Human axial progenitors generate trunk neural crest cells

Frith, Thomas J.R.; Granata, Ilaria; Stout, Erin; Wind, Matthew; Thompson, Oliver; Neumann, Katrin; Stavish, Dylan; Pr, Heath; Jo, Hackland; Anastassiadis, Konstantinos; Gouti, Mina; Briscoe, James; Wilson, Marcus D.; Mr, Guarracino; Pw, Andrews; Tsakiridis, Anestis

doi:10.1101/272591

Cited by 2 publications

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“…Recent work on multipotent zebrafish neural crest cells suggests that at least a portion of the neural biased trunk neural crest (NC) progenitors arise from early neural biased zebrafish NMps at 5-6ss ( Lukoseviciute et al., 2021 ). A similar conclusion was reached in multiple studies of in vitro human pluripotent stem cell-derived axial progenitors, demonstrating that the generation of trunk NCs involves an obligatory NMp intermediate ( Frith et al., 2018 ; Hackland et al., 2019 ). We identified sp5l , cdh6 , znf703 and foxd3 as differentially expressed genes of the NMp cluster; all of which have important roles in neural crest specification.…”

Section: Discussionsupporting

confidence: 71%

Zebrafish neuromesodermal progenitors undergo a critical state transition in vivo

et al. 2022

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Section: Discussionsupporting

confidence: 71%

Zebrafish neuromesodermal progenitors undergo a critical state transition in vivo

et al. 2022

View full text Add to dashboard Cite

“…Recent work on multipotent zebrafish neural crest cells suggests that at least a portion of the neural biased trunk neural crest (NC) progenitors arise from early neural biased zebrafish NMps at 5-6ss (Lukoseviciute et al, 2021). A similar conclusion was reached in multiple studies of in vitro human pluripotent stem cellderived axial progenitors, demonstrating that the generation of trunk NCs involves an obligatory NMp intermediate (Frith et al, 2018;Hackland et al, 2019). We identified sp5l, cdh6, znf703 and foxd3 as differentially expressed genes of the NMp cluster; all of which have important roles in neural crest specification.…”

Section: Discussionmentioning

confidence: 72%

Zebrafish Neuromesodermal Progenitors Undergo a Critical State Transition in vivo

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Saunders

Verd

et al. 2022

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The transition state model of cell differentiation proposes that a transient window of gene expression stochasticity precedes entry into a differentiated state. As this has been assessed primarily in vitro, we sought to explore whether it can also be observed in vivo. Zebrafish neuromesodermal progenitors (NMps) differentiate into spinal cord and paraxial mesoderm at the late somitogenesis stages. We observed an increase in gene expression variability at the 24 somite stage (24ss) prior to their differentiation. From our analysis of a published 18ss scRNA-seq dataset, we showed that the NMp population possesses a signature consistent with a population undergoing a critical transition. By building in silico composite gene expression maps from our image data, we were able to assign an NM index to each in silico NMp based on the cumulative expression of its neural and mesodermal markers. With the NM index distributions, we demonstrated that cell population heterogeneity of the NMps peaked at 24ss. We then incorporated stochasticity and non-autonomy into a genetic toggle switch model and uncovered the existence of rebellious cells, which we then confirmed by reexamining the composite maps. Taken together, our work supports the transition state model within an endogenous cell fate decision making event.

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Human axial progenitors generate trunk neural crest cells

Abstract: 29The neural crest (NC) is a multipotent embryonic cell population generating distinct cell types 30 in an axial position-dependent manner. The production of NC cells from human pluripotent 31 stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin 32

Cited by 2 publications

References 96 publications

Zebrafish neuromesodermal progenitors undergo a critical state transition in vivo

Zebrafish neuromesodermal progenitors undergo a critical state transition in vivo

Zebrafish Neuromesodermal Progenitors Undergo a Critical State Transition in vivo

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