2017
DOI: 10.1172/jci.insight.94375
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Human alternative Klotho mRNA is a nonsense-mediated mRNA decay target inefficiently spliced in renal disease

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Cited by 53 publications
(62 citation statements)
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“…There is good evidence that αKlotho mRNA may be transcribed but not translated into protein. Mencke et al provided convincing data that the so‐called “spliced Klotho mRNA” is actually destined for degradation by nonsense‐mediated mRNA decay and not translated into protein. RT‐PCR is very sensitive and could amplify non‐specific targets especially when a high cycle number is used.…”
Section: Discussionmentioning
confidence: 99%
“…There is good evidence that αKlotho mRNA may be transcribed but not translated into protein. Mencke et al provided convincing data that the so‐called “spliced Klotho mRNA” is actually destined for degradation by nonsense‐mediated mRNA decay and not translated into protein. RT‐PCR is very sensitive and could amplify non‐specific targets especially when a high cycle number is used.…”
Section: Discussionmentioning
confidence: 99%
“…In subsequent work, NMD targets are observed at greater levels in the monosome fraction than in the polysome fractions in yeast (Heyer & Moore, 2016). Additionally, predicted NMD-targeted transcripts were depleted from the polysome fraction, relative to the total cytoplasm or monosome fraction, in human cells (Sterne-Weiler et al, 2013; Floor & Doudna, 2016; Kim et al, 2017; Mencke et al, 2017). These findings are consistent with the evidence that many NMD targets are recognized in the pioneer round of translation (Ishigaki et al, 2001; Chiu et al, 2004).…”
Section: Introductionmentioning
confidence: 99%
“…8 Apart from the transmembrane form, Klotho has been detected in the urine, blood, and cerebrospinal fluid and may be generated mainly by proteolytic cleavage at the membrane by ADAM metallopeptidase domain proteases, ADAM10 and ADAM17 (soluble form), or through a splicing variant (secreted form), [9][10][11][12][13][14][15] the fate of which has been recently debated since this mRNA may be rapidly degraded. 16 Renal Klotho levels decline in experimental models of Chronic Kidney Disease (CKD). This likely contributes directly to hyperphosphatemia, increased vascular calcification, and cardiac hypertrophy, but potentially also has indirect effects via the parallel elevation of FGF-23.…”
Section: Introductionmentioning
confidence: 99%