Human calcitonin has the same inhibitory effect on osteoclastic bone resorption by human giant cell tumor cells as salmon calcitonin

Abstract

The interface of bone marrow and trabeeular endostal surface is realised by cells of bone turnover, blood vessels, bone marrow cells,adipocytes or fibroblasts. With the bone marrow agelng the extent of adipocytes increases. We have observed histomorphometricaly a varying peritrabecular concentration of adipoeytee in cases of osteoporosis. It was to prove histomorphometrically the relationship between the percentage of the adipocytes occupied endostal bone surface (AS) and the histomorphometrical signs of bone turnover. Material and methods: undecalcified sections from 238 bone bioptates of metabolic bone diseases were quantitatively analysed. Bone volume(BY), osteoid ca) and eroded surface(Eel, oseoblasts(ObS) and osteoclasts occupied surface(OcS), total labelled surface (LS) and mineral apposition ratelMAR) were determined.In addition, the percentage of the trabecular bone surface with a contact to adipocytes(AS) was estimated. Resul~s:The adipocytes endost interface ranges from 0 to 100%, in the mean of the whole group 46%18. Negative correlations were detectabl~ between AS and the histomorphometric parameters of ES, OcS and ObS. In the subgroups of osteoporosis and of renal osteodystrophy(ROD) the results were different. In the osteoporosis (n=184) were detectable a mean AS of 50% and significant correlations between AS and ES, OcSObS but a low correlatiQn rate of AS to BV and to MAR. In ROD (n=29) We could detect a mean AS Of 31% and a significant correlation of AS to ES, OcS and ObS. Conclusions: A low cellular bone turnover activity is combined with a high percentage of adipecytes endost interface.In osteoporosis we found the highest percentage of adipocytes endost interface. In ROD seems to be possible by this way to determine and to quantify the process of adynamic renal bone disease. The change of osteoblasts into adipocytes could be a cause of the increasing AS.

Quantitative and reliable in vitro method combining scanning electron microscopy and image analysis for the screening of osteotropic modulators

“…Indeed, these should provide valuable information and eliminate, at least in part, possible discrepancies in results obtained from different studies (Cornish et al, 2001;Cudd et al, 1995;David et al, 1998;Iida et al, 1996;McSheehy et al, 1995).…”

“…They all come up against major limitations, including the small number of mature osteoclasts and low level of resorption leading to considerable variations in and between assays (Cornish et al, 2001;Cudd et al, 1995;David et al, 1998;Iida et al, 1996;McSheehy et al, 1995;Tamura et al, 1993a). …”