Human and animal serotypes of Bacteroides gingivalis defined by crossed immunoelectrophoresis

Parent, Romain; Mouton, Christian; Lamonde, L; Bouchard, David

doi:10.1128/iai.51.3.909-918.1986

Cited by 67 publications

(44 citation statements)

References 33 publications

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“…Our understanding of the role that P. gingivalis plays in periodontal diseases, in root canal infections, and other oral infections would be furthered by ecologic and epidemiologic studies, and by studies of possible transmission from individual to individual. Biotyping and serotyping do not discriminate independent isolates very well since only 2 biotypes and only 3 serotypes have been reported (Laliberte & Mayrand 1983, Parent et al 1986, Fisher et al 1987, Umemoto et al 1989). Hence, the probability of 2 unrelated isolates having identical biotypes and serotypes is high.…”

Section: Porphyromonas Gingivalismentioning

confidence: 99%

The use of genomic DMA fingerprinting in studies of the epidemiology of bacteria in periodontitis

Genco

Loos

1991

J Clinic Periodontology

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Recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. Studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic DNA fingerprinting. Characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecular weight genomic DNA by restriction endonucleases. It is assumed that each DNA fingerprint represents a clonal type. In this report, we review and analyze studies of the epidemiology of bacteria associated with orofacial infections with an emphasis on periodontal disease. Studies of nontypable (NT) Haemophilus influenzae associated with recurrent otitis media illustrate the utility of this technique. DNA fingerprinting clearly demonstrates genetic heterogeneity of NT H. influenzae isolates, and clonality of infection of any individual. Furthermore, DNA fingerprinting has shown that the same clonal type is seen in siblings concurrently suffering from otitis media, suggesting horizontal transmission within the family. Studies of mutans Streptococci also show extensive genetic heterogeneity and show vertical transmission of a predominant clonal type only from mother to infant, but not from father to infant. Studies of Actinobacillus actinomycetemcomitans show considerable genetic heterogeneity among monkey isolates. Thus far, three clonal types have been reported with DNA fingerprinting among isolates from periodontal patients, but additional genetic heterogeneity can be found using specific DNA fragments as probes in hybridization experiments. Intrafamilial transmission of A. actinomycetemcomitans has been demonstrated. Porphyromonas (Bacteroides) gingivalis shows extensive genetic heterogeneity and case reports suggest clonal infection of any one individual. In contrast, results with DNA fingerprinting of Eikenella corrodens, Fusobacterium nucleatum, and Bacteroides intermedius show that individuals may be infected with 2 or more clonal types. These studies point to the great potential of DNA fingerprinting for investigating the epidemiology of putative orofacial pathogens. Such studies with periodontal microorganisms will likely reveal steps in the acquisition, intraoral and person-to-person transmission, which then could possibly be inhibited or interfered with to prevent periodontal disease or its recurrence.

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Section: Porphyromonas Gingivalismentioning

confidence: 99%

The use of genomic DMA fingerprinting in studies of the epidemiology of bacteria in periodontitis

Genco

Loos

1991

J Clinic Periodontology

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“…Glass bead-EDTA (GBE) extract. An BDTA cell-surface extract was prepared by the method of Parent et al (24), except that the preliminary washes were omitted. Harvested cells from an early stationary-phase culture were resuspended directly in 50 mM Tris HCl, 5 mM EDTA, final pH 8.6, at a concentration of 3 g wet pellet per 35 ml of buffer; 5 ml of glass beads (0.15-0.2 mm diameter) was added, and the suspension shaken in an Erlenmeyer flask for 48 h at 4°C, on an orbital shaker at 120 rpm.…”

Section: Bacteriai Extractsmentioning

confidence: 99%

The hemagglutinating adhesin HA‐Ag2 of Bacteroides gingivalis is distinct from fimbrilin

