Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds

Sanapalli, Bharat Kumar Reddy; Yele, Vidyasrilekha; Singh, Mantosh Kumar; Thumbooru, Shilpa.N.; Parvathaneni, Madhukiran; Karri, Veera Venkata Satyanarayana Reddy

doi:10.1016/j.biopha.2023.114540

Cited by 8 publications

(2 citation statements)

References 75 publications

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“…Similarly, 10-week treatment of HFD-fed mice with the modified HD5 1–9 fragment, D3, improved glucose clearance rate comparable to those of normal chow-fed mice, indicating that the HD5 1–9 fragment, D3, may improve obesity-induced insulin resistance [ 37 ]. Similarly, hBD2 was found to regulate diabetic wound healing in rats, as hBD2-loaded Poly-lactic-co-glycolic acid (PLGA) nanoparticles accelerated healing, which was associated with decreased Mmp9 and TNFa expression [ 54 ]. However, our results provide evidence for the first time that hBD2, comparable to HD5 1–9 , also modulates glucoregulatory capacity in WSDF-fed mice and thus could be an interesting therapeutic approach for diet-induced disturbances in glucose metabolism.…”

Section: Discussionmentioning

confidence: 99%

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Filipe Rosa,

Rings,

Stolzer

et al. 2023

IJMS

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Obesity and metabolic comorbidities are associated with gut permeability. While high-fructose and Western-style diet (WSD) disrupt intestinal barrier function, oral administration of human α-defensin 5 (HD5) and β-defensin 2 (hBD2) is believed to improve intestinal integrity and metabolic disorders. Eighty-four male C57BL/6J mice were fed a WSD or a control diet (CD) ± fructose (F) for 18 weeks. In week 13, mice were randomly divided into three intervention groups, receiving defensin fragment HD51–9, full-length hBD2, or bovine serum albumin (BSA)-control for six weeks. Subsequently, parameters of hepatic steatosis, glucose metabolism, and gut barrier function were assessed. WSDF increased body weight and hepatic steatosis (p < 0.01) compared to CD-fed mice, whereas peptide intervention decreased liver fat (p < 0.05) and number of hepatic lipid droplets (p < 0.01) compared to BSA-control. In addition, both peptides attenuated glucose intolerance by reducing blood glucose curves in WSDF-fed mice. Evaluation of gut barrier function revealed that HD51–9 and hBD2 improve intestinal integrity by upregulating tight junction and mucin expression. Moreover, peptide treatment restored ileal host defense peptides (HDP) expression, likely by modulating the Wnt, Myd88, p38, and Jak/STAT pathways. These findings strongly suggest that α- and β-defensin treatment improve hepatic steatosis, glucose metabolism, and gut barrier function.

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Section: Discussionmentioning

confidence: 99%

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Filipe Rosa,

Rings,

Stolzer

et al. 2023

IJMS

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“…Its hydrophobic nature does not offer itself a suitable platform for cell adherence, but it can be functionalized or associated with other biopolymeric materials or particles/molecules to improve its biofunctionality. The glass transition temperature (Tg) of these polymers is around 37 • C. Also, PLGA has successfully been used for decades in several skin and tissue engineering applications as nanoparticles [122,123], microparticles [124], electrospun co-axial fibers [125], bilayer electrospun membranes [126], nanosuspensions [127], thermogel dressing [128], hydrogels [129], microspheres [130], composite biofilms [131] and nanocomposites scaffolds [132].…”

Section: Poly(lactide-co-glycolide) Plgamentioning

confidence: 99%

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

Elango,

Zamora-Ledezma,

Maté-Sánchez de Val

2023

J. Compos. Sci.

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Modern research has evolved several approaches toward skin regeneration and one of the novel concerns is the use of polymer-based systems due to their excellent beneficial properties to the skin. Several polymers, such as cellulose, hyaluronan, alginate, chitosan, collagen, fibrin and fibroin, have been tested and have proven the benefits for skin regeneration, and most of them are derived from either polysaccharide- or protein-based materials. In order to understand the mode of action, several researchers investigated the cell–matrix interaction and possible signaling mechanism in skin regeneration. Not only the signaling mechanism but also the mode of cell communication determines the application of polysaccharide- and protein-based polymers in practice. Based on the above significance, this review disclosed the recent findings to compile a possible method of communication between cells and polymers derived from polysaccharide-based (such as cellulose, hyaluronan, chitosan, alginate, agar, and xanthan gum) and protein-based (such as collagen, gelatin, fibrin, and silk fibroin) materials along with other polymers, such as poly(vinyl alcohol), polyglycolide or poly(glycolic acid), or poly(lactic acid) in skin regeneration. Accordingly, this review addresses the fundamental concept of cell–matrix communication, which helps us to understand the basis of the polymer’s functions in the biomedical field.

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Approaches to Control and Monitor Protease Levels in Chronic Wounds

Kolahreez,

Ghasemi‐Mobarakeh,

Liebner

et al. 2024

Advanced Therapeutics

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Some wounds do not follow the typical healing trajectory and show very little, if any, progress. They dreadfully threaten the patients' quality of life and may even lead to limb amputation and death. Several interrelated systemic and local factors may cause a delay in wound healing. The microenvironment of these wounds could be different from wounds that heal spontaneously or with standard treatment. As discussed in the present work, proteases’ levels or activity could be elevated in some cases of chronic ulcers; infection could aggravate the situation. A detailed overview of the proteases’ effect on wound healing is presented in this paper. Assuming untimely or irregularly excessive proteolytic activity plays a pathological role, some researchers targeted attenuation of protease level as a strategy to promote healing. This could be done via different approaches, including physical removal, inhibiting their activity, and controlling their expression directly through gene silencing or indirectly, for instance, by resolving infection. Apart from targeting protease modulation as a therapeutic strategy, many studies have examined the protease state to gain insights into the underlying mechanisms by which a remedy, dressing, or specific therapy has affected the healing process. This article aims to provide an overview of the studies in these two categories. Of course, various proteases are involved in the healing process, but in each study, usually, only one or some of them were investigated; matrix metalloproteinase‐2 (MMP‐2), MMP‐9, and human neutrophil elastase (HNE) are among them. Furthermore, in some cases, cumulative proteolytic activity has been evaluated in terms of gelatinolytic, collagenolytic, or caseinolytic activity, which is also absolutely beneficial. Some research groups have considered the potential of proteases as a diagnostic or prognostic biomarker and have tried to develop some sensors or indicators; this topic has also been covered in the present work.This article is protected by copyright. All rights reserved

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Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds

Cited by 8 publications

References 75 publications

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Human α-Defensin 51–9 and Human β-Defensin 2 Improve Metabolic Parameters and Gut Barrier Function in Mice Fed a Western-Style Diet

Natural vs Synthetic Polymers: How Do They Communicate with Cells for Skin Regeneration—A Review

Approaches to Control and Monitor Protease Levels in Chronic Wounds

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