Human Apolipoprotein C-II Quantitation by Sandwich Enzyme-Linked Immunosorbent Assay

Bury, J; Michiels, G; Rosseneu, Maryvonne

doi:10.1515/cclm.1986.24.7.457

Cited by 15 publications

(12 citation statements)

References 23 publications

(38 reference statements)

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“…38 Purified rabbit anti-apo C-II polyclonal antibody (10 g/mL) was used as the capture antibody. The same antibody conjugated to horseradish peroxidase was used for detection with a chromogen (o-phenylenediamine dihydrochloride; Sigma Chemical Co).…”

Section: Apo C-ii and Apo C-iii Elisasmentioning

confidence: 99%

Developmental and Pharmacological Regulation of Apolipoprotein C-II Gene Expression

Andersson

Majd

Lefebvre

et al. 1999

ATVB

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Abstract-Increased plasma triglyceride concentrations are often observed in metabolic disorders predisposing to coronary heart disease. Among the major determinants of plasma triglyceride metabolism are the apolipoproteins (apos) of the C class, C-I, C-II, and C-III. Whereas physiological concentrations of apo C-II are required for lipolysis of triglycerides by lipoprotein lipase (LPL), overexpression of all 3 C apolipoproteins leads to hypertriglyceridemia. In the present study, we investigated apo C-II gene regulation under conditions associated with profound changes in plasma triglyceride metabolism, ie, during postnatal development and after treatment with the triglyceride-lowering fibrate drugs, and compared its expression to that of apo C-I and apo C-III. Whereas the expression of both apo C-I and apo C-III is low in fetal liver, increases gradually after birth, and attains maximal levels after weaning, apo C-II gene expression is already detectable in the fetal liver, increases rapidly immediately after birth, and remains elevated throughout suckling. Thus, the increased ingestion of lipids during suckling is met by an earlier induction of apo C-II, the obligatory activator for LPL, compared with apo C-III and apo C-I, which antagonize triglyceride catabolism. Treatment of rats with fibrates decreased apo C-II gene expression in the liver, but not in the intestine, whereas apo C-I gene expression did not change. The decrease of liver apo C-II mRNA levels after fenofibrate occurred in a time-and dose-dependent manner and was reversible but appeared less pronounced than the decrease of apo C-III mRNA. Apo C-II mRNA levels were not affected after treatment with BRL49653, a peroxisome proliferator-activated receptor (PPAR)␥-specific ligand, suggesting that fibrates act on apo C-II expression via PPAR␣. Addition of fenofibric acid to primary rat and human hepatocytes resulted in a decrease of apo C-II expression. In conclusion, fibrates decrease gene expression of apo C-II and apo C-III, but not apo C-I, in rat and human hepatocytes. This decrease of apo C-II and apo C-III gene expression, together with a lowered apo C-III to apo C-II ratio, should result in an improved clearance of triglyceride-rich remnant lipoproteins from plasma, without hampering triglyceride lipolysis by LPL.

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Section: Apo C-ii and Apo C-iii Elisasmentioning

confidence: 99%

Developmental and Pharmacological Regulation of Apolipoprotein C-II Gene Expression

Andersson

Majd

Lefebvre

et al. 1999

ATVB

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“…13 In addition, wide variations in imrnunoassays can also be due to the use of either monoclonal or polyclonal antibodies and the species from which these are obtained.P…”

Section: Discussionmentioning

confidence: 99%

Indirect Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for Plasma Apolipoprotein E

et al. 1996

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SUMMARY.We have developed an indirect sandwich ELISA for measuring plasma apolipoprotein E (apo-E), using commercially available antibodies. A monoclonal anti-apo-E was used as the capture antibody and the captured apo-E detected with polyclonal anti-apo-E antiserum (goat). The detecting antibody was quantitated using horseradish peroxidase-conjugated rabbit immunoglobulin to goat immunoglobulins. There was no detectable cross-reactivity between the three antisera. Interference with the assay by apolipoprotein A-I, bilirubin and haemoglobin was not significant up to 1·7 giL, 1250 umol/L and 13·0 g/dL, respectively. The ELISA method showed high correlation with an established immunonephelometric method (ELISA apo-E= 1·04 Immunonephelometric apo-E+ 16; r 2=0'954, P

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“…Apo C-I levels were quantifi ed from frozen Oji-Cree serum samples by sandwich ELISA as described elsewhere ( 34 ). Briefl y, polyclonal rabbit anti-human apo C-I antibody (Academy Biomedical Co., Houston, TX) was coated overnight at 7°C onto 96-well polystyrene plates (Nunc-Immuno MaxiSorp, NUNC, Rochester, NY) at a dilution of 1:200, followed by one wash with 300 µL PBS per well.…”

Section: Quantitation Of Serum Apo C-i Concentrationmentioning

confidence: 99%

APOC1 T45S polymorphism is associated with reduced obesity indices and lower plasma concentrations of leptin and apolipoprotein C-I in aboriginal Canadians

Lahiry

Cao

Ban

et al. 2010

Journal of Lipid Research

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Human Apolipoprotein C-II Quantitation by Sandwich Enzyme-Linked Immunosorbent Assay

Cited by 15 publications

References 23 publications

Developmental and Pharmacological Regulation of Apolipoprotein C-II Gene Expression

Developmental and Pharmacological Regulation of Apolipoprotein C-II Gene Expression

Indirect Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for Plasma Apolipoprotein E

APOC1 T45S polymorphism is associated with reduced obesity indices and lower plasma concentrations of leptin and apolipoprotein C-I in aboriginal Canadians

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