Human Amniotic Membrane as Differentiating Matrix for
            <i>in Vitro</i>
            Chondrogenesis

Naseer, Nadia; Bashir, Saliha; Latief, Noreen; Latif, Farzana; Khan, Shaheen N.; Riazuddin, Sheikh

doi:10.2217/rme-2018-0017

Cited by 7 publications

(9 citation statements)

References 45 publications

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“…We analyzed the cytocompatibility of both, the epithelial and basal sides of D-hAM with hUC-MSC separately. Consistent with earlier studies, 23,25,61 hUC-MSC remained viable and proliferated actively on D-hAM for up to 15 days in culture. Moreover, the present study showed that hUC-MSC seeded on the basal side of D-hAM presented significantly higher proliferation on the basal surface than the epithelial surface, which is a relatively novel finding.…”

Section: Discussionsupporting

confidence: 90%

“…Moreover, several previous studies have shown that MSCs attach to and proliferate in the D-hAM. [23][24][25] However, the compatibility of the two surfaces of the D-hAM with MSCs have not been compared. Therefore, the objectives of this study were to investigate the effect of decellularization on the proteomic profile of decellularized hAM (D-hAM) prepared by a salt-based decellularization process using LC-MS/MS and determine the comparative compatibility of the two surfaces of D-hAM with human umbilical cord mesenchymal stem cells (hUC-MSC).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the proteome profile of decellularized human amniotic membrane and its biocompatibility with umbilical cord‐derived mesenchymal stem cells

Ahmed,

Tauseef,

Ainuddin

et al. 2024

J Biomedical Materials Res

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Extracellular matrix‐based bio‐scaffolds are useful for tissue engineering as they retain the unique structural, mechanical, and physiological microenvironment of the tissue thus facilitating cellular attachment and matrix activities. However, considering its potential, a comprehensive understanding of the protein profile remains elusive. Herein, we evaluate the impact of decellularization on the human amniotic membrane (hAM) based on its proteome profile, physicochemical features, as well as the attachment, viability, and proliferation of umbilical cord‐derived mesenchymal stem cells (hUC‐MSC). Proteome profiles of decellularized hAM (D‐hAM) were compared with hAM, and gene ontology (GO) enrichment analysis was performed. Proteomic data revealed that D‐hAM retained a total of 249 proteins, predominantly comprised of extracellular matrix proteins including collagens (collagen I, collagen IV, collagen VI, collagen VII, and collagen XII), proteoglycans (biglycan, decorin, lumican, mimecan, and versican), glycoproteins (dermatopontin, fibrinogen, fibrillin, laminin, and vitronectin), and growth factors including transforming growth factor beta (TGF‐β) and fibroblast growth factor (FGF) while eliminated most of the intracellular proteins. Scanning electron microscopy was used to analyze the epithelial and basal surfaces of D‐hAM. The D‐hAM displayed variability in fibril morphology and porosity as compared with hAM, showing loosely packed collagen fibers and prominent large pore areas on the basal side of D‐hAM. Both sides of D‐hAM supported the growth and proliferation of hUC‐MSC. Comparative investigations, however, demonstrated that the basal side of D‐hAM displayed higher hUC‐MSC proliferation than the epithelial side. These findings highlight the importance of understanding the micro‐environmental differences between the two sides of D‐hAM while optimizing cell‐based therapeutic applications.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Assessment of the proteome profile of decellularized human amniotic membrane and its biocompatibility with umbilical cord‐derived mesenchymal stem cells

Ahmed,

Tauseef,

Ainuddin

et al. 2024

J Biomedical Materials Res

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“…Immunocytochemistry was performed by the previously established protocol in stem cell lab 16 . CD73 (41–0200 Invitrogen, USA), CD90 (MA1‐35307 Invitrogen, USA) and CD105 (MA5‐17041 Invitrogen, USA) and negative markers CD34 (11–0341‐82 Invitrogen, USA), CD45 (MA1‐7654 Invitrogen, USA) were used in particular dilutions.…”

Section: Methodsmentioning

confidence: 99%

Sarcococca saligna fabricated gold nanoparticles alleviated in vitro oxidative stress and inflammation in human adipose‐derived stem cells

et al. 2023

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Oxidative stress is a destructive phenomenon that affects various cell structures including membranes, proteins, lipoproteins, lipids, and DNA. Oxidative stress and inflammation owing to lifestyle changes may lead to serious diseases such as Cancers, Gout, and Arthritis etc. These disorders can be prevented using different therapeutic strategies including nanomedicine. Biosynthesized gold nanoparticles (GNPs) because of their anti‐inflammatory and antioxidant bioactivities can be key player in reversal of these ailments. This study was carried out to evaluate the anti‐inflammatory and antioxidant potential of bio fabricated GNPs with Sarcococca saligna (S. saligna) extract on injured human adipose‐derived Mesenchymal stem cells (hADMSCs). GNPs were characterized by ultraviolet–visible (UV–Vis) spectroscopy, Scanning Electron Microscopy (SEM), x‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and energy dispersive x‐ray (EDS). Phytochemical screening of biosynthesized GNPs exhibited a significant release of polyphenols, that is, total phenolic content (TPC) and total flavonoid content (TFC). GNPs priming amended the in vitro injury caused by Monosodium Iodoacetate (MIA) as exhibited by improved cell viability, wound closure response and superoxide dismutase activity (SOD). The anti‐inflammatory conduct assessed through NF‐κB pathway and other associated inflammatory markers reported down‐regulation of TNF‐α (0.644 ± 0.045), IL‐1β (0.694 ± 0.147) and IL‐6 (0.622 ± 0.112), apoptosis causing genes like Caspase‐3 (0.734 ± 0.13) and BAX (0.830 ± 0.12), NF‐κB pathway, p65 (0.672 ± 0.084) and p105 (0.539 ± 0.083) associated genes. High SOD activity (95 ± 5.25%) revealed by treated hADMSCs with GNPs also supported the antioxidant role of GNPs in vitro model. This study concludes that S. saligna bio fabricated GNPs priming may improve the therapeutic potential of hADMSCs against chronic inflammatory problems by regulating NF‐κB pathway.

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“…reported the potential of decellularized AM basement membrane to support proliferation and differentiation of umbilical cord-derived MSCs (UC-MSCs) and placenta-derived MSCs (P-MSCs) into chondrocyte-like cells [49]. Khorramirouz et al also demonstrated the ability of decellularized AM to support AT-MSCs differentiation into cardiomyocytes when applied as a cardiac patch for the treatment of acute myocardial infarction in rat models [50].…”

Section: Amniotic Membranementioning

confidence: 99%