Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering

Ciapetti, G.; Ambrosio, Luigi; Marletta, Giovanni; Baldini, Nicola; Giunti, Armando

doi:10.1016/j.biomaterials.2006.08.025

Cited by 95 publications

(68 citation statements)

References 38 publications

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“…Human stromal cells were isolated from surgical human bone specimens obtained by routine clinical practice with the informed consent of the patients as described previously (Ciapetti et al 2006). For the evaluation of osteoblasts content, bone stromal cells were fixed in 4% formaldehyde in 0.1 phosphate buffer, pH 7.2, and then incubated for 30 min with a solution of 2% Fast violet B salt (Sigma) and 4% Naphthol AS-MX phosphate alkaline solution (Sigma).…”

Section: Cell Culturementioning

confidence: 99%

Arachidonic acid modulates the crosstalk between prostate carcinoma and bone stromal cells

Angelucci

Garofalo²,

Speca³

et al. 2008

Endocrine Related Cancer

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Diets high in n-6 fatty acids are associated with an increased risk of bone metastasis from prostate carces (PCa). The molecular mechanism underlying this phenomenon is largely unknown. Arachidonic acid (AA) and its precursor linoleic acid can be metabolized to produce pro-inflammatory cytokines that act as autocrine and paracrine regulators of cancer behavior. We and other authors have previously reported that factors released by PCa cells excite an aberrant response in bone marrow stromal cells (BMSCs). We planned to study how AA may modulate in vitro the interaction between PCa cells and human BMSCs. First, we observed that AA is a potent mitogenic factor for PCa cells through the production of both 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) metabolites. While 5-LOX controls cell survival through the regulation of the Bcl-2/Bax ratio, COX-2 activity stimulates the release of transforming growth factor-a (TGF-a) and pro-inflammatory cytokines. The blockade of COX-2 activity through a specific inhibitor is sufficient to repress AA-induced gene transcription. The over-expression of transforming growth factor -a (TGF-a), tumour necrosis factor-a (TNF-a) and interleukin-1 b (IL-1b) by AA-primed PCa cells resulted particularly effective in modifying cell behavior of cultured human BMSCs. In fact, we observed an increment in the cell number of BMSCs, due prevalently to the action of TGF-a, the number of osteoblasts, and the production of receptor activator for nuclear factor k B ligand (RANKL), events mainly controlled by inflammatory cytokines. These findings provide a possible molecular mechanism by which dietary n-6 fatty acids accumulating in bone marrow may influence the formation of PCaderived metastatic lesions and indicate new molecular targets for the therapy of metastatic PCa.

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Section: Cell Culturementioning

confidence: 99%

Arachidonic acid modulates the crosstalk between prostate carcinoma and bone stromal cells

Angelucci

Garofalo²,

Speca³

et al. 2008

Endocrine Related Cancer

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“…After 14 days of culture in osteogenic medium, B10 MSCs differentiated into osteoblasts and this was confirmed by strong alkaline phosphatase staining (Figure 2c). 14,15 Differentiation of B10 MSCs into chondroblasts was determined by staining the cell pellets with Alcian blue, which positively identifies proteoglycan extracellular matrix, specific components of cartilage tissues ( Figure 2c). As in primary human MSCs, B10 MSCs expressed markers of adipocytes, of osteoblasts and of chondrocytes following culture under the appropriate differentiation-inducing conditions.…”

Section: Differentiation Of Human Mscsmentioning

confidence: 99%

Potential differentiation of human mesenchymal stem cell transplanted in rat corpus cavernosum toward endothelial or smooth muscle cells

