Human Cartilage Grafts Stored in Air

Culture and Cryopreservation of Chondrocytes from Human Cartilage: Relevance for Cartilage Allografting in Otolaryngology

“…Type II DNase was used to disperse DNA released from lysed cells as it tends to impair proteolysis and promote reaggre gation [4], Viable cartilage cells were isolated not only from speci mens obtained from patients during reconstructive sur gery but also from cadavers some hours after death. Hagerty et al [5] showed that at least 95% of the cells of costal cartilage are still viable 6 h after the death of the individual. Although the extraction from cadavers could not be performed under sterile conditions, bacterial con tamination could be avoided by the use of noncytotoxic dosages of gentamycin and amphotericin B.…”

The Use of Preserved Autogenous Septal Cartilage in "Touch-up" Rhinoplasty

“…7 The autogenous preserved cartilage has been well described for use in both aesthetic and reconstructive rhinoplasty surgery for well over 50 years (see reviews by Donald, 8 Sailer, 9 and Sarnat and Laskin 10 ). Many methods to preserve autogenous cartilage have been used, including refrigeration or freezing (typically at 0°-4°C) in saline 11,12 and plasma, 13 boiling, 14 preservation in merthiolate [15][16][17][18][19] or 2-ethylmercurithiobenzoxazole-5-carboxylic acid (Cialit), 20 immersion in ethanol or isopropyl alcohol, 21 storage in mecurochrome (L. Bernstein, DDS, MD, e-mail communication, February 2000), preservation in polyester resins, 22 and lyophilization. 9 Few systematic studies on the long-term behavior of preserved grafts exist, although a survey by Donald and Col 23 attempted to poll practice patterns.…”

“…9,13,[15][16][17]21,[25][26][27][28][29][30][31][32][33] Several studies have documented the long-term maintenance of graft integrity, including a remarkable study by Muhlbauer et al 17 documenting the replacement of merthiolatepreserved homologous rib cartilage onlay grafts to the dorsum with cancellous bone matrix. Ossification, as opposed to the simple deposition of calcium in the implant, is thought to occur when grafts are in direct contact with adjacent periosteal tissues.…”

Immunologie vitaler und konservierter Transplantate

“…Ein wichtiges zusatzliches Problem ergibt sich aus der Tatsache, daB die Konservierung zu einer Devitalisierung des Gewebes fiihrt [101,102,128,156]. Einige der Resorptionsphanomene werden auf eine schlechte Konservierung des Transplantats zuriickgefiihrt.…”