Human brain endothelial barrier cells are distinctly less vulnerable to silver nanoparticles toxicity than human blood vessel cells

Sokołowska, Paulina; Białkowska, Kamila; Siatkowska, Małgorzata; Rosowski, Marcin; Kucińska, Magdalena; Komorowski, Piotr; Makowski, Krzysztof; Walkowiak, Bogdan

doi:10.1016/j.nano.2017.05.015

Cited by 19 publications

(21 citation statements)

References 20 publications

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“…Additionally, for the reference cells, only a few microparticles are observed, that may be connected with microparticles transfer functioning of endothelium . Exposition of cells to SNPs causes association of these particles with cellular plasma membrane (marked with arrows in the Figure 7BCD), which was thoroughly discussed in our recent work . AFM images showed numerous irregular‐shaped cellular protrusions filled with SNPs agglomerates, formed as a step in the process of SNPs uptake.…”

Section: Discussionmentioning

confidence: 58%

“…Additionally, accumulation of SNPs inside endothelial cells can result in stiffening of cells detectable by AFS measurements with probes of different geometries. The uptake of SNPs by endothelial was revealed by increase of side (SSC) scattered light parameter obtained by flow cytometry in our previous paper . The presence of SNPs inside of cells was confirmed by TEM.…”

Section: Discussionmentioning

confidence: 60%

“…The imaging of SNPs was carried out by means of transmission electron microscopy, as shown in Figure 2A. The diameter of the nanoparticles is in the range of 30 to 100 nm, and a detailed analysis of the size of these nanoparticles was already published . DLS study of nanoparticles suspended in milliQ water showed 2 populations of particles, one with an average hydrodynamic radius of approximately 4 nm and a second with an average radius of 52 nm.…”

Section: Resultsmentioning

confidence: 99%

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Sensing of silver nanoparticles on/in endothelial cells using atomic force spectroscopy

Kołodziejczyk¹,

Jakubowska²,

Kucińska³

et al. 2018

J of Molecular Recognition

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Endothelial cells, due to their location, are interesting objects for atomic force spectroscopy study. They constitute a barrier between blood and vessel tissues located deeper, and therefore they are the first line of contact with various substances present in blood, eg, drugs or nanoparticles. This work intends to verify whether the mechanical response of immortalized human umbilical vein endothelial cells (EA.hy926), when exposed to silver nanoparticles, as measured using force spectroscopy, could be effectively used as a bio-indicator of the physiological state of the cells. Silver nanoparticles were characterized with transmission electron microscopy and dynamic light scattering techniques. Tetrazolium salt reduction test was used to determine cell viability after treatment with silver nanoparticles. An elasticity of native cells was examined in the Hanks' buffer whereas fixed cells were softly fixed with formaldehyde. Additional aspect of the work is the comparative force spectroscopy utilizing AFM probes of ball-shape and conical geometries, in order to understand what changes in cell elasticity, caused by SNPs, were detectable with each probe. As a supplement to elasticity studies, cell morphology observation by atomic force microscopy and detection of silver nanoparticles inside cells using transmission electron microscopy were also performed. Cells exposed to silver nanoparticles at the highest selected concentrations (3.6 μg/mL, 16 μg/mL) are less elastic. It may be associated with the reorganization of the cellular cytoskeleton and the "strengthening" of the cell cortex caused by presence of silver nanoparticles. This observation does not depend on cell fixation. Agglomerates of silver nanoparticles were observed on the cell membrane as well as inside the cells.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Sensing of silver nanoparticles on/in endothelial cells using atomic force spectroscopy

Kołodziejczyk¹,

Jakubowska²,

Kucińska³

et al. 2018

J of Molecular Recognition

Self Cite

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“…In vivo animal studies have revealed toxic effects of AgNPs in rodents by accumulating in their liver, spleen, and lung [56,57]. Similarly, AgNPs-mediated cytotoxicity in mammalian cells [55,58,59,60,61,62] depends greatly on the nanoparticle size, shape, surface charge, dosage, oxidation state, and agglomeration condition as well as the cell type. This article provides a state-of-the-art review on the recent development in the synthesis of AgNPs, their antibacterial activity, and cytotoxic effects in mammalian cells, especially in the past five years.…”

