Human Breast Adenocarcinoma Cytotoxicity and Modulation of 7,12-Dimethylbenz[<i>a</i>]anthracene-Induced Mammary Carcinoma in Balb/c Mice by <i>Acacia catechu</i> (L.f.) Wild Heartwood

Monga, Jitender; Chauhan, Chetan Singh; Sharma, Manu

doi:10.1177/1534735412463818

Cited by 17 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conclusion from the three studies of Monga et al (, , ) is that an aqueous, catechin‐rich heartwood extract of A. catechu exhibits potent anticancer activity as demonstrated by the prevention of squamous cell, mammary, and liver cancers in a dose‐dependent manner.…”

Section: Animal Studiesmentioning

confidence: 99%

“…The authors also conducted a phytochemical screening of the extract as described in the Section on Chemistry. Monga et al (2011Monga et al ( , 2013 have conducted a series of studies on the chemoprotective effects of A. catechu hardwood extract in mice. In each of these studies, 7,12-dimethylbenz[a]anthracene (DMBA) or DMBA plus 12-O-tetradecanoylphorbol-13-acetate was used to induce tumors in the mice.…”

Section: Animal Studiesmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant, Anti‐inflammatory, and Chemoprotective Properties of Acacia catechu Heartwood Extracts

Stohs

Bagchi

2015

Phytotherapy Research

View full text Add to dashboard Cite

Aqueous extracts of Acacia catechu heartwood are rich source of catechin and epicatechin (gallic acid derivatives), with smaller amounts of flavonoids. Extracts have also been prepared with ethyl acetate, ethanol, and methanol, and the properties of these extracts have been studied and are reviewed. Potent antioxidant activity has been well established in both in vitro and in vivo studies. This antioxidant activity is believed to be responsible for the anti-inflammatory, tissue protectant, antineoplastic, and analgesic activities that have been demonstrated and clearly established in animal and cell culture systems. Furthermore, antihyperglycemic, antidiarrheal, antinociceptive, and antipyretic activities have been demonstrated in animal studies. No adverse effects have been observed in animal or human studies or in cell culture systems. In spite of the fact that Acacia products have been used for many years and the general safety of catechins and epicatechins is well documented, few human studies have ever been conducted on the efficacy or safety of A. catechu heartwood extracts. Several studies have shown that a two-ingredient combination product containing A. catechu extract exhibited no adverse effects when administered daily for up to 12 weeks while exhibiting significant anti-inflammatory activity in subjects with osteoarthritis of the knee. There is a need for additional human clinical studies with regard to efficacy and safety.

show abstract

Section: Animal Studiesmentioning

confidence: 99%

Section: Animal Studiesmentioning

confidence: 99%

Antioxidant, Anti‐inflammatory, and Chemoprotective Properties of Acacia catechu Heartwood Extracts

Stohs

Bagchi

2015

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…(Fabaceae) also known as ‘Khadira’ has been extensively used as traditional medicine in India/Asia over a long period of time and a rich source of number of polyphenolic compounds. Previously, we have reported chemopreventive effect of aqueous extract of Acacia catechu in skin and mammary cancer rodent models [5,6]. (+)-Cyanidan-3-ol (CD-3) [3',4',5,7-tetrahydroxyflavan-3-ol] (Figure 1) is the most abundant polyphenolic flavonoid in the Acacia catechu heartwood and studies of the biological effects of CD-3 in cell culture and in vivo models indicated that this compound can inhibit lipid peroxidation [7].…”

