Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Magaña‐Guerrero, Fátima Sofía; Domínguez-López, Alfredo; Martinez-Aboytes, Pamela; Buentello-Volante, Beatríz; Garfias, Yonathan

doi:10.1038/s41598-017-10962-2

Cited by 42 publications

(41 citation statements)

References 72 publications

(72 reference statements)

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“…All donors included in this study were 18 years old or older, and the criteria for donation were similar to those reported by Chávez‐García et al . The cells were obtained as previously described, with slight modifications . Briefly, the chorion was separated from the amnion and discarded, while the amnion was incubated with trypsin–EDTA at 37°C to release the amniotic epithelial cells.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

Navas

Magaña‐Guerrero

Domínguez-López

et al. 2018

Stem Cells Translational Medicine

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115

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Acute ocular chemical burns are ophthalmic emergencies requiring immediate diagnosis and treatment as they may lead to permanent impairment of vision. The clinical manifestations of such burns are produced by exacerbated innate immune response via the infiltration of inflammatory cells and activation of stromal fibroblasts. New therapies are emerging that are dedicated to repair mechanisms that improve the ocular surface after damage; for example, transplantation of stem cells (SC) has been successfully reported for this purpose. The pursuit of easily accessible, noninvasive procedures to obtain SC has led researchers to focus on human tissues such as amniotic membrane. Human amniotic mesenchymal SC (hAM‐MSC) inhibits proinflammatory and fibrotic processes in different diseases. hAM‐MSC expresses low levels of classical MHC‐I and they do not express MHC‐II, making them suitable for regenerative medicine. The aim of this study was to evaluate the effect of intracameral injection of hAM‐MSC on the clinical manifestations, the infiltration of inflammatory cells, and the activation of stromal fibroblasts in a corneal alkali‐burn model. We also determined the in vitro effect of hAM‐MSC conditioned medium (CM) on α‐SMA+ human limbal myofibroblast (HLM) frequency and on release of neutrophil extracellular traps (NETs). Our results show that intracameral hAM‐MSC injection reduces neovascularization, opacity, stromal inflammatory cell infiltrate, and stromal α‐SMA+ cells in our model. Moreover, in in vitro assays, CM from hAM‐MSC decreased the quantity of α‐SMA+ HLM and the release of NETs. These results suggest that intracameral hAM‐MSC injection induces an anti‐inflammatory and anti‐fibrotic environment that promotes corneal wound healing. Stem Cells Translational Medicine 2018;7:906–917

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Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

Navas

Magaña‐Guerrero

Domínguez-López

et al. 2018

Stem Cells Translational Medicine

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115

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“…The mesenchymal stem cells obtained from mesoderm of human amniotic membrane possess immunosuppressive functions through soluble factors such as prostanoids and proteins [34]. AME, an extract of the human amniotic membrane, has also been shown to have anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lead on Human Middle Ear Epithelial Cells

Kim

Shin

et al. 2018

BioMed Research International

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Lead is a ubiquitous metal in the environment, but no studies have examined lead toxicity on the middle ear. Here, we investigated lead toxicity and its mechanism in human middle ear epithelial cells (HMEECs). Moreover, we investigated the protective effects of amniotic membrane extract (AME) and chorionic membrane extract (CME) against lead toxicity in HMEECs. Cell viability was analyzed using the cell counting kit, and reactive oxygen species (ROS) activity was measured using a cellular ROS detection kit. After lead(II) acetate trihydrate treatment, mRNA levels of various genes were assessed by semiquantitative real-time polymerase chain reaction. Following treatment with AME or CME after lead exposure, the changes in cell viability, ROS activity, and gene expression were analyzed. Exposure to >100 g/mL of lead(II) acetate trihydrate caused a significant decrease in cell viability and increased ROS production in HMEECs. Lead exposure significantly increased the mRNA expression of genes encoding inflammatory cytokines and mucins. Administration of AME or CME restored cell viability, reduced ROS activity, and ameliorated mRNA levels. Our findings suggest that environmental lead exposure is related to the development of otitis media, and AME and CME may have antioxidative and anti-inflammatory effects against lead toxicity.

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“…55,56 TSG-6 in conditioned medium from human AM-derived MSCs was also related to the inflammatory response using LPS-stimulated murine bone marrow-derived neutrophils. 57 Human corneal epithelial cell migration increased significantly with the use of 15% and 30% AMconditioned medium. 58 We observed that AMF-sup had similar effects to AM-sup in this study .…”

Section: Discussionmentioning

confidence: 96%

Effects of Amniotic Membrane–Derived Fibroblast Supernatant on Corneal Epithelium

Higa

Higuchi

Kimoto

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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Citation: Higa K, Higuchi J, Kimoto R, et al. Effects of amniotic membranederived fibroblast supernatant on corneal epithelium. Invest Ophthalmol Vis Sci. 2019;60:3718-3726. https:// doi.org/10.1167/iovs.19-27041 PURPOSE. To evaluate the effects of human amniotic membrane-derived fibroblast (AMF) cell supernatant (AMF-sup) on corneal epithelium.METHODS. The phenotype of AMF cells was analyzed by flow cytometry using cell-surface markers. AMF cells were also induced to form osteoblasts and neural cells, and cell phenotypes were observed by staining and RT-PCR. Cultivated human corneal limbal epithelial sheets generated using AMF-sup were analyzed using immunohistochemistry and colony-forming efficiency, and the wound healing of epithelial defects was observed using a tissue-punch method. The effects of instillation of each supernatant in a rabbit corneal epithelial wound healing model were compared. RESULTS.Mesenchymal stem cell (CD29, CD44, CD73, and CD90) and neural crest (CD49d and CD56) markers were expressed on the AMF cell surface. Following induction of differentiation, isolated AMF cells showed characteristics of osteoblasts and neural cells. Application of AMF-sup resulted in maintenance of the limbal epithelial phenotype and immature state, and significantly promoted wound healing in cultivated human corneal limbal epithelial sheets (P < 0.05) and rabbit corneal epithelium (P < 0.05) compared with the control.CONCLUSIONS. These data suggest that AMF cells have multi-differentiation potential, and that AMF-sup is effective in maintaining the limbal epithelial phenotype and promoting corneal epithelial wound healing, which may be of value in ocular surface reconstruction.

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Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6

Cited by 42 publications

References 72 publications

Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

Effect of Lead on Human Middle Ear Epithelial Cells

Effects of Amniotic Membrane–Derived Fibroblast Supernatant on Corneal Epithelium

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