Human breast milk T lymphocytes display the phenotype and functional characteristics of memory T cells

Bertotto, A; Gerli, Roberto; Fabietti, G.; Crupi, S.; Arcangeli, C; Scalise, F; Vaccaro, R

doi:10.1002/eji.1830200838

Cited by 96 publications

(45 citation statements)

References 27 publications

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“…Furthermore, this virus is transmitted via breast-feeding or sexual transmission through infected T cells, so infected T cells must enter breast milk or semen. Most T cells in breast milk are peripheral T cells with an effector/ memory phenotype (35). Restriction by TCF1/LEF1 would explain viral tropism to peripheral T cells and facilitate transmission of the virus.…”

Section: Discussionmentioning

confidence: 99%

TCF1 and LEF1 act as T-cell intrinsic HTLV-1 antagonists by targeting Tax

Ma¹,

Yasunaga²,

Akari³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

TCF1 and LEF1 act as T-cell intrinsic HTLV-1 antagonists by targeting Tax

Ma¹,

Yasunaga²,

Akari³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…PMN, which represent 5-25% of total cells, phagocytose and destroy any invading pathogens. The presence of lymphocytes in mammary secretions has also been reported; they contribute to the immune defences of new-borns in human and porcine species [4,27,68]. In goats, PMN constitute the major cell component among leukocytes.…”

Section: Cell Type Distribution In Milkmentioning

confidence: 97%

Potential uses of milk epithelial cells: a review

2002

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-Secretions collected from the mammary gland of different species contain heterogeneous populations of cells including lymphocytes, neutrophils, macrophages and epithelial cells in different species. Several factors influence the somatic cell count in milk and the distribution of cell types, such as species, infection status, physiological status and management practices. The epithelial cells are shed into milk during the lactation process. Most of them are viable and exhibit the characteristics of fully differentiated alveolar cells. Primary cultures of epithelial cells from colostrum and milk of humans, baboons, cows and goats together with established cell lines from human and goat milk, provide a good model for the study of lactogenesis, immunity transmission, cancer research and infection by viruses. The RNA extracted from milk cells have been shown to be representative of gene expression in the mammary gland and thus provide a source of material for molecular studies of gene expression and environmental interactions. milk / somatic cell count / epithelial cellsRésumé -Utilisations potentielles des cellules épithéliales du lait. Les sécrétions de la glande mammaire de différentes espèces, contiennent une population hétérogène de cellules incluant des cellules du système immunitaire et des cellules épithéliales. Divers facteurs influencent le nombre de cellules somatiques dans le lait et la distribution de chaque type cellulaire, en particulier l'espèce, le stade physiologique, l'état sanitaire et les conduites d'élevage. Les cellules épithéliales se détachent de l'épi-thélium mammaire au cours du processus de sécrétion du lait. Cependant, une grande majorité d'entre elles sont viables et présentent des caractéristiques de cellules alvéolaires totalement différenciées. En culture primaire ou en lignées cellulaires, elles constituent un modèle d'étude de l'action hormonale, de marqueurs de la cancérogenèse, de la transmission de l'immunité et d'infections virales. Les ARN totaux extraits des cellules du lait constituent une source de matériel représentatif de l'expression des gènes dans la glande mammaire et devraient permettre des études globales d'expression des gènes en interaction avec l'environnement de l'animal. lait / numération cellulaire / cellule épithéliale Reprod. Nutr. Dev. 42 (2002) 133-147 133

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“…However, antigen-induced proliferation strongly depends on the antigen used (Smith and Goldman, 1968;Parmely et al, 1977;Archambault et al, 1988b). Various reasons can be proposed for the lower proliferation of MGL than PBMC: fewer CD4 + cells producing growth promoting cytokines (Shafer-Weaver et al, 1996), a higher percentage of CD8 + lymphocytes that have a suppressive effect (Park et al, 1993;ShaferWeaver et al, 1996;Asai et al, 2000), changes in other cell types such as macrophages (Goddeeris et al, 1986;Nickerson, 1989), or memory cell phenotype (Bertotto et al, 1990;Taylor et al, 1994). Testing various antigens is needed to study the possibility of vaccinating dams to protect the newborn via adoptive cellular immunity.…”

Section: Discussionmentioning

confidence: 99%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum β-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a 3 H-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum β-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen-and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum β-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.

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Human breast milk T lymphocytes display the phenotype and functional characteristics of memory T cells

Cited by 96 publications

References 27 publications

TCF1 and LEF1 act as T-cell intrinsic HTLV-1 antagonists by targeting Tax

TCF1 and LEF1 act as T-cell intrinsic HTLV-1 antagonists by targeting Tax

Potential uses of milk epithelial cells: a review

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

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