Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules

Kim, Sun Hee; Bang, So Hee; Kang, So Yeong; Park, Ki Dae; Eom, Jun Ho; Oh, Il Ung; Yoo, Si Hyung; Kim, Chan Wha; Lee, Ji Hyun

doi:10.1016/j.tice.2014.10.003

Cited by 13 publications

(13 citation statements)

References 36 publications

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“…In line with our in vitro observations, others have shown that intra-tumor injection of hAMSCs inhibited growth and induced apoptosis of C6 glioma (Jiao et al, 2012). To the contrary, CM from hAMSCs was shown to promote the proliferation and migration of breast cell lines (Kim et al, 2015), lung adenocarcinoma cells (SPC-A-1), and gastric carcinoma cells (BGC-823) (Meng et al, 2019). Interestingly, the authors of this last study only partially confirmed their findings in vivo as hAMSCs were able to stimulate tumor growth when subcutaneously co-injected with SPC-A-1 or BGC-823 tumor cells, but not when injected intravenously 12 days after tumor cell injection when the tumor is well-established (Meng et al, 2019).…”

Section: Perinatal Msc As Therapeutic Strategy In Cancersupporting

confidence: 90%

The Multifaceted Roles of MSCs in the Tumor Microenvironment: Interactions With Immune Cells and Exploitation for Therapy

Papait

Stefani

Cargnoni

et al. 2020

Front. Cell Dev. Biol.

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The tumor microenvironment (TME) plays a critical role in tumorigenesis and is composed of different cellular components, including immune cells and mesenchymal stromal cells (MSCs). In this review, we will discuss MSCs in the TME setting and more specifically their interactions with immune cells and how they can both inhibit (immunosurveillance) and favor (immunoediting) tumor growth. We will also discuss how MSCs are used as a therapeutic strategy in cancer. Due to their unique immunomodulatory properties, MSCs isolated from perinatal tissues are intensely explored as therapeutic interventions in various inflammatory-based disorders with promising results. However, their therapeutic applications in cancer remain for the most part controversial and, importantly, the interactions between administered perinatal MSC and immune cells in the TME remain to be clearly defined.

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Section: Perinatal Msc As Therapeutic Strategy In Cancersupporting

confidence: 90%

The Multifaceted Roles of MSCs in the Tumor Microenvironment: Interactions With Immune Cells and Exploitation for Therapy

Papait

Stefani

Cargnoni

et al. 2020

Front. Cell Dev. Biol.

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“…In fact, there are very few reports, and these have divergent findings, on cancer cell interactions and behavior after dHACM exposure. 2,6,7 In the present study, the in vitro experiment showed that the use of dHACM allografts had a modest impact on tumor cell growth. Although the dHACM extract alone did not present as a significant cell stimulator compared with the other mediums, the combination of dHACM extract with 10%FBS resulted in accelerated tumor cell growth in both LNCaP and UMUC3 cell lines.…”

Section: Discussionsupporting

confidence: 44%

“…In fact, there are very few reports, and these have divergent findings, on cancer cell interactions and behavior after dHACM exposure. 2,6,7…”

Section: Discussionmentioning

confidence: 99%

The potential risk of tumor progression after use of dehydrated human amnion/chorion membrane allograft in a positive margin resection model

Alvim

Hughes

Somma

et al. 2019

Therapeutic Advances in Urology

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Objective: The objective of this study was to examine the impact of dehydrated human amnion/chorion membrane (dHACM) allografts on prostate and bladder cancer growth in the setting of residual disease and positive surgical margins. Materials and methods: A commercially available version of dHACM was used. Cytokines were identified and quantified, followed by comparative analysis of cell growth in two different human cell lines: prostate cancer (LNCaP) and bladder cancer (UM-UC-3), in vitro and in vivo. Tumor growth between the two groups, membrane versus no membrane implant, was compared and immunohistochemistry studies were conducted to quantify CD-31, Ki-67, and vimentin. A Student’s unpaired t -test was used to determine statistical significance. Results: The UM-UC-3 and LNCaP cells grew quicker in medium plus 10% serum and dHACM extract than in the other media ( p = 0.03). A total of 28 distinct cytokines were found in the extract, 11 of which had relatively high concentrations and are associated with prostate and bladder cancer tumor progression. In vivo LNCaP model, after 10 weeks, the median tumor volume in the membrane group was almost threefold larger than the partial resection alone ( p = 0.01). Two weeks after resection, in the UM-UC-3 model, the membrane group reached fourfold larger than the partial resection without membrane group ( p < 0.01). In both groups, the expression of CD-31 and Ki-67 markers were similar and showed no statistical significance ( p > 0.05). It was only in the LNCaP tumors that vimentin expression was significantly higher in the group without membrane compared with the membrane group ( p = 0.008). Conclusion: The use of dHACM after partial tumor resection is related to faster tumor relapse and growth in prostate and urothelial cancer in vivo models, showing a potential risk of rapid local recurrence in patients at high risk of positive margins.

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“… 13 Extensive research is focused on the AM as a potentially unlimited source of stromal cells that allow cell collection through simple, safe, non-invasive, and cost-effective methods compared to other stem cell sources such as bone marrow. 14 …”

Section: Introductionmentioning

confidence: 99%

Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy

Jafari

Rezaei‐Tavirani

Niknejad

et al. 2021

Technol Cancer Res Treat

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Objectives: Traditional breast cancer treatments have challenges including inefficiency, multidrug resistance, severe side effects, and targeting non-specifically. The development of alternative treatment strategies has attracted a great deal of interest. Using the amniotic membrane has become a promising and convenient new approach for cancer therapy. This study aimed to evaluate the anti-cancer ability of conditioned medium extracted from the human amniotic membrane (hAM-CM) on breast cancer cells. Methods: Conditioned medium was collected after 48 h incubation of hAM in epithelial up manner. MTT, cell cycle, apoptosis, colony formation, and sphere assays were used to determine the impact of hAM-CM on breast cancer cell lines. The effects of hAM-CM on the migration and invasion of breast cancer cells were determined using scratch wound healing and transwell assays, respectively. Results: Based on the results, cell viability was significantly decreased by hAM-CM in a dose-dependent manner. The hAM-CM remarkably induced apoptosis and necrosis of cancer cells. Moreover, cell migration and invasion potential of cancer cells decreased after the hAM-CM treatment. Further, both the number of colonies and their morphologies were affected by the treatment. In the treated group, a significant decrease in the number of colonies along with an obvious change in their morphologies from holoclone shape to a dominant paracolone structure was observed. Conclusion: Our results indicate that the conditioned medium derived from the human amniotic membrane able to inhibit proliferation and metastasis of tumor cells and can be considered a natural and valuable candidate for breast cancer therapy.

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Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules

Cited by 13 publications

References 36 publications

The Multifaceted Roles of MSCs in the Tumor Microenvironment: Interactions With Immune Cells and Exploitation for Therapy

The Multifaceted Roles of MSCs in the Tumor Microenvironment: Interactions With Immune Cells and Exploitation for Therapy

The potential risk of tumor progression after use of dehydrated human amnion/chorion membrane allograft in a positive margin resection model

Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy

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