Section: Introductionmentioning

confidence: 99%

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

et al. 2013

Self Cite

View full text Add to dashboard Cite

The objective of this study was to evaluate the cytotoxicity of (+)-cyanidan-3-ol (CD-3) in human hepatocellular carcinoma cell line (HepG2) and chemopreventive potential against hepatocellular carcinoma (HCC) in Balb/c mice. The HepG2 cell line was treated with CD-3 at various concentrations and the proliferation of the HepG2 cells was measure by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB) and lactate dehydrogenase (LDH) assays. Cell apoptosis was detected by Hoechst 33258 (HO), Acridine orange/ethylene dibromide (AO/EB) staining, DNA fragmentation analysis and the apoptosis rate was detected by flow cytometry. The HCC tumor model was established in mice by injecting N-nitrosodiethylamine/carbon tetrachloride (NDEA/CCl4) and the effect of CD-3 on tumor growth in-vivo was studied. The levels of liver injury markers, tumor markers, and oxidative stress were measured. The expression levels of apoptosis-related genes in in-vitro and in vivo models were determined by RT-PCR and ELISA. The CD-3 induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes under fluorescent microscopy and DNA fragmentation analysis. Annexin V/PI assay demonstrated that apoptosis increased with increase in the concentration of CD-3. The expression levels of apoptosis-related genes that belong to bcl-2 and caspase family were increased and AP-1 and NF-κB activities were significantly suppressed by CD-3. Immunohistochemistry data revealed less localization of p53, p65 and c-jun in CD-3 treated tumors as compared to localization in NDEA/CCl4 treated tumors. Taken together, our data demonstrated that CD-3 could significantly inhibit the proliferation of HepG2 cells in-vitro and suppress HCC tumor growth in-vivo by apoptosis induction.

show abstract

“…One such polyphenol, which has attracted a lot of interest in the last few years is (+)-catechin (Fig. 18 At the same time, we reported the growth inhibition and apoptosis induction potential of (+)-catechin against hepatocellular 19 and breast carcinoma. The (+)-catechin is widely distributed in various plants like Camellia sinensis (tea) and Acacia catechu Willd.…”

Section: Introductionmentioning

confidence: 76%

Topical (+)-catechin emulsified gel prevents DMBA/TPA-induced squamous cell carcinoma of the skin by modulating antioxidants and inflammatory biomarkers in BALB/c mice

et al. 2014

Self Cite

View full text Add to dashboard Cite

An emulsified gel of (+)-catechin was developed and evaluated topically against 7,12-dimethylbenz(a)anthracene-induced and 12-O-tetradecanoylphorbol-13-acetate-promoted (DMBA-induced and TPA-promoted) squamous cell carcinoma of the skin in BALB/c mice. The biological evaluation outcome indicated that the (+)-catechin emulsified gel increased the activity of oxidative stress biomarkers glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx), whereas it decreased the level of malondialdehyde (MDA). The mechanistic study showed that genes implicated in the inflammation and cancer, such as cyclooxygenase-2 (COX-2), nuclear factor-kappa B (NF-κB), and inducible nitric-oxide synthase (iNOS), were down-regulated by (+)-catechin emulsified gel while inhibiting an inflammatory mediator prostaglandin E2 (PGE2). The (+)-catechin emulsified gel further suppressed the activity of pro-inflammatory cytokines, viz. tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6). The in vitro permeation study revealed that release of (+)-catechin from an emulsified gel base reached a steady state after 6 h, while pH of the entire emulsified gel was found to be between 6.2 and 6.5 that falls well within the normal pH range of the skin.

show abstract

Human Breast Adenocarcinoma Cytotoxicity and Modulation of 7,12-Dimethylbenz[a]anthracene-Induced Mammary Carcinoma in Balb/c Mice by Acacia catechu (L.f.) Wild Heartwood

Cited by 17 publications

References 48 publications

Antioxidant, Anti‐inflammatory, and Chemoprotective Properties of Acacia catechu Heartwood Extracts

Antioxidant, Anti‐inflammatory, and Chemoprotective Properties of Acacia catechu Heartwood Extracts

Growth Inhibition and Apoptosis Induction by (+)-Cyanidan-3-ol in Hepatocellular Carcinoma

Topical (+)-catechin emulsified gel prevents DMBA/TPA-induced squamous cell carcinoma of the skin by modulating antioxidants and inflammatory biomarkers in BALB/c mice

Contact Info

Product

Resources

About