Human Amniotic Fluid Mesenchymal Stem Cell-Derived Exosomes Inhibit Apoptosis in Ovarian Granulosa Cell via miR-369-3p/YAF2/PDCD5/p53 Pathway

Geng, Zixiang; Chen, Haiyang; Zou, Gang; Yuan, Long; Liu, Peng; Li, Bingrong; Zhang, Kaiyong; Jing, Fangyuan; Nie, Xiaoli; Liu, Te; Zhang, Bimeng

doi:10.1155/2022/3695848

Cited by 29 publications

(18 citation statements)

References 43 publications

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“…In the apoptotic signaling network, the p53 signaling pathway regulates the expression of pro-apoptotic genes, including Bax, Bak, Bad, and Apaf-1. However, miR-369-3p, miR-644-5p, and miR-125b-5p in MSCs-Exos exert antiapoptotic effects by inhibiting the activation of p53 [ 32 , 55 – 57 ]. And interestingly, miR-455-3p and miR-19a exhibit antiapoptotic effects by inhibiting JNK and subsequent activation of p53 and caspase-3 [ 58 , 59 ].…”

Section: Mscs Protect Cells From Apoptosismentioning

confidence: 99%

The dual role of mesenchymal stem cells in apoptosis regulation

Chen,

Xia,

Yao

et al. 2024

Cell Death Dis

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Mesenchymal stem cells (MSCs) are widely distributed pluripotent stem cells with powerful immunomodulatory capacity. MSCs transplantation therapy (MSCT) is widely used in the fields of tissue regeneration and repair, and treatment of inflammatory diseases. Apoptosis is an important way for tissues to maintain cell renewal, but it also plays an important role in various diseases. And many studies have shown that MSCs improves the diseases by regulating cell apoptosis. The regulation of MSCs on apoptosis is double-sided. On the one hand, MSCs significantly inhibit the apoptosis of diseased cells. On the other hand, MSCs also promote the apoptosis of tumor cells and excessive immune cells. Furthermore, MSCs regulate apoptosis through multiple molecules and pathways, including three classical apoptotic signaling pathways and other pathways. In this review, we summarize the current evidence on the regulation of apoptosis by MSCs.

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Section: Mscs Protect Cells From Apoptosismentioning

confidence: 99%

The dual role of mesenchymal stem cells in apoptosis regulation

Chen,

Xia,

Yao

et al. 2024

Cell Death Dis

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“…miRNA is a distinct class of small (approximately 22 nucleotides), single-stranded, and non-coding RNAs, and play critical functions in the regulation of cellular gene expression by binding to complementary sequences in the target mRNAs, leading to either translational repression or target degradation of the specific mRNAs ( Ha and Kim, 2014 ). Several studies indicate that miRNA carried by EVs plays a key role in the treatment of POI ( Xiao et al, 2016 ; Ding et al, 2020 ; Geng et al, 2022b ; Cai et al, 2022 ). In the following section, we explore the potential mechanisms and application value of different EVs ( Figure 1 ).…”

Section: The Pathogenesis Of Poi and The Application Of Stem Cell-der...mentioning

confidence: 99%

“…Subsequently, AFSCs-EVs are isolated and used in POI. Geng et al (2022b) indicate that CD44+/CD105+ human AFSC-EVs carrying miR-369-3p could specifically downregulate the expression of YAF2, inhibit the stability of PDCD5/p53, and reduce the apoptosis of OGCs, thereby exerting therapeutic effects on POI. In addition, compared with BMSCs, AFSCs secreted higher levels of EVs ( Tracy et al, 2019 ).…”

Section: Amniotic Fluid Stem Cell-derived Evs (Afscs-evs)mentioning

confidence: 99%

Stem cell-derived extracellular vesicles: A novel and potential remedy for primary ovarian insufficiency

Geng

Guo²,

Li³

et al. 2023

Front. Cell Dev. Biol.

