Human as the Ultimate Wound Healing Model: Strategies for Studies Investigating the Dermal Lipidome

Wijesinghe, Dayanjan S.; Warncke, Urszula Osinska; Diegelmann, Robert F.

doi:10.1007/s13671-016-0156-3

Cited by 8 publications

(5 citation statements)

References 77 publications

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“…Recently, it was demonstrated that long chain fatty acids and cholesterol were correlated with faster recovery from transepidermal water loss and SC hydration . While bulk methods such as Raman spectroscopy and liquid chromatography have shown promises in the study of the wound healing research, , they lack the specificity to identify and map molecular components during the skin healing process. On the other hand, mass spectrometry imaging (MSI) has been shown to be very informative, generating molecular maps with high spatial resolution (e.g., using TOF-SIMS , ) and the distribution of a wide variety of molecular components (e.g., using matrix-assisted laser desorption-ionization, MALDI , ).…”

mentioning

confidence: 99%

Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing

et al. 2019

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Skin repair is a significant aspect of human health. While the makeup of healthy stratum corneum and epidermis is generally understood, the mobilization of molecular components during skin repair remains largely unknown. In the present work, we utilize multimodal, in situ, mass spectrometry, and immunofluorescence imaging for the characterization of newly formed epidermis, following an initial acute wound for the first 96 h of epithelization. In particular, TOF-SIMS and confirmatory MALDI FT-ICR MS (/MS) analysis permitted the mapping of several lipid classes, including phospholipids, neutral lipids, cholesterol, ceramides, and free fatty acids. Endogenous lipid species were localized in discrete epidermal skin layers, including the stratum corneum (SC), stratum granulosum (SG), stratum basale (SB), and dermis. Experiments revealed that healthy re-epithelializing skin is characterized by diminished cholesterol sulfate signal along the stratum corneum toward the migrating epithelial tongue. The spatial distribution and relative abundances of cholesterol sulfate are reported and correlated with the healing time. The multimodal imaging approach enabled in situ high-confidence chemical mapping based on accurate mass and fragmentation pattern of molecular components. The use of postanalysis immunofluorescence imaging from the same tissue confirmed the localization of endogenous lipid species and Filaggrin and Cav-1 proteins at high spatial resolution (approximately a few microns).

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confidence: 99%

Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing

et al. 2019

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“…Due to the distinct differences in metabolic signature between and M1 and M2 macrophages, metabolic reprogramming is of vital significance, and disturbances in this metabolic reprogramming may affect the proper transition of macrophage subtype ( Mills and O'Neill, 2016 ). Studies have also provided evidence that there is a specific composition of lipids required in the inflammatory stage ( Wijesinghe et al, 2014 ; Wijesinghe et al, 2016 ; Yasukawa et al, 2020 ), and so it could be hypothesised that fatty acid oxidation plays an important role in wound healing.…”

Section: Metabolism In Wound Healingmentioning

confidence: 99%

Multifaceted roles of mitochondria in wound healing and chronic wound pathogenesis

Hunt,

Torres,

Bachar-Wikström

et al. 2023

Front. Cell Dev. Biol.

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Mitochondria are intracellular organelles that play a critical role in numerous cellular processes including the regulation of metabolism, cellular stress response, and cell fate. Mitochondria themselves are subject to well-orchestrated regulation in order to maintain organelle and cellular homeostasis. Wound healing is a multifactorial process that involves the stringent regulation of several cell types and cellular processes. In the event of dysregulated wound healing, hard-to-heal chronic wounds form and can place a significant burden on healthcare systems. Importantly, treatment options remain limited owing to the multifactorial nature of chronic wound pathogenesis. One area that has received more attention in recent years is the role of mitochondria in wound healing. With regards to this, current literature has demonstrated an important role for mitochondria in several areas of wound healing and chronic wound pathogenesis including metabolism, apoptosis, and redox signalling. Additionally, the influence of mitochondrial dynamics and mitophagy has also been investigated. However, few studies have utilised patient tissue when studying mitochondria in wound healing, instead using various animal models. In this review we dissect the current knowledge of the role of mitochondria in wound healing and discuss how future research can potentially aid in the progression of wound healing research.

