Human Breast Milk-Derived Extracellular Vesicles in the Protection Against Experimental Necrotizing Enterocolitis

Pisano, Courtney; Galley, Jeffrey D.; Elbahrawy, Mostafa; Wang, Yijie; Farrell, Aidan; Brigstock, David R.; Besner, Gail E.

doi:10.1016/j.jpedsurg.2019.09.052

Cited by 79 publications

(75 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using the well-characterized rat NEC model, we have demonstrated that human BM-derived EVs reduce the incidence of NEC in rat pups when delivered orally with formula feeds. Orally delivered BM-EVs had improved efficacy compared to BM-EVs delivered intraperitoneally, which may be related to the stability of the BM-EVs trafficking through the digestive tract [51,86,90]. Human BM-EVs had pro-proliferative and anti-apoptotic effects in both rat (IEC-6) and human (FHS74) intestinal cell culture models, suggesting that the therapeutic properties of BM-EVs are mediated through a protective effect on intestinal epithelial cells [86].…”

Section: Breast Milk Extracellular Vesicles and Necrotizing Enterocolmentioning

confidence: 99%

“…In the search for exosomes/EVs that have a higher propensity for therapeutic effects, while also having the potential to be easily administered clinically, BM-derived exosomes have become a key player. Recently, there has been a spike in interest of the potential beneficial effects of BM exosomes [86,87]. These effects are spread across numerous mammals, including humans, bovines, camels, and pigeons [86][87][88][89].…”

Section: Breast Milk Exosomesmentioning

confidence: 99%

“…Recently, there has been a spike in interest of the potential beneficial effects of BM exosomes [86,87]. These effects are spread across numerous mammals, including humans, bovines, camels, and pigeons [86][87][88][89]. A particular focus is being paid to the effect that BM exosomes have on the gastrointestinal (GI) tract, given recent studies that have shown that these human BM exosomes are resilient to digestion and can be endocytosed by intestinal epithelial cells, indicating that BM exosomes have the ideal qualities of a potential therapeutic.…”

Section: Breast Milk Exosomesmentioning

confidence: 99%

“…Orally delivered BM-EVs had improved efficacy compared to BM-EVs delivered intraperitoneally, which may be related to the stability of the BM-EVs trafficking through the digestive tract [51,86,90]. Human BM-EVs had pro-proliferative and anti-apoptotic effects in both rat (IEC-6) and human (FHS74) intestinal cell culture models, suggesting that the therapeutic properties of BM-EVs are mediated through a protective effect on intestinal epithelial cells [86]. These results corroborated other research demonstrating that BM-EVs have similar protective effects on cellular proliferation and growth [87].…”

Section: Breast Milk Extracellular Vesicles and Necrotizing Enterocolmentioning

confidence: 99%

“…This knowledge will allow more stringent focusing of therapeutic EV cargos. As numerous studies have shown, NEC incidence and severity, as well as intestinal injury in commonly used in vitro models of gut disease, can be ameliorated through EV treatment [25,86,87]. The reductions in NEC injury severity by EVs may be mediated through EV-miRNAs, given their well-known ability to mediate disease outcomes [57].…”

Section: Future Directions Of Ev Therapy For Necmentioning

confidence: 99%

See 4 more Smart Citations

The Therapeutic Potential of Breast Milk-Derived Extracellular Vesicles

Galley

Besner

2020

Nutrients

Self Cite

View full text Add to dashboard Cite

In the past few decades, interest in the therapeutic benefits of exosomes and extracellular vesicles (EVs) has grown exponentially. Exosomes/EVs are small particles which are produced and exocytosed by cells throughout the body. They are loaded with active regulatory and stimulatory molecules from the parent cell including miRNAs and enzymes, making them prime targets in therapeutics and diagnostics. Breast milk, known for years to have beneficial health effects, contains a population of EVs which may mediate its therapeutic effects. This review offers an update on the therapeutic potential of exosomes/EVs in disease, with a focus on EVs present in human breast milk and their remedial effect in the gastrointestinal disease necrotizing enterocolitis. Additionally, the relationship between EV miRNAs, health, and disease will be examined, along with the potential for EVs and their miRNAs to be engineered for targeted treatments.