Song¹,

Lee

Park

et al. 2007

Int J Impot Res

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One of the causes of erectile dysfunction (ED) is the damaged penile cavernous smooth muscle cells (SMCs) and sinus endothelial cells (ECs). To investigate the feasibility of applying immortalized human mesenchymal stem cells (MSCs) to penile cavernous ECs or SMCs repair in the treatment of ED, the in vivo potential differentiation of the immortalized human MSCs toward penile cavernous endothelial or smooth muscle was investigated. One clone of immortalized human bone marrow mesenchymal stem cell line B10 cells via retroviral vector encoding v-myc were transplanted into the cavernosum of the Sprague-Dawley rats and harvested 2 weeks later. The expression of CD31, von Willebrand factor (vWF), smooth muscle cell actin (SMA), calponin and desmin was determined immunohistochemically in rat penile cavernosum. Multipotency of B10 to adipogenic, osteogenic or chondrogenic differentiation was found. Expression of EC specific markers (CD31 or vWF protein) and expression of SMC specific markers (calponin, SMA or desmin protein) were demonstrated in grafted B10 cells. When human MSCs were transplanted into the penile cavernosum, they have the potential to differentiate toward ECs or SMCs. Human MSCs may be a good candidate in the treatment of penile cavernosum injury.

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“…Bone-marrow-derived mesenchymal stem cells (BMMSCs) have received attention because of their potential utility in bone regeneration (Ciapetti et al, 2006;Brooke et al, 2007). The cells have self-renewal as well as pluripotent capacities, which can differentiate into distinct cell types, including osteocytes, adipocytes, and chondrocytes (Prockop, 1997;Ferrari et al, 1998;Dennis et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Bone Regeneration at Dental Implant Sites with Suspended Stem Cells

Zheng

Park

Cho³

et al. 2014

J Dent Res

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During the maintenance of bone marrow-derived mesenchymal stem cells (BMMSCs), suspended cells are discarded normally. We noted the osteogenic potential of these cells to be like that of anchorage-dependent BMMSCs. Therefore, we characterized suspended BMMSCs from rabbit bone marrow by bioengineering and applied the suspended BMMSCs to double-canaled dental implants inserted into rabbits. After primary isolation of BMMSCs, we collected the suspended cells during primary culture on the third day. The cells were transferred and maintained on an extracellular-matrix-coated culture plate. The cells were characterized and compared with BMMSCs by colony-forming-unit fibroblast (CFU-f) and cell proliferation assay, fluorescence-activated cell sorter (FACS), in vitro multipotency, and reverse transcription polymerase chain reaction (RT-PCR). We also analyzed the osteogenic potential of cells mixed with hydroxyapatite/tricalcium phosphate (HA/TCP) and transplanted into immunocompromised mice. We compared the viability and proliferation of the suspended BMMSCs and BMMSCs on the titanium implant surface and observed cell morphology. Then, the cells mixed with HA/TCP were applied to the double-canaled implants during installation into rabbit tibia. Four weeks later, we analyzed bone formation inside the canal by histomorphometry. The suspended cells showed higher CFU-f on the extracellular matrix (ECM)-coated culture plate and similar results of proliferation capacity compared with BMMSCs. The cells also showed osteogenic, adipogenic, and chondrogenic ability. The suspended cells showed levels of attachment survival and proliferation on the surfaces of titanium implant discs to be higher than or similar to those of BMMSCs. The suspended cells as well as BMMSCs showed stronger bone formation ability in both upper and lower canals of the implants compared with controls on double-canaled implants inserted into rabbit tibia. In this study, we showed that suspended cells after primary BMMSC isolation have bone regeneration capacity like that of BMMSCs, not only in vitro but also in vivo. ECM was valuable for propagation of MSCs for cell-based bone regeneration. Therefore, the suspended cells could also be useful tools for bone regeneration after implant surgery.

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Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering

Cited by 95 publications

References 38 publications

Arachidonic acid modulates the crosstalk between prostate carcinoma and bone stromal cells

Arachidonic acid modulates the crosstalk between prostate carcinoma and bone stromal cells

Potential differentiation of human mesenchymal stem cell transplanted in rat corpus cavernosum toward endothelial or smooth muscle cells

Bone Regeneration at Dental Implant Sites with Suspended Stem Cells

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