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“…Lipid metabolism plays an important role in the wound-healing process. Several lipid regulators are implicated in several stages of wound healing, including inflammation, proliferation and tissue remodelling [31]. Lipid-based signalling controls various cellular functions during wound healing, including intercellular communication and tissue regeneration [32].…”

Section: Biological Activity Predictionmentioning

confidence: 99%

Incision-Wound Healing Activity of Sunflower Seed Oil (Helianthus Annuus L.: In Vivo and in Silico Study

MALIZA

2023

FARMACIA

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Sunflower seed oil (SFO) (Helianthus annuus L.) is rich in oleic acid and unsaturated fatty acids and can potentially treat various ailments. To validate the ethno-therapeutic claims of the SFO in skin diseases, the incision wound healing activity was studied. The objectives of this study were to evaluate the chemical content and incision wound-healing activities of SFO. Twenty-seven adult male Wistar rats were used, and a 2 cm length and 2 mm depth incision was made on the back of the rat. The rats were administered SFO, povidone iodine (PI), or were left untreated as a control group. Histological evaluations were conducted on postoperative wound tissue biopsies on days 0, 5 and 10. The SFO compounds were assessed using GC/MS analysis, and a subsequent in silico investigation was conducted based on the GC/MS findings. The binding affinity of these compounds to TNF-α, VEGFR1, IGFR1 and TGF-β1 was evaluated. On day 10, sunflower seed oil topical application accelerated wound healing by decreasing wound area and increasing wound contraction compared to untreated and PI-treated wounds. Moreover, granulation tissue expanded more, and the epidermis fully recovered. Twenty-four active compounds were detected using GC/MS, and six are responsible for lipid metabolism regulation and anti-inflammatory activity. The in silico study showed that 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol (3ß,5Z,7E) had a high affinity of -8.5 kcal/mol for TNF-α. The conclusion of this study shows that SFO has incision wound healing activity in vivo and in silico by controlling the inflammatory phase and proangiogenic factors with a high affinity to bind to TNF-α in the skin tissue. RezumatUleiul din semințe de floarea-soarelui (Helianthus annuus L.) (SFO) este bogat în acid oleic și în acizi grași nesaturați și prezintă potențialul terapeutic. Obiectivele acestui studiu au fost de a evalua conținutul chimic și efectul cicatrizant al SFO asupra rănilor provocate prin incizie. Au fost utilizați 27 de șobolani Wistar masculi adulți, iar pe spatele fiecărui animal s-a realizat o incizie de 2 cm lungime și 2 mm adâncime. Șobolanilor li s-a administrat SFO, iod-povidonă (PI) sau au fost lăsați netratați. S-au efectuat evaluări histologice pe biopsiile postoperatorii ale țesutului plăgii în zilele 0, 5 și 10. Compușii prezenți în SFO au fost evaluați cu ajutorul analizei GC/MS, iar pe baza rezultatelor s-a efectuat o investigație in silico. Astfel, a fost evaluată afinitatea de legare a acestor compuși pentru TNF-α, VEGFR1, IGFR1 și TGF-β1. În ziua 10, aplicarea topică a SFO a accelerat vindecarea prin scăderea diametrului rănilor și creșterea contracției în comparație cu rănile netratate sau tratate cu PI. Mai mult, țesutul de granulație s-a extins mai mult, iar epiderma s-a recuperat complet. Un număr de 24 de compuși activi au fost detectați cu ajutorul GC/MS, iar 6 dintre ei sunt responsabili de modularea metabolismului lipidic și de activitatea antiinflamatoare. Studiul in silico a arătat că 9,10-secocholesta-5,7,10(19)-trien-3,24,25-tri...

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Human as the Ultimate Wound Healing Model: Strategies for Studies Investigating the Dermal Lipidome

Cited by 8 publications

References 77 publications

Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing

Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing

Multifaceted roles of mitochondria in wound healing and chronic wound pathogenesis

Incision-Wound Healing Activity of Sunflower Seed Oil (Helianthus Annuus L.: In Vivo and in Silico Study

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