Human amniotic fluid modulation of collagenase production in cultured fibroblasts

Vadillo-Ortega, Felipe; González-Ávila, Georgina; Villaneuva-Diaz, Carlos; Banales, José Luis; Selman-Lama, M; Durán, A.

doi:10.1016/s0002-9378(11)80043-8

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…Nonterm amniotic fluids however failed to do the same. This phenomenon was therefore proposed as a model for studying the collagen degradation of fetal membranes during term gestation ( 2 ). Separate cell culture from different layers of fetal membranes and culture of purified placental trophoblast cells showed that placental syncytiotrophoblast and amnion epithelial cells exclusively produced matrix metalloproteinase-9 (MMP-9); chorion trophoblast cells secreted both MMP-2 and MMP-9, but amnion mesenchymal cells produced only MMP-2.…”

mentioning

confidence: 99%

The Role of Epithelial to Mesenchymal Transition in Human Amniotic Membrane Rupture

Janzen

Sen

Lei

et al. 2016

The Journal of Clinical Endocrinology & Metabolism

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Context:Biochemical weakening of the amnion is a major factor preceding preterm premature rupture of membranes (PPROMs), leading to preterm birth. Activation of matrix metalloproteinases (MMPs) is known to play a key role in collagen degradation of the amnion; however, epithelial to mesenchymal transition (EMT) that is also induced by MMP activation has not been investigated as a mechanism for amnion weakening.Objective:To measure amniotic EMT associated with vaginal delivery (VD) compared with unlabored cesarean sections (CSs), and to assess changes in amniotic mechanical strength with pharmacologic inhibitors and inducers of EMT, thus testing the hypothesis that EMT is a key biochemical event that promotes amniotic rupture.Findings:(1) Amnions taken from VD contained a significantly increased number of mesenchymal cells relative to epithelial cells compared with unlabored CS by fluorescence-activated cell sorting analysis (60% vs 10%); (2) tumor necrosis factor (TNF)–α stimulation of amniotic epithelial cells increased expression of the mesenchymal marker vimentin after 2 days; (3) EMT inhibitor, etodolac, significantly increased the time and mechanical pressure required to rupture the amnion; and (4) TNF-α and another pharmacologic EMT inducer, ethacridine, decreased the time and mechanical pressure required for amnion rupture, further confirming that the mesenchymal phenotype significantly weakens the amnion.Conclusions:This work demonstrated amniotic cell EMT was associated with labor and EMT decreased the tensile strength of the amnion. These findings suggest a role for EMT in the pathophysiology of PPROM and may provide a basis for development of therapies to prevent preterm labor.

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mentioning

confidence: 99%

The Role of Epithelial to Mesenchymal Transition in Human Amniotic Membrane Rupture

Janzen

Sen

Lei

et al. 2016

The Journal of Clinical Endocrinology & Metabolism

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“…This implies that something in addition to the inflammatory response to pathogen exposure induces changes in MMP2 concentration. Other studies have consistently indicated that pro‐inflammatory cytokines or GBS infection do not increase the expression of active MMP2 or 9 36 and, in some cases, may down‐regulate its expression and activity in cytotrophoblast 37–39 …”

Section: Resultsmentioning

confidence: 96%

“…Other studies have consistently indicated that pro-inflammatory cytokines or GBS infection do not increase the expression of active MMP2 or 9 36 and, in some cases, may down-regulate its expression and activity in cytotrophoblast. [37][38][39] Studies performed by Zaga-Clavelina, et al 40 in transwell devices made from human fetal membranes found an increase in pro-MMP2…”

Section: Gelatinases (Mmps 2 9)mentioning

confidence: 99%

Matrix metalloproteinases in preterm prelabor rupture of membranes in the setting of chorioamnionitis: A scoping review

Nguyen

Aronoff

Eastman

2022

American J Rep Immunol

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Fetal or gestational membranes extend from the placenta to enclose the fetus and amniotic fluid. While the membranes spontaneously rupture at term in normal pregnancies, they can rupture prematurely before the onset of labor, termed preterm prelabor rupture of membranes (PPROM). PPROM can be triggered by bacterial infection or sterile inflammation in the membranes, known as chorioamnionitis (CAM). The membranes derive their tensile strength from a collagen‐rich extracellular matrix (ECM); as such, understanding the enzymes and processes that can degrade the membrane ECM are of paramount importance. Matrix metalloproteinases (MMPs) are a class of enzymes capable of degrading collagen and other components of the ECM, and can be induced by inflammation. We used a scoping review to address the question of how MMP activity is associated with PPROM, particularly their induction due to sterile or nonsterile CAM. We have found that the most studied MMPs in PPROM were MMPs 2, 8, and 9. Additionally, some MMPs are constitutively active, while others are induced by inflammation. Mechanistic studies of the pathways that induce MMP activation are sparse, and this area is ripe for future studies. Targeting MMP activation could be a future strategy to delay or prevent PPROM.

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“…33 Vadillo-Ortega et al showed that increased activity of the collagenolytic enzyme matrix metalloproteinase 9 in the amniotic fluid is closely linked to the induction of labor. 18 Therefore, the chorioamniotic membranes of neonates delivered by primary CS exhibit greater extensibility and tensile strength compared with those of neonates delivered by vaginal deliveries. 33 As the amniotic fluid is swallowed by the neonate and flows into the nasal cavity, it will also contact HM.…”

Section: Role Of Enzymesmentioning

confidence: 99%

“…17 Further, labor induces the activity of collagenolytic enzymes in the amniotic fluid. 18 These mechanical and enzymatic alterations might also influence the lacrimal drainage system.…”

mentioning

confidence: 99%

Association between Congenital Nasolacrimal Duct Obstruction and Delivery by Cesarean Section

et al. 2014

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Human amniotic fluid modulation of collagenase production in cultured fibroblasts

Cited by 14 publications

References 23 publications

The Role of Epithelial to Mesenchymal Transition in Human Amniotic Membrane Rupture

The Role of Epithelial to Mesenchymal Transition in Human Amniotic Membrane Rupture

Matrix metalloproteinases in preterm prelabor rupture of membranes in the setting of chorioamnionitis: A scoping review

Association between Congenital Nasolacrimal Duct Obstruction and Delivery by Cesarean Section

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