Human CD34+ cells isolated from bone marrow peripheral blood, cord blood and thymus secrete CCR5 binding β-chemokines

Majka, Marcin; Rozmysłowicz, Tomasz; Pietrzkowski, Zbigniew; Ratajczak, Janina; Dobrowsky, Adrian; Janowska‐Wieczorek, Anna; Ratajczak, Mariusz Z.

doi:10.1016/s0301-472x(00)00357-x

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2018

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“…Beyond the extracellular matrix features of the niche, HSCs are surrounded by cellular components within the bone marrow. Niche-associated cells can exert an influence on HSC activity via cadherin interactions, as well as secreted biomolecular factors [20,21]. In the native niche, the structure of the ECM modulates biotransport of secreted factors via presentation of transient binding motifs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stromal cell remodeling of a gelatin hydrogel microenvironment defines an artificial hematopoietic stem cell niche

Gilchrist

Lee

et al. 2018

Preprint

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We identify a combination of an initially low-diffusivity (autocrine dominated) hydrogel and a 1:1 HSCP:MSC seeding ratio as conducive to enhanced HSC population maintenance. Further, gene expression and serial mechanical testing data suggests that MSC-mediated matrix remodeling is significant for the long-term HSC culture, reducing HSC autocrine feedback and potentially enhancing MSC-mediated paracrine signaling over 7-day culture in vitro. This work demonstrates the design of an HSC culture system that couples initial hydrogel properties, MSC co-culture, and concepts of dynamic reciprocity mediated by MSC remodeling to achieve enhanced HSC maintenance.

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Section: Introductionmentioning

confidence: 99%