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Primary ovarian insufficiency (POI) is an essential cause of young female fertility loss. At present, there are many treatments for primary ovarian insufficiency, but due to the complexity of the pathogenesis of primary ovarian insufficiency, the efficacy still could not be satisfactory. Stem cell transplantation is a feasible intervention protocol for primary ovarian insufficiency. However, its wide application in the clinic is limited by some defects such as tumorigenic and controversial ethical issues. Stem cell-derived extracellular vesicles (EVs) represent an important mode of intercellular communication attracting increasing interest. It is well documented that stem cell-derived extracellular vesicles for primary ovarian insufficiency with exciting therapeutic effects. Studies have found that stem cell-derived extracellular vesicles could improve ovarian reserve, increase the growth of follicles, reduce follicle atresia, and restore hormone levels of FSH and E2. Its mechanisms include inhibiting ovarian granulosa cells (GCs) apoptosis, reactive oxygen species, and inflammatory response and promoting granulosa cells proliferation and angiogenesis. Thus, stem cell-derived extracellular vesicles are a promising and potential method for primary ovarian insufficiency patients. However, stem cell-derived extracellular vesicles are still a long way from clinical translation. This review will provide an overview of the role and the mechanisms of stem cell-derived extracellular vesicles in primary ovarian insufficiency, and further elaborate on the current challenges. It may suggest new directions for future research.

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“…Obviously, the diverse spectrum of factors secreted by fetal stem cells (Table 1 ) already suggested that their restorative potential could be composed of many different mediators. Although the according research is still in its infancy, some signalling cascades including the nerve growth factor (NGF)/tropomyosin receptor kinase (TrkA) pathway [ 163 ], the phosphoinositide 3-kinase (PI3K) pathway [ 167 ], the transforming growth factor β1 (TGFβ1)/SMAD3 pathway [ 168 ], the bone morphogenetic protein (BMP)/SMAD pathway [ 169 ], epidermal growth factor (EGF)-mediated nuclear factor erythroid 2-related factor 2 (NRF2) /heme oxygenase-1 (HO-1) activation [ 170 ], the forkhead-box-protein O3 (FOXO3) pathway [ 167 ], the miR-369-3p/YY1-associated factor 2 (YAF2)/programmed cell death 5 (PDCD5)/p53 pathway [ 171 ], as well as the function of the hepatocyte growth factor (HGF) [ 172 ], epidermal growth factor (EGF) [ 172 ], or vascular endothelial growth factor (VEGF) [ 173 ], have already been demonstrated to be involved in the here discussed fetal stem cell-mediated curative processes (see also Table 3 ).…”

Section: Multipotent Fetal Stem Cells As New Therapeutic Tools For Re...mentioning

confidence: 99%

Multipotent fetal stem cells in reproductive biology research

et al. 2023

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Due to the limited accessibility of the in vivo situation, the scarcity of the human tissue, legal constraints, and ethical considerations, the underlying molecular mechanisms of disorders, such as preeclampsia, the pathological consequences of fetomaternal microchimerism, or infertility, are still not fully understood. And although substantial progress has already been made, the therapeutic strategies for reproductive system diseases are still facing limitations. In the recent years, it became more and more evident that stem cells are powerful tools for basic research in human reproduction and stem cell-based approaches moved into the center of endeavors to establish new clinical concepts. Multipotent fetal stem cells derived from the amniotic fluid, amniotic membrane, chorion leave, Wharton´s jelly, or placenta came to the fore because they are easy to acquire, are not associated with ethical concerns or covered by strict legal restrictions, and can be banked for autologous utilization later in life. Compared to adult stem cells, they exhibit a significantly higher differentiation potential and are much easier to propagate in vitro. Compared to pluripotent stem cells, they harbor less mutations, are not tumorigenic, and exhibit low immunogenicity. Studies on multipotent fetal stem cells can be invaluable to gain knowledge on the development of dysfunctional fetal cell types, to characterize the fetal stem cells migrating into the body of a pregnant woman in the context of fetomaternal microchimerism, and to obtain a more comprehensive picture of germ cell development in the course of in vitro differentiation experiments. The in vivo transplantation of fetal stem cells or their paracrine factors can mediate therapeutic effects in preeclampsia and can restore reproductive organ functions. Together with the use of fetal stem cell-derived gametes, such strategies could once help individuals, who do not develop functional gametes, to conceive genetically related children. Although there is still a long way to go, these developments regarding the usage of multipotent fetal stem cells in the clinic should continuously be accompanied by a wide and detailed ethical discussion.

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Human Amniotic Fluid Mesenchymal Stem Cell-Derived Exosomes Inhibit Apoptosis in Ovarian Granulosa Cell via miR-369-3p/YAF2/PDCD5/p53 Pathway

Cited by 29 publications

References 43 publications

The dual role of mesenchymal stem cells in apoptosis regulation

The dual role of mesenchymal stem cells in apoptosis regulation

Stem cell-derived extracellular vesicles: A novel and potential remedy for primary ovarian insufficiency

Multipotent fetal stem cells in reproductive biology research

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