Human adipose-derived stem cells isolated from young and elderly women: their differentiation potential and scaffold interaction during in vitro osteoblastic differentiation

Girolamo, Laura; Lopa, Silvia; Arrigoni, E.; Sartori, Matteo; Preis, Franz Wilhelm Baruffaldi; Brini, A.T.

doi:10.1080/14653240903079393

Cited by 28 publications

(52 citation statements)

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“…Alternative sources that both abate surrounding ethical issues and previously have shown potential for osteoblast generation include umbilical cord stem cells, mesenchymal stem cells, and adiposederived stem cells. [23][24][25] This study employed the use of mouse ES cells as a robust model of early differentiation. Although findings shown here may not necessarily be transferred directly to a human ES cell model, the similarities that exist between osteogenic differentiation of human and mouse ES cells would suggest it is likely.…”

Section: Introductionmentioning

confidence: 99%

Engineering Embryonic Stem-Cell Aggregation Allows an Enhanced Osteogenic Differentiation In Vitro

Gothard

Roberts

Shakesheff

et al. 2010

Tissue Engineering Part C: Methods

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Pluripotent embryonic stem (ES) cells hold great promise for the field of tissue engineering, with numerous studies investigating differentiation into various cell types including cardiomyocytes, chondrocytes, and osteoblasts. Previous studies have detailed osteogenic differentiation via dissociated embryoid body (EB) culture in osteoinductive media comprising of ascorbic acid, beta-glycerophosphate, and dexamethasone. It is hoped that these osteogenic cultures will have clinical application in bone tissue repair and regeneration and pharmacological testing. However, differentiation remains highly inefficient and generates heterogeneous populations. We have previously reported an engineered three-dimensional culture system for controlled ES cell-ES cell interaction via the avidin-biotin binding complex. Here we investigate the effect of such engineering on ES cell differentiation. Engineered EBs exhibit enhanced osteogenic differentiation assessed by cadherin-11, Runx2, and osteopontin expression, alkaline phosphatase activity, and bone nodule formation. Results show that cultures produced from intact EBs aggregated for 3 days generated the greatest levels of osteogenic differentiation when cultured in osteoinductive media. However, when cultured in control media, only engineered samples appeared to exhibit bone nodule formation. In addition, polymerase chain reaction analysis revealed a decrease in endoderm and ectoderm expression within engineered samples. This suggests that engineered ES cell aggregation has increased mesoderm homogeneity, contributing to enhanced osteogenic differentiