Human amniotic epithelial cells induce apoptosis of cancer cells: a new anti-tumor therapeutic strategy

Niknejad, Hassan; Khayat-Khoei, Mahsa; Peirovi, Habibollah; Abolghasemi, Hassan

doi:10.1016/j.jcyt.2013.07.005

Cited by 79 publications

(68 citation statements)

References 19 publications

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“…Regarding the anticarcinogenic effect of hAM-derived epithelial cells (hAECs), Kang et al [10] reported that hAECs were able to inhibit in vitro and in vivo breast cancer cells. In addition, a significant increase in apoptosis rate were observed in cancer cells treated with hAECs conditioned medium [11].…”

Section: Introductionmentioning

confidence: 94%

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Mamede

Guerra

Laranjo

et al. 2016

Pathol. Oncol. Res.

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Section: Introductionmentioning

confidence: 94%

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Mamede

Guerra

Laranjo

et al. 2016

Pathol. Oncol. Res.

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“…One of the exciting new ideas that have been recently proposed in the field of cancer therapy using stem cells is the possible application of Amniotic Membrane (AM) in controlling cancer [5]. Even though there are published data on anti-cancer properties of the AM, the exact molecular mechanism of these effects are not yet clear and there are ongoing researches in the field that we aim to summarize and review here.…”

mentioning

confidence: 99%

“…This membrane is proven to have special characteristics such as antiangiogenic, immunoregulatory and pro-apoptotic [5,6] as well as antimicrobial, anti-fibrosis, and low immunogenicity properties [7]. The AM owes these properties to its unique histologic features of having two different types of stem cells: A single layer of Amniotic Epithelial Stem Cells (AESCs) on a thick basement membrane, and Amniotic Mesenchymal Stem Cells (AMSCs) in an avascular stroma.…”

mentioning

confidence: 99%

“…They suggested pro-apoptotic activities as well as anti-angiogenic and immunoregulatory capacities for the AM and argued that these properties can be applicable in cancer therapy. The hypothesised anti-cancer effects of the AM was further discussed and evaluated by Niknejad et al [5,9], Jiao et al [10] and Magatti et al [11].…”

mentioning

confidence: 99%

“…Providing a proper blood supply for tumor cells is a key element in tumor growth and metastasis [5] and the AM is believed to possess anti-angiogenesis properties [5,6,8,9,12]. There are reports of antiangiogenic factors such as collagen XVIII, thrombospondin-1, interleukin (IL)-1 receptor antagonist, IL-10 and tissue inhibitor of metalloprotease (TIMP-1, TIMP-2, TIMP-3, and TIMP-4) being mainly produced by the AM-derived Epithelial Stem Cells [12].…”

mentioning

confidence: 99%

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Role of Stem Cells in the Anti-tumor Effects of the Human Amniotic Membrane

Khayat-Khoei¹

2015

J Cytol Histol

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The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

Aziz

Fardyazar

Pashaiasl

2019

Molec Gen & Gen Med

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Purpose One of the most common malignancies peculiar to female health with few symptoms, low response to therapy, difficult diagnosis, frequent relapse, and high mortality, is ovarian cancer. Thus, our experiment, using Human amniotic fluid mesenchymal stem cells (hAFMSCs) as a therapeutic tool, aims to find an efficient treatment approach for patients suffering from SKOV3 ovarian cancer. Material & Methods In this study, we obtained 5 ml amniotic fluid from 16–20 week pregnant women who underwent amniocentesis for routine prenatal diagnosis by karyotyping in Al‐Zahra Hospital of Tabriz University of Medical Sciences, Iran. Using trans wells in 24 wells plate, hAFMSCs were isolated from all samples, co‐cultured with SKOV3 ovarian cancer cell line, and characterized via flow cytometry and RT‐PCR. Human skin fibroblast cells (HSFCs) were isolated and used as a negative control. SKOV3 and HSFCs' viability after 5 days was evaluated by MTT assay. Cell cycle and apoptotic genes were analyzed by real‐time PCR. Results We successfully isolated and characterized hAFMSCs through it positivity for CD44 and CD90 specific mesenchymal stem cell markers and negativity for CD31 and CD45. Oct4 and NANOG were evaluated by RT‐PCR as pluripotency markers, and visualized on 2% gel electrophoresis. We established hAFMS cell lines after 5 days of co‐culturing the SKOV3 cells, viability was decreased; however, HSFCs did not show toxicity by MTT assay. The genes indicated upregulation and high expression by a real‐time PCR. Conclusions Our findings showed that hAFMSCs have natural tumor tropism, and can release soluble factors in a cell culture, which cause an efficient anticancer effect. Thus, we can use hAFMSCs for complete anticancer therapy on SKOV3 cell line at cell culture condition and possibly in vivo in the near future.

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Human amniotic epithelial cells induce apoptosis of cancer cells: a new anti-tumor therapeutic strategy

Cited by 79 publications

References 19 publications

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Role of Stem Cells in the Anti-tumor Effects of the Human Amniotic Membrane

The human amniotic fluid mesenchymal stem cells therapy on, SKOV3, ovarian cancer cell line

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