Section: Introductionmentioning

confidence: 99%

Bactericidal and Cytotoxic Properties of Silver Nanoparticles

Liao

Tjong

2019

IJMS

657

436

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Silver nanoparticles (AgNPs) can be synthesized from a variety of techniques including physical, chemical and biological routes. They have been widely used as nanomaterials for manufacturing cosmetic and healthcare products, antimicrobial textiles, wound dressings, antitumor drug carriers, etc. due to their excellent antimicrobial properties. Accordingly, AgNPs have gained access into our daily life, and the inevitable human exposure to these nanoparticles has raised concerns about their potential hazards to the environment, health, and safety in recent years. From in vitro cell cultivation tests, AgNPs have been reported to be toxic to several human cell lines including human bronchial epithelial cells, human umbilical vein endothelial cells, red blood cells, human peripheral blood mononuclear cells, immortal human keratinocytes, liver cells, etc. AgNPs induce a dose-, size- and time-dependent cytotoxicity, particularly for those with sizes ≤10 nm. Furthermore, AgNPs can cross the brain blood barrier of mice through the circulation system on the basis of in vivo animal tests. AgNPs tend to accumulate in mice organs such as liver, spleen, kidney and brain following intravenous, intraperitoneal, and intratracheal routes of administration. In this respect, AgNPs are considered a double-edged sword that can eliminate microorganisms but induce cytotoxicity in mammalian cells. This article provides a state-of-the-art review on the synthesis of AgNPs, and their applications in antimicrobial textile fabrics, food packaging films, and wound dressings. Particular attention is paid to the bactericidal activity and cytotoxic effect in mammalian cells.

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“…Figure 1D shows hydrodynamic radius distribution of SNPs prepared in cell culture medium. The diameter of the nanoparticles was in the range of 30 to 100 nm, and a detailed analysis of the size of these nanoparticles was already published 22 . From the DLS measurements (Figure 1B), three populations were recognized, of which the first probably referred to the nanoparticle stabilizer polyvinyl pyrrolidone (R approx.…”

Section: Resultsmentioning

confidence: 95%

Endothelial cell aging detection by means of atomic force spectroscopy

Kołodziejczyk¹,

Kucińska²,

Jakubowska³

et al. 2020

J of Molecular Recognition

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Endothelial cell aging is related to changes not only in cell phenotype, such as luminal changes, intimal and medial thickening, and increased vascular stiffness, but encompasses different cell responses to various substances including drugs or nanomaterials. In the present work, time‐ and dose‐dependent elasticity changes evoked by silver nanoparticles in endothelial cells in early (below 15) passages were analyzed. Silver nanoparticle concentrations of 3, 3.6, and 16 μg/mL were selected for elasticity measurements for long incubation (24 hours) and of 1 and 3 μg/mL for monitoring dynamic elasticity changes of 1‐, 3‐, and 6‐hour incubations. Surprisingly, a significant reduction in the cells elasticity modulus at lower number of passages exposed to silver nanoparticles used at 3 μg/mL for 24 hours was demonstrated. These results are in contrast to those obtained for endothelial cells in late (33‐43) passages that may result from cellular aging in response to nanosilver. Furthermore, for short incubation times (1 and 3 hours), SNP‐induced significant increase in the cell elasticity modulus was detected. In current work, we also attempted to answer the question whether the changes in cell elasticity were induced by the silver nanoparticles stabilized with polyvinyl pyrrolidone or by stabilizer itself. Elasticity measurements were supplemented by observations made with transmission electron microscopy and scanning electron microscopy, which confirmed the presence of silver nanoparticles inside the cells and on the cell membrane. Additionally, activation of reactive oxygen species was detected for cells exposed to SNPs for 1 and 3 hours, which was accompanied by increased cell elasticity modulus suggesting a possible mechanism of observed phenomenon.

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Human brain endothelial barrier cells are distinctly less vulnerable to silver nanoparticles toxicity than human blood vessel cells

Cited by 19 publications

References 20 publications

Sensing of silver nanoparticles on/in endothelial cells using atomic force spectroscopy

Sensing of silver nanoparticles on/in endothelial cells using atomic force spectroscopy

Bactericidal and Cytotoxic Properties of Silver Nanoparticles

Endothelial cell aging detection by means of atomic force spectroscopy

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