the capsules and immunoisolated by a semipermeable Alg/PEI shell. Via electrostatic interactions, a PEI polyelectrolyte can be incorporated in the shell for restricting its swelling, thus effectively improving the stability of the capsules. The Alg/PEI macrocapsules are featured with desirable selective permeability for immunoisolation of antibodies from reaching the loaded organoids. Meanwhile, they also exhibit excellent permeability for mass transfer due to their well-defined core−shell structure. As such, the encapsulated islet organoids contain islet-specific multicellular components, with high viability and sensitive glucosestimulated insulin secretion function. The proposed approach provides a versatile encapsulation system for tissue engineering and regenerative medicine applications.

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“…Micro- and nanocapsules pose immense advantages in a number of applications, ranging from biology and medicine to environmental and industrial applications, due to the controlled release and isolation of the encapsulated molecules from a potentially harmful external environment . Capsules offer several benefits over films, for example, the possibility of multicompartmentalization and liquid mobility, improved protection of the encapsulated molecules, and enhanced flexibility in terms of composition and functionality; capsules can be embedded in various matrices, for example, printable inks that adhere to different surfaces. − From a materials point of view, various types of molecules and nanoparticles, such as plasmonic or photoluminescent nanoparticles and biological compounds, can be encapsulated. ,− …”

Section: Introductionmentioning

confidence: 99%

Porous Cellulose Nanofiber-Based Microcapsules for Biomolecular Sensing

Paulraj

Wennmalm

Riazanova

et al. 2018

ACS Appl. Mater. Interfaces

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Cellulose nanofibers (CNFs) have recently attracted a lot of attention in sensing because of their multifunctional character and properties such as renewability, nontoxicity, biodegradability, printability, and optical transparency in addition to unique physicochemical, barrier, and mechanical properties. However, the focus has exclusively been devoted toward developing two-dimensional sensing platforms in the form of nanopaper or nanocellulose-based hydrogels. To improve the flexibility and sensing performance in situ, for example, to detect biomarkers in vivo for early disease diagnostics, more advanced CNF-based structures are needed. Here, we developed porous and hollow, yet robust, CNF-based microcapsules using only the primary plant cell wall components, CNF, pectin, and xyloglucan, to assemble the capsule wall. The fluorescein isothiocyanate-labeled dextrans with M W of 70 and 2000 kDa could enter the hollow capsules at a rate of 0.13 ± 0.04 and 0.014 ± 0.009 s–1, respectively. This property is very attractive because it minimizes the influence of mass transport through the capsule wall on the response time. As a proof of concept, glucose oxidase (GOx) enzyme was loaded (and cross-linked) in the microcapsule interior with an encapsulation efficiency of 68 ± 2%. The GOx-loaded microcapsules were immobilized on a variety of surfaces (here, inside a flow channel, on a carbon-coated sensor or a graphite rod) and glucose concentrations up to 10 mM could successfully be measured. The present concept offers new opportunities in the development of simple, more efficient, and disposable nanocellulose-based analytical devices for several sensing applications including environmental monitoring, healthcare, and diagnostics.

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Human Cell Encapsulation in Gel Microbeads with Cosynthesized Concentric Nanoporous Solid Shells

Abstract: Cover: Confocal image of primary human cardiac fibroblasts in culture. Focus on Fibroblasts: Development, plasticity, and therapeutic challenges in the cardiac fibroblast lineage THESIS FOR DOCTORAL DEGREE (Ph.D.

Cited by 12 publications

References 98 publications

Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

Magnetically‐actuated microcages for cells entrapment, fabricated by laser direct writing via two photon polymerization

Controllable Fabrication of Composite Core–Shell Capsules at a Macroscale as Organoid Biocarriers

Porous Cellulose Nanofiber-Based Microcapsules for Biomolecular Sensing

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