Human blood-derived fibrin releasates: Composition and use for the culture of cell lines and human primary cells

Burnouf, Thierry; Lee, Chang Yu; Luo, Chin Wan; Kuo, Ya Po; Chou, Ming Li; Wu, Yu Wen; Tseng, Yu Ting; Su, Chun-Han

doi:10.1016/j.biologicals.2011.09.017

Cited by 36 publications

(33 citation statements)

References 40 publications

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“…The capacity of two human blood fractions (the supernatant serum and the releasate PRF) were evaluated monitoring the cell proliferation in two human cell lines, human embryonic kidney fibroblasts HEK293 and human MG-63 osteoblastic cell line. The supernatant serum and the releasate PRF at 1-10%, stimulated cell growth more significantly than FBSfree medium [78]. It is interesting that the addition of platelet lysateplasma, obtained from pooled PRP, to human mesenchymal stromal cell (hMSCs) culture showed a consistently greater proliferation rate than with FBS at the same percentage [79].…”

Section: Discussionmentioning

confidence: 99%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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SummaryScientific background: Platelet concentrates are nowadays widely applied in different clinical fields to improve soft tissue and bone regeneration. "Concentrated Growth Factors" (CGF) is a new generation of platelet concentrate products, which exhibits an interesting clinical and biotechnological application potential. Aim of the study:The aim of this study is to assess the biological rationale for the use of CGF, by evaluating blood cell localization, the in vitro cumulative release of seven growth factors (PDGF-AB, VEGF, TNF-α, TGF-β1, BDNF, BMP-2 and IGF-1), its in vitro effects on cell proliferation and its mechanical behavior.Methods: CGFs were obtained from volunteer donors. Blood cell localization was evaluated after properly morphological staining and immunohistochemistry. The amount of growth factors release was measured at 5 hours, 1, 3, 6, 7 and 8 days, using ELISA assay. Cells were cultured with and without CGF and their proliferation was evaluated after 72 hours, performing the quantification of Ki-67, using flow cytometry (FACS). The mechanical response of CGF under compression was also attempted. Results:The results showed that platelets and leukocytes were found in a very thin space called "buffy coat", localized between the white and red part of CGF. Each growth factor evaluated, had a specific kinetic release with a great variability among subjects. The in vitro cell proliferation was stimulated. CGF showed an "apparent plasticity" and its mechanical response was influenced by fibrin network structure. Conclusion:These findings support the CGF's clinical use and will allow us to better understand and improve the clinical outcomes. B io lo g y and M e d ic in e

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Section: Discussionmentioning

confidence: 99%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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“…In addition, trophic factors for the differentiation of stem cells are increased in such irritated coelomic fluids [2,13,16,17,18] and seem to contribute to specific differentiation of stem cells derived from the blood stream, as shown by our analysis.…”

Section: Discussionmentioning

confidence: 78%

“…We have already demonstrated that body cavity fluid can induce EGFR and keratin expression in peripheral blood stem cells in vitro [2]. As described previously, all of the body cavity fluids with CD34+ cells had fibrinous exudate rich in EGF and other trophic factors [13] and seemed to have enhanced ability to induce EGFR and/or keratin CD34+ stem cells of peripheral blood origin.…”

Section: Discussionmentioning

confidence: 97%

Cytologic and Immunophenotypic Features of CD34+ Stem Cells in Body Cavity Fluids

Kataoka

Okabe²,

Amano³

et al. 2012

Acta Cytologica

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Objectives: The possibility of leakage of CD34+ bone marrow stem cells from the peripheral blood into the coelomic cavity and the capability of coelomic fluid factors to induce their non-hematogenous differentiation were examined by immunocytochemistry (ICC). Study Design: Body cavity fluid smears from 12 and 18 patients with and without cancer, respectively, were processed for double immunoperoxidase or double fluorescent ICC methods using antibodies against CD34, CD14, CD16, CD68, AE1/AE3, epidermal growth factor receptor (EGFR), D2-40, and CA125. Results: Heavily irritated exudative fluid from 6 patients with or without cancer contained a few small round cells positive for CD34. Some of them co-expressed myeloid or monocytic markers such as CD14, CD68 or CD16. Some of the CD34+ cells also co-expressed AE1/AE3 or EGFR. In addition, D2-40 and CA125 were also demonstrated though the expression of the latter was quite sporadic. Conclusion: These findings support the concept that CD34+ stem cells can be released into irritated body cavity fluid and the possibility of subsequent differentiation to a non-hematogenous lineage under the influence of local humoral factors, in agreement with our previous in vitro experiments. The possibility of such a phenomenon should be kept in mind when body cavity fluid specimens are analyzed by ICC for diagnostic purposes.

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“…PRF contains high levels of GF's that play a central role in hemostasis and the bone healing process, including vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), insulin-like growth factor (IGF), epidermal growth factor (EGF) and transforming growth factor (TGF) [8]. These GF's were gradually delivered in a controlled manner and inducing osteogenesis through osteoconduction, osteoinduction and angiogenesis, respectively.…”

Section: -4mentioning

confidence: 99%

“…Another advantage possessed by hydrogel CHA lies in its ability to be drug delivery system in controlled release technology [7]. Platelet-rich fibrin (PRF) is one of the latest generation of platelet concentrate which contains high levels GF that plays a central role in tissue engineering applications to induce hemostasis and the bone healing process [8]. In vitro study proved PRF may release autologous GF's gradually and express stronger effect on proliferation and differentiation of osteoblasts than platelet rich plasma [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

Alhasyimi¹,

Pudyani²,

Asmara³

et al. 2018

AIP Conference Proceedings

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Human blood-derived fibrin releasates: Composition and use for the culture of cell lines and human primary cells

Cited by 36 publications

References 40 publications

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Cytologic and Immunophenotypic Features of CD34+ Stem Cells in Body Cavity Fluids

Effect of carbonated hydroxyapatite incorporated advanced platelet rich fibrin intrasulcular injection on the alkaline phosphatase level during orthodontic relapse

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