show abstract

Section: Breast Milk Extracellular Vesicles and Necrotizing Enterocolmentioning

confidence: 99%

Section: Breast Milk Exosomesmentioning

confidence: 99%

Section: Breast Milk Exosomesmentioning

confidence: 99%

Section: Breast Milk Extracellular Vesicles and Necrotizing Enterocolmentioning

confidence: 99%

Section: Future Directions Of Ev Therapy For Necmentioning

confidence: 99%

See 3 more Smart Citations

The Therapeutic Potential of Breast Milk-Derived Extracellular Vesicles

Galley

Besner

2020

Nutrients

Self Cite

View full text Add to dashboard Cite

show abstract

Developmental 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin exposure of either parent enhances the risk of necrotizing enterocolitis in neonatal mice

Mokshagundam

Ding

Rumph

et al. 2020

Birth Defects Research

View full text Add to dashboard Cite

Background: Necrotizing enterocolitis (NEC) is a rare, but potentially fatal intestinal inflammatory condition most often arising in premature infants. Infants provided formula are also at greater risk of developing this disease. Although the majority of formula-fed, preterm infants do not develop NEC, up to 30% of infants with the disease do not survive. Thus, identifying additional, currently unrecognized factors, which may predispose a specific infant to NEC development would be a significant clinical advancement. In this regard, we have previously reported that offspring of female or male mice with a history of developmental exposure to the environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exhibit altered sensitivity to inflammatory challenges and are frequently born premature. Herein, we examined the possibility that, compared to unexposed mice (F1 NONE), developmental TCDD exposure of either parent (maternal, F1M TCDD , or paternal, F1P TCDD) would enhance the risk of NEC in offspring (F2 TCDD mice) in association with supplemental formula feeding. Methods: Beginning on postnatal day 7, all neonates were randomized to maternal milk only or maternal milk with up to 20 supplemental formula feedings. All pups remained with the Dams and were additionally allowed to nurse ad libitum. Results: Formula-fed F2 NONE pups rarely developed NEC while this disease was common in formula-fed F2M TCDD and F2P TCDD mice. Unexpectedly, 50% of F2M TCDD pups that were not provided supplemental formula also developed NEC. Conclusions: Our studies provide evidence that a history of parental TCDD exposure enhances the risk of NEC in offspring and suggest exposure to environmental immunotoxicants such as TCDD may also contribute to this inflammatory disease in humans.

show abstract

Branched‐chain fatty acids in the vernix caseosa and meconium of infants born at different gestational ages

Liang

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

The functional lipid components found in breast milk, vernix caseosa, and meconium are Branched‐chain Fatty Acids (BCFA). The goal of this study was to establish the existence of BCFA in vernix and meconium in infants born at different gestational ages. TLC plates and GC‐MS were examined for the lipids in vernix caseosa and meconium. The results indicated that there were nine BCFA in vernix caseosa, including iso‐12:0, anteiso‐13:0, iso‐14:0, iso‐15:0, anteiso‐15:0, iso‐16:0, anteiso‐17:0, iso‐18:0, and iso‐20:0. Five BCFA (iso‐12:0, anteiso‐13:0, iso‐14:0, iso‐15:0, and anteiso‐15:0) were not contained in the meconium, suggesting that some of the BCFA may be digested and consumed by infants. In the vernix caseosa, the content of BCFA in triacylglycerol (TAG) and free fatty acid (FFA) was 15.59% and 11.82%, respectively. The vernix caseosa's wax ester fraction contained the highest content of BCFA, reaching up to 16.81%. The carbon chain length of fatty acids (FA) ranged from 12 to 24 in the vernix caseosa and 14 to 22 in meconium samples. The gestational age was likely to affect BCFA concentrations, with the vernix caseosa and meconium BCFA content being significantly higher in full‐term infants than in preterm infants (p < .001). Further research is required into the relationship between BCFA and gut microbiotas.

show abstract

Human Breast Milk-Derived Extracellular Vesicles in the Protection Against Experimental Necrotizing Enterocolitis

Cited by 79 publications

References 15 publications

The Therapeutic Potential of Breast Milk-Derived Extracellular Vesicles

The Therapeutic Potential of Breast Milk-Derived Extracellular Vesicles

Developmental 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin exposure of either parent enhances the risk of necrotizing enterocolitis in neonatal mice

Branched‐chain fatty acids in the vernix caseosa and meconium of infants born at different gestational ages

Contact Info

Product

Resources

About