Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

Abstract

Angiogenesis is a complicated process in which perivascular cells play important roles. Multipotent mesenchymal stem/stromal cells (MSCs) from distinct tissues have been proved to be proangiogenic and share functional properties and gene expression profiles with perivascular cells. However, different tissues derived MSCs may exhibit different potential for clinical applications. Accordingly, comparative studies on different MSCs are essential. Here, we characterized MSCs from adipose (ADSCs), umbilical cord (UCMSCs), and endometrium (EMSCs) in terms of the surface antigen expression, differentiation ability, and the ability of angiogenesis promotion on endothelial colony-forming cells (ECFCs) both in vitro and in vivo. No significant differences in immunophenotype and differentiation were observed. In addition, three types of MSCs all located around tubular-like structures formed by ECFCs in coculture system on matrigel. But ECFCs seeded on ADSCs monolayer formed more organized capillary-like network than that on UCMSCs or EMSCs. When suspended with ECFCs in matrigel and implanted into nude mice, ADSCs promoted more functional vessel formation after 7 days. Moreover, in murine hindlimb ischemia model, cotransplantation of ECFCs with ADSCs was significantly superior to UCMSCs and EMSCs in promoting perfusion recovery and limb salvage. Furthermore, ADSC-conditioned medium (CM) contained more proangiogenic factors (such as vascular endothelial growth factor-A, platelet-derived growth factor BB, and basic fibroblast growth factor) and less inhibitory factor (such as thrombospondin-1), when compared with UCMSC-CM and EMSC-CM. And ADSC-CM more durably stabilized the vascular-like structures formed by ECFCs on matrigel and promoted ECFCs migration more efficiently. In summary, MSCs from adipose show significantly efficient promotion on angiogenesis both in vitro and in vivo than UCMSCs and EMSCs. Hence, ADSCs may be recommended as a more suitable source for treating hindlimb ischemia.

Mesenchymal Stem Cells Alleviate DHEA-Induced Polycystic Ovary Syndrome (PCOS) by Inhibiting Inflammation in Mice

“…The capacity of MSC for multilineage differentiation as well as immunomodulation has meant that MSCs are highly versatile for a wide range of therapeutic application. A number of animal model and translational studies have reported that MSC could home to sites of injury and/or inflammation and participate in tissue repair by cell replacement, secreting cytokines, and regulating immune response [22, 23, 34]. The use of MSCs for in immune/inflammation-related diseases appears to yield more efficacy than for cell replacement/tissue regeneration.…”

Therapeutic Potential of Endothelial Colony-Forming Cells in Ischemic Disease: Strategies to Improve their Regenerative Efficacy

“…An independent investigation showed that MSCs did not need to release paracrine signals to promote a microenvironment conducive for ECFC network formation [ 245 ]. Conversely, adipose-derived mesenchymal stem cells primed ECFCs to undergo bidimensional tube formation in vitro and assembly into patent vessels in vivo, thereby restoring local blood in a murine model of hindlimb ischemia, by releasing multiple growth factors, including VEGF-A, bFGF, and PDGF-BB [ 246 ]. Therefore, priming ECFCs with MSCs represents a promising strategy to improve the therapeutic efficacy of autologous ECFCs in ischemic disorders ( Table 7 ).…”

A novel method to improve the osteogenesis capacity of hUCMSCs with dual‐directional pre‐induction under screened co‐culture conditions

“…13,14 Considering that VEGF plays a vital role in facilitating angiogenesis as well as stimulating osteogenesis by regulating related growth factors, osteoblasts, BMSCs, hUCMSCs or human adipose mesenchymal stem cells (hADMSCs) derived VEGF is much emphasized. 8,[15][16][17][18] An optimal concentration of VEGF is required for angiogenesis and osteogenesis coupling for intramembranous ossification on account that a higher or lower concentration of VEGF could result in compromised bone formation. 17 In light of these phenomena, a co-culture system of osteogenic cells (stem cells, osteoblasts, etc) and angiogenic cells (endothelial progenitor cells, endothelial cells, etc) has been raised, which has been validated there is a synergistic effect in promoting osteogenesis and angiogenesis, favourable for the survival and prognosis of osteoblasts in the scaffolds, thus ensuring a successful ossification of tissue-engineered bone.…”