Human Cancer and Primary Cell Culture in the New Hybrid Bioreactor System Tecnomouse

Nagel, Angela; Effenberger, E.; Koch, Stefan; Marx, Uwe; Lubbe, Lizelle

doi:10.1016/b978-0-7506-1845-8.50072-7

Cited by 7 publications

(5 citation statements)

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“…Hollow-fiber systems are widely used in the production of biopharmaceuticals including monoclonal antibodies. Several examples of modified membrane bioreactors exist for the 3D culture of tissue cells including hepatocytes (4, 50-54), skin cells (55) or other human cells (49,56).…”

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

Bioreactor design for tissue engineering

Pörtner

Nagel-Heyer

Goepfert

et al. 2005

Journal of Bioscience and Bioengineering

276

174

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Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

Bioreactor design for tissue engineering

Pörtner

Nagel-Heyer

Goepfert

et al. 2005

Journal of Bioscience and Bioengineering

276

174

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“…In membrane bioreactors, including hollow-fi ber reactors [56], the miniPerm system [57] or the tecnomouse [58], cells are cultivated at tissue-like densities in a compartment which contains one or several types of membrane for nutrient and oxygen supply and removal of toxic metabolites. Hollow-fi ber systems are widely used in the production of biopharmaceuticals, including monoclonal antibodies.…”

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

“…Hollow-fi ber systems are widely used in the production of biopharmaceuticals, including monoclonal antibodies. Several examples of modifi ed membrane bioreactors exist for the 3D culture of tissue cells, including hepatocytes [6,[59][60][61][62][63][64], skin cells [65] or other human cells [58,66]. Most of the culture systems and bioreactors discussed so far were fi rst developed for the cultivation of mammalian cells, and subsequently adapted to the engineering of 3D tissue constructs.…”

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

“…These types of reactor are very effi cient for the long-term cultivation of mammalian cells to produce biopharmaceuticals, such as monoclonal antibodies, recombinant drugs including tissue plasminogen activator (tPA) and erythropoietin (EPO), or recombinant retroviruses for gene therapy [48][49][50]. For tissue engineering, the reactors have been investigated for several applications, including the cultivation of "liver" cells as an extracorporeal liver device [6,18,51] In membrane bioreactors, including hollow-fi ber reactors [56], the miniPerm system [57] or the tecnomouse [58], cells are cultivated at tissue-like densities in a compartment which contains one or several types of membrane for nutrient and oxygen supply and removal of toxic metabolites. Hollow-fi ber systems are widely used in the production of biopharmaceuticals, including monoclonal antibodies.…”

Section: Culture Systems and Bioreactors Used In Tissue Engineeringmentioning

confidence: 99%

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An Overview on Bioreactor Design, Prototyping and Process Control for Reproducible Three‐Dimensional Tissue Culture

Pörtner

Giese²

2006

Drug Testing in Vitro

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Bioreactor systems play an important role in tissue engineering, as they enable reproducible and controlled changes to be made in specifi c environmental factors. They can also provide the technical means to perform controlled studies aimed at understanding specifi c biological, chemical, or physical effects. Furthermore, bioreactors allow for a safe and reproducible production of tissue constructs. For later clinical applications, the bioreactor system should be an advantageous method in terms of low contamination risk, ease of handling, and scalability. With respect to drug screening, the main challenge is the effi cient, reproducible handling of a large quantity of tissue constructs in parallel (high-throughput screening). To date, the goals and expectations of bioreactor development have been fulfi lled only to some extent, as bioreactor design in tissue engineering is very complex and still at an early stage of development, especially for use in drug screening. In this chapter, important aspects of bioreactor design are summarized, and an overview of existing concepts is provided. An artifi cial immunosystem will be used as an example to demonstrate how an increased fundamental understanding of biological, biochemical, and engineering aspects can signifi cantly improve the properties of 3D tissue constructs.

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“…Productivity of the cell lines was also assessed at very high cell density in the Tecnomouse Hollow Fibre System (Nagel et al, 1994). As shown in Figure 3, this system allows direct aeration of the cells across the silicone membrane of the reactor, and thereby separates the aeration from the medium supply.…”

Section: Cell Cultivation In the Hollow Fibre Reactormentioning

confidence: 99%

Untitled

et al. 1999

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Apoptosis is an active, genetically determined death mechanism which can be induced by a wide range of physiological factors and by mild stress. It is the predominant form of cell death during the production of antibodies from murine hybridoma cell lines. A number of studies have now demonstrated that the suppression of this death pathway, by means of over-expression of survival genes such as bcl-2, results in improved cellular robustness and antibody productivity during batch culture. In the present study, the influence of bcl-2 expression on hybridoma productivity in two high density perfusion bioreactor systems was investigated. In the first system, a fixed-bed reactor, the DNA content in the spent medium was 25% higher in the control (TB/C3-pEF) culture than that found in the bcl-2 transfected (TB/C3-bcl2) cultures at all perfusion rates. This is indicative of a higher level of cell death in the control cell line. The average antibody concentration for the TB/C3-pEF cell line was 14.9 mg L-1 at perfusion rates of 2.6 and 5.2 d-1. However, for the TB/C3-bcl2 cell line it was 33 mg L-1 at dilution rates of 2 and 4 d-1. A substantial increase in antibody concentration was also found in the Integra Tecnomouse hollow fibre reactor. The antibody titre in the TB/C3-bcl2 cassette was nearly 100% higher than that in the TB/C3-pEF cassette during the cultivation period which lasted 6 weeks. Clearly, these results demonstrate the positive impact of bcl-2 over-expression on production of antibody in hybridoma perfusion cultures.

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Human Cancer and Primary Cell Culture in the New Hybrid Bioreactor System Tecnomouse

Cited by 7 publications

References 1 publication

Bioreactor design for tissue engineering

Bioreactor design for tissue engineering

An Overview on Bioreactor Design, Prototyping and Process Control for Reproducible Three‐Dimensional Tissue Culture

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