Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films

Ishaug-Riley, Susan L.; Okun, Laura E; Prado, G.N.; Applegate, Mark A.; Ratcliffe, Anthony

doi:10.1016/s0142-9612(99)00155-6

Cited by 225 publications

(147 citation statements)

References 47 publications

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“…While, the average WCA of a droplet of positioned on PP-Cl coated substrate was in the range of 90±1°t hat was in good agreement with the literature values [66][67][68]. By introduction of either hydrophilic PCL-alkyne (determined as 65±1.5°) [69][70][71], or PEG-alkyne (determined as 42±1.9°) units on the PP-Cl backbone, the WCA values of corresponding graft copolymers were decreased down to 83±2.1° and 78±1.8°, respectively. The difference observed in the graft copolymers could be explained by the interaction of the polymer surface chains with the water.…”

Section: Resultssupporting

confidence: 88%

Polypropylene-based graft copolymers via CuAAC click chemistry

Açık¹,

Sey²,

Taşdelen³

2018

Express Polym. Lett.

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Abstract. Graft copolymers from commercial chlorinated polypropylene (PP-Cl) possessing either poly(ethylene glycol) (PEG) or poly(ɛ-caprolactone) (PCL) grafts are synthesized by copper (I)-catalyzed azide-alkyne cycloaddition 'click' reaction (CuAAC). For this purpose, azido-functional polypropylene is prepared by nucleophilic substitution of chlorine groups of PP-Cl with azidotrimethylsilane-tetrabutylammonium fluoride. Whereas, the clickable alkyne end-functional PEG and PCL are independently synthesized by esterification reaction of poly(ethylene glycol) methyl ether with 4-pentyonic acid at room temperature and ring-opening polymerization of ε-caprolactone using stannous octoate as catalyst and propargyl alcohol as initiator. Finally, the corresponding graft copolymers, PP-g-PEG and PP-g-PCL, with different surface properties were successfully synthesized by CuAAC 'click' reaction under mild condition. Spectral, chromatographic and thermal analyses at various stages prove the formation of desired polypropylene-based graft copolymers with well-defined properties. Furthermore, the water contact angle values of PP-Cl, PP-g-PEG and PP-g-PCL are found as 90±1°, 78±1.8° and 83±2.1°, respectively.

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Section: Resultssupporting

confidence: 88%

Polypropylene-based graft copolymers via CuAAC click chemistry

Açık¹,

Sey²,

Taşdelen³

2018

Express Polym. Lett.

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“…There is, however, conflicting data in the literature regarding whether modified biodegradable polyesters can encourage cell adhesion and cell growth. 6,25 The aim of this study was to examine whether the surfaces of polyesters need to be modified to improve protein adhesion. Three polymers, poly(ethylene terephthalate) (PET), PLA, and PGA were selected for the study on the basis of the reactivity of their ester group.…”

Section: Introductionmentioning

confidence: 99%

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Atthoff¹,

Hilborn²

2006

J Biomed Mater Res

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Surface hydrolysis of polyester scaffolds is a convenient technique suggested to promote protein adsorption for improving cell attachment. We have, therefore, investigated the effect of hydrolysis of polyester surfaces for protein adsorption to clarify the conditions needed. Three polyesters, poly(ethylene terephthalate) (PET), poly(lactic acid) (PLA), and poly(glycolic acid) (PGA), were selected. Adsorption was investigated by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and quartz crystal microbalance (QCM). Hydrolyzed PET adsorbed significantly more proteins than nonhydrolyzed. Degradable polymers adsorbed at higher rates when the polymers were hydrolyzed prior to adsorption, but the same amount as nonhydrolyzed, suggesting spontaneous hydrolysis during the adsorption. XPS shows that hydrolysis prior to absorption for PET results in a surface nitrogen composition of ϳ14%, similar to pure protein (16%). Nonhydrolyzed PET surfaces showed only ϳ7% nitrogen, indicating protein layers thinner than ϳ10 nm. Adsorption to PLA and PGA shows nitrogen contents of 14 -15% in both cases. SEM revealed striking differences in morphology of the protein coating. Hydrolyzed or spontaneously hydrolyzable surfaces display a pronounced fibrous structure while nonhydrolyzed surfaces give smooth structures. In combination, the results show that surface hydrolysis increase adsorption rate, but not the amount of proteins on polyesters that degrades in vivo. Surface treatment of nondegradable polyester increases the total amount of proteins and induces the formation of fibrous protein structures. Post hydrolysis treatment by acetic acid, replacing the counter-ion to a proton, further enhances protein attachment. Finally, cell attachment experiments verifies that protein adsorption increase the cell attachment to polyester surfaces.

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“…In addition, no variations in cell spreading were observed between the different biomaterials. Although the cells studied adhered less to the PLLA membranes when compared to PLGA, their proliferative capacity was better when grown on the PLLA membrane [59]. In addition, the production of cartilaginous matrix and type II collagen was found to be lower for human chondrocytes www.intechopen.com…”

Section: Bioresorbable Devices For Cartilage Engineeringmentioning

confidence: 91%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (PLA), such as poly(L-lactic acid), poly(Dlactic acid) and poly(DL-lactic acid), as well as poly(glycolic acid) and polycaprolactone, have been extensively studied. These polymers are well known for their good biocompatibility, with their degradation products being eliminated from the body by metabolic pathways. Many reports have shown that the different PLA-based substrates do not present toxicity since the cells were found to differentiate over the different polymers, as demonstrated by the production of extracellular matrix components by various cell types. In this chapter, we describe the use of -hydroxy acids, highlighting the different forms of PLA scaffolds used as cell culture substrates and their applications in clinical practice. The chapter is divided into (1) Introduction; (2) Bioresorbable devices as cell culture substrates; (3) Cell adhesion to polymer substrates; (4) Tissue engineering and bioresorbable polymers; (5) Cell growth and proliferation on bioresorbable polymers; (6) Bioresorbable polymers for cartilage engineering; (7) Bioresorbable polymers for bone tissue engineering; (8) Bioresorbable polymers for skin tissue engineering, and (9) Conclusion.

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Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films

Cited by 225 publications

References 47 publications

Polypropylene-based graft copolymers via CuAAC click chemistry

Polypropylene-based graft copolymers via CuAAC click chemistry

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Bioresorbable Polymers for Tissue Engineering

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