Human Blood Monocytes Interact with Type I Collagen Through αxβ2 Integrin (CD11c-CD18, gp150-95)

Garnotel, Roselyne; Rittié, Laure; Poitevin, Stéphane; Monboisse, Jean-Claude; Nguyên, Philippe; Potron, G; Maquart, F X; Randoux, A; Gillery, Philippe

doi:10.4049/jimmunol.164.11.5928

Cited by 78 publications

(65 citation statements)

References 33 publications

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“…Finally, the leukocytes cross the endothelial cell layer and underlying BM and adhere and migrate within the extravascular tissue. 1 However, Mac-1, ␣ X ␤ 2 , and ␣ D ␤ 2 also bind to other proteins: Mac-1 to inactivated complement factor (iC3b), fibrinogen, factor X, ␣ X ␤ 2 to iC3b and type I collagen, 38 and ␣ D ␤ 2 to VCAM-1. 39 In addition, Mac-1 and ␣ X ␤ 2 can interact specifically with various polysaccharides, such as ␤-glucan, mannose, N-acetyl-D-glucosamine, and glucose, which mediate attachment to bacteria.…”

Section: Ablation Of Integrin Genes In Mice and Their Resulting Phenomentioning

confidence: 99%

Functional Consequences of Integrin Gene Mutations in Mice

Bouvard

Brakebusch

Gustafsson

et al. 2001

Circulation Research

212

147

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Section: Ablation Of Integrin Genes In Mice and Their Resulting Phenomentioning

confidence: 99%

Functional Consequences of Integrin Gene Mutations in Mice

Bouvard

Brakebusch

Gustafsson

et al. 2001

Circulation Research

212

147

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“…[18][19][20][21][22][23][24][25] The functional role and ligands of CD11c/CD18 have not been well defined but appear to be similar to those of Mac-1, ie, implicated in adhesion of monocytes to endothelium 26 and binding to iC3b, fibrinogen, LPS, and type I collagen. [27][28][29][30] Human CD23, the low-affinity receptor for IgE (Fc⑀RII), is a 45-kd type II membrane glycoprotein expressed on many hematopoietic cell types, including B lymphocytes and monocytes. CD23 is cleaved into biologically active soluble fragments (sCD23), and this results in generation of a relatively stable 25-kd fragment.…”

Section: Introductionmentioning

confidence: 99%

Ligation of CD11b and CD11c β2 integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1α (MIP-1α) and MIP-1β production in primary human monocytes through a pathway dependent on nuclear factor–κB

Rezzonico

Imbert

Chicheportiche

et al. 2001

Blood

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Chemokines and adhesion molecules such as integrins play a major part in the trafficking, extravasation, and recruitment of leukocytes to inflammatory sites. This study investigated the effects of ␤ 2 integrin engagement on chemokine production by freshly isolated human monocytes. We found that ligation of CD11b or IntroductionRecruitment of circulating leukocytes to an inflammatory site in response to stimuli such as infectious agents (viruses, bacteria, and protozoans) or noninfectious processes (trauma, autoimmune disorders, and ischemia-reperfusion injury) is a crucial step in the development of both acute and chronic inflammatory responses. In all these conditions, extravasation of circulating leukocytes requires communication with vascular endothelial cells that in turn depends on an interrelated network of events involving the finely regulated action of inflammatory cytokines (ie, interleukin [IL] 1 and tumor necrosis factor [TNF]-␣), adhesion molecules (notably integrins), and chemoattractant cytokines called chemokines.Chemokines are highly conserved, small, secreted or membranebound cytokines with molecular masses of 6 to 14 kd and a characteristic 4-cysteine motif in their amino acid sequence. [1][2][3] Two main subfamilies are defined according to the position of the first 2 cysteine residues. In the CXC (or ␣-chemokine) subfamily, these residues are separated by one amino acid, whereas in the CC (or ␤-chemokine) subfamily, they are adjacent. The ␤-chemokines, which include monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein (MIP) 1␣, MIP-1␤, and regulated on activation, normal T-cell expressed and secreted (RANTES) molecules, are potent chemotactic agents for monocytes. 4,5 The migratory behavior of monocytes in response to a concentration gradient of these soluble chemotactic factors also depends on adhesion molecules, particularly the leukocyte-specific ␤ 2 integrins.Integrins are part of a family of heterodimeric transmembrane glycoproteins that recognize a variety of ligands or counterreceptors, including extracellular matrix and cell-surface and plasma proteins, and that consequently control numerous physiologic functions, such as adhesion, locomotion, chemotaxis, and phagocytosis. 6 Expression of ␤ 2 integrins is restricted to leukocytes, with distribution varying among leukocyte subtypes. The ␤ 2 integrins share a common ␤ chain (CD18) and have at least 4 distinct ␣ chains noncovalently associated with CD18: lymphocyte functional antigen 1 (␣ L ␤ 2 , CD11a/CD18), macrophage antigen 1 (Mac-1; ␣ M ␤ 2 , CR3, CD11b/CD18), p150,95 (␣ X ␤ 2 , CR4, CD11c/ CD18), and a newly characterized member, ␣ D ␤ 2 (CD11d/ CD18). 7-9 Interestingly, some chemokines (MCP-1, MIP-1␣, MIP-1␤, and RANTES) increase surface expression of CD11b/CD18 and CD11c/CD18 on human monocytes. [10][11][12] The best characterized ligands of CD11a/CD18 are intracellular adhesion molecule (ICAM) 1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50), all of which are members of the immunoglobulin superfamily. [13][14...

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“…Acid-extracted, nonpepsinized collagen I from rat tail tendons was prepared as described in [12]. Lyophilized collagen I was dissolved in sterile 0.1% acetic acid (3 mg/mL).…”

Section: Fluorescence Videomicroscopy On Collagen Gelsmentioning

confidence: 99%

FTIR protein secondary structure analysis of human ascending aortic tissues

Bonnier

Rubin

Debelle

et al. 2008

Journal of Biophotonics

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Journal of BIOPHOTONICSThe advent of moderate dilatations in ascending aortas is often accompanied by structural modifications of the main components of the aortic tissue, elastin and collagen. In this study, we have undertaken an approach based on FTIR microscopy coupled to a curve-fitting procedure to analyze secondary structure modifications in these proteins in human normal and pathological aortic tissues. We found that the outcome of the aortic pathology is strongly influenced by these proteins, which are abundant in the media of the aortic wall, and that the advent of an aortic dilatation is generally accompanied by a decrease of parallel b-sheet structures. Elastin, essentially composed of b-sheet structures, seems to be directly related to these changes and therefore indicative of the elastic alteration of the aortic wall. Conventional microscopy and confocal fluorescence microscopy were used to compare FTIR microscopy results with the organization of the elastic fibers present in the tissues. This in-vitro study on 6 patients (three normal and three pathologic), suggests that such a spectroscopic marker, specific to aneurismal tissue characterization, could be important information for surgeons who face the dilemma of moderate aortic tissue dilatation of the ascending aortas.Infrared spectroscopy and imaging was applied to analyse Human thoracic ascending aortas with the aim to highlight the protein secondary structure reorganisation after an aneurism outcome. We used a curve-fitting procedure to visualise modifications in the protein spectral profile where alteration of b structures in pathological aortas could be detected. Our spectroscopic observations on tissue sections corroborated with confocal fluorescence microscopy clearly demonstrating that elastic fibres of aortic wall are strongly altered. Elastin appears as the privileged target during the pathological process.

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Human Blood Monocytes Interact with Type I Collagen Through αxβ2 Integrin (CD11c-CD18, gp150-95)

Abstract: This information is current as (CD11c-CD18, gp150-95) Integrin 2 β x α I Collagen Through

Cited by 78 publications

References 33 publications

Functional Consequences of Integrin Gene Mutations in Mice

Functional Consequences of Integrin Gene Mutations in Mice

Ligation of CD11b and CD11c β2 integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1α (MIP-1α) and MIP-1β production in primary human monocytes through a pathway dependent on nuclear factor–κB

FTIR protein secondary structure analysis of human ascending aortic tissues

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