Human adrenocortical carcinoma cell line (NCI-H295R): An in vitro screening model for the assessment of endocrine disruptors’ actions on steroidogenesis with an emphasis on cell ultrastructural features

Ďúranová, Hana; Fialková, Veronika; Válková, Veronika; Bilčíková, Jana; Olexíková, Lucia; Lukáč, Norbert; Massányi, Péter; Knazicka, Zuzana

doi:10.1016/j.acthis.2022.151912

Cited by 5 publications

(1 citation statement)

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“…Common screening methods: Effects on steroidogenesis are often analysed using the NCI-H295R cell line, which has emerged as a screening tool for adrenocortical physiology and potential endocrine disruption [94][95][96]. This is already implemented in the screening panel for the hazard identification of newly developed chemicals, proposed by the OECD and the US-EPA.…”

Section: Timementioning

confidence: 99%

Endocrine Disruptors: Focus on the Adrenal Cortex

Pötzl,

Kürzinger,

Stopper

et al. 2023

Horm Metab Res

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Endocrine-disrupting chemicals (EDCs) are exogenous substances known to interfere with endocrine homeostasis and promote adverse health outcomes. Their impact on the adrenal cortex, corticosteroids and their physiological role in the organism has not yet been sufficiently elucidated. In this review, we collect experimental and epidemiological evidence on adrenal disruption by relevant endocrine disruptors. In vitro data suggest significant alterations of gene expression, cell signalling, steroid production, steroid distribution, and action. Additionally, morphological studies revealed disturbances in tissue organization and development, local inflammation, and zone-specific hyperplasia. Finally, endocrine circuits, such as the hypothalamic-pituitary-adrenal axis, might be affected by EDCs. Many questions regarding the detection of steroidogenesis disruption and the effects of combined toxicity remain unanswered. Not only due to the diverse mode of action of adrenal steroids and their implication in many common diseases, there is no doubt that further research on endocrine disruption of the adrenocortical system is needed.

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Section: Timementioning

confidence: 99%