Human Breast Tumor Cells Express Multimodal Imaging Reporter Genes

Lin, Keh Ming; Hsu, Charles Ching–Hsiang; Chang, Wun-Shaing Wayne; Chen, Chiung‐Tong; Lee, Te‐Wei; Chen, Chin-Tu

doi:10.1007/s11307-008-0147-2

Cited by 11 publications

(13 citation statements)

References 30 publications

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“…While the first descriptions for this reporter construct were prior to the 2005 cutoff for this review,299,300 they are still actively employed in ongoing investigations particularly for labeling and observation of tumor cells,301,302 and they have been used to monitor promoter activity 303. However, fusion reporters are not without their limitations: it is quite common for a subunit in a fusion product to be inferior in performance to the freestanding protein.…”

Section: Genetic Programmingmentioning

confidence: 99%

Multimodality Imaging Probes: Design and Challenges

2010

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Her research focuses on the development of multimodal probes for molecular imaging. Recent work from her lab describes paramagnetic quantum dots, dualmode agents to visualize atherosclerosis, light-and redox-activatable MRI agents, and nontoxic silicon nanoparticles for MR/optical imaging. Dr. Louie is a member of Tau Beta Pi, the International Society for Magnetic Resonance in Medicine, the Biomedical Engineering Society, the Society of Women Engineers, and the Society for Molecular Imaging.

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Section: Genetic Programmingmentioning

confidence: 99%

Multimodality Imaging Probes: Design and Challenges

2010

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“…Imaging of transcriptional regulation of endogenous genes provides information on the many cellular processes associated with cancer [73], allowing the determination of gene profiles of different tumours [10,74]. This helps to improve the diagnosis of different types of tumours such as breast cancer [36,43].…”

Section: Gene Expression Studiesmentioning

confidence: 99%

Use of radionuclides in cancer research and treatment

Macias

2009

Clin Transl Oncol

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Cancer occurs as a result of misregulation of cell growth, which appears to be a consequence of alteration in the function of oncogenes and tumour suppressor genes. Ionising radiation has been used, since the discovery of X-rays in 1896 by Roentgen, both in cancer research and treatment of the disease. The main purpose of cancer research is to understand the molecular alterations involved in the development and progression of the disease in order to improve diagnosis and develop personalised therapies, by focusing on the features of the tumoral cell and the biological events associated to carcinogenesis. Radioisotopic techniques have been used routinely for in vitro research in the molecular and cellular biology of cancer for more than 20 years and are in the process of being substituted by alternative non-radioactive techniques. However in vivo techniques such as irradiation of cells in culture and/or experimental animal models and radioactive labelling are in development, due in part to advances in molecular imaging technologies. The objective of this review is to analyse in an integrative way the applications of ionising radiation in cancer research and therapy. It had been divided into two parts. The first one will approach the techniques applied to cancer research and the second will summarise how ionising radiation is applied to the treatment of neoplastic disease.

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“…PET/CT or SPECT/CT systems could both be utilized to image the HSV-tk metabolite (Figure 4a). 47 In other example, Kang et al has described a trimodality approach for sentinel lymphocyte detection, whereby OI was utilized for initial detection of signal, after which the signal from PET imaging was coregistered with both MRI (Figure 4e) and CT (Figure 4b) images. 48…”

Section: Multimodal Fusion Images and Hybrid Technologymentioning

confidence: 99%

“…Examples of multimodality images. (a) [ 123 I] FIAU SPECT and CT fusion image,47 (b,e) PET signal (localized color) overlaid on (b) CT image and (e) MR image,48 (c) Fused optical and CT images produced in the same bimodality instrument,46 (d) Optical image of mouse kidneys coregistered with an MR image 45. Images are adapted and reprinted with permission from the publisher or author(s).…”

mentioning

confidence: 99%

A Shift From Cell Cultures to Creatures: In Vivo Imaging of Small Animals in Experimental Regenerative Medicine

Studwell¹,

Kotton

2011

Molecular Therapy

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Although the use of small animals for in vivo experimentation has been widespread, only recently has there been easy availability of techniques that allow noninvasive in vivo imaging of small animals. Because these techniques allow the same individual subject to be followed longitudinally throughout the duration of an experiment, their use is rapidly changing the way small animals are employed in the laboratory. In this review, we focus on six imaging modalities that are increasingly employed for small animal in vivo imaging: optical imaging (OI), magnetic resonance imaging (MRI), computed tomography (CT), single-photon emission tomography (SPECT), ultrasound (US), and positron-emission tomography (PET). Each modality allows for the noninvasive tracking of cells and cell products in vivo. In addition, multimodality imaging, combining two or more of these techniques, has also been increasingly employed to overcome the limitations of each independent technique. After reviewing these available imaging modalities, we detail their experimental application, exemplified by the emerging field of regenerative medicine, referring to publications whose conclusions would otherwise be difficult to support without the availability of in vivo imaging.

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Human Breast Tumor Cells Express Multimodal Imaging Reporter Genes

Abstract: This study confirmed the usefulness of eGFP-tk in many applications by providing, in vitro and in vivo, the sensitive and reporter gene-specific imaging. ZR75-1-eGFP-tk cells that are ready to incorporate in various imaging platforms constitute a useful model in breast cancer research.

Cited by 11 publications

References 30 publications

Multimodality Imaging Probes: Design and Challenges

Multimodality Imaging Probes: Design and Challenges

Use of radionuclides in cancer research and treatment

A Shift From Cell Cultures to Creatures: In Vivo Imaging of Small Animals in Experimental Regenerative Medicine

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