Mouton

Eidhin

Deslauriers

et al. 1991

Oral Microbiology and Immunology

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We carried out a series of immunoblots with antigenic preparations from the periodontal pathogen Bacteroides gingivalis using antisera of restricted specificity for the hemagglutinating adhesin HA-Ag2, and for the major structural subunit of the fimbriae (fimbrilin). We have been able to show that these 2 antigens are distinct. The fimbrilin subunit had an apparent molecular weight of 42 kDa in all of the bacterial preparations tested. HA-Ag2 occurred as a pair of bands at 43 and 49 kDa in outer membranes prepared as extracellular vesicles, and at 33 and 38 kDa in glass-bead-EDTA extracted antigens and in sheared-cell outer membranes prepared in the presence of EDTA. No HA-Ag2 was found in an enriched fimbrial preparation. The 2 antigens could thus be distinguished on the basis of their behaviour when subjected to different extraction techniques. The lower apparent molecular weight of HA-Ag2 (a pair of bands at 33 and 38 kDa) was invariably associated with the presence of EDTA in the buffers used to prepare the extracts, and the effect could be partially prevented by adding MgCl2 to the extraction buffer. The difference in apparent molecular weight of HA-Ag2 in the different extracts can thus be attributed either to an EDTA-sensitive tertiary conformation of its component polypeptides, or to an EDTA-sensitive linkage of each of these polypeptides to an unknown component of approximately 10 kDa.

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“…Little is known about the taxonomic status of these isolates, which probably belong to different and undescribed species. Recently the asaccharolytic, catalase positive, black-pigmented Bacieroides isolated from beagle dogs (Laliberte & Mayrand 1983, Mikx et al 1984 were described as a biotype of B. gingivalis (Parent et al 1986).…”

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confidence: 99%

The role of black‐pigmented Bacteroides in human oral infections

Winkelhoff

Steenbergen

Graaff

1988

J Clinic Periodontology

295

164

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Today, 10 black‐pigmented Bacteroides (BPB) species are recognized. The majority of these species can be isolated from the oral cavity. BPB species are involved in anaerobic infections of oral and non‐oral sites. In the oral cavity. BPB species are associated with gingivitis, periodontitis, endodontal infections and odontogenic abscesses. Cultural studies suggest a specific role of the various BPB species in the different types of infection. Bacteroides gingivalis is closely correlated with destructive periodontitis in adults as well as in juveniles. Bacteroides intermedius seems to be less specific since it is found in gingivitis, periodontitis, endodontal infections and odontogenic abscesses. The recently described Bacteroides endodontalis is closely associated with endodontal infections and odontogenic abscesses of endodontal origin. There are indications that these periodontopathic BPB species are only present in the oral cavity of subjects suffering from periodontal breakdown, being absent on the mucosal surfaces of subjects without periodontal breakdown. BPB species associated with healthy oral conditions are Bacteroides melaninogenicus, Bacteroides denticola and Bacteroides loescheii. There are indications that these BPB species are part of the normal indigenous oral microflora. Many studies in the past have documented the pathogenic potential and virulence of BPB species. This virulence can be explained by the large numbers of virulence factors demonstrated in this group of microorganisms. Among others, the proteolytic activity seems to be one of the most important features. Several artificial substrates as well as numerous biological proteins are degraded. These include anti‐inflammatory proteins such as alpha‐2‐macroglobulin, alpha‐1‐antitrypsin, C3 and C5 complement factors and immunoglobulins. B. gingivalis is by far the most proteolytic species, followed by B. endodontalis. Like other bacteria, the lipopolysaccharide of B. gingivalis has shown to be active in bone resorption in vitro and is capable in stimulating interleukin‐1 production in human peripheral monocytes. Based on the well documented association with periodontal disease and the possession of relevant virulence factors. BPB species must be considered as important micro‐organisms in the etiology of oral infections. B. gingivalis seems to be the most pathogenic and virulent species.

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Human and animal serotypes of Bacteroides gingivalis defined by crossed immunoelectrophoresis

Cited by 67 publications

References 33 publications

The use of genomic DMA fingerprinting in studies of the epidemiology of bacteria in periodontitis

The use of genomic DMA fingerprinting in studies of the epidemiology of bacteria in periodontitis

The hemagglutinating adhesin HA‐Ag2 of Bacteroides gingivalis is distinct from fimbrilin

The role of black‐pigmented Bacteroides in human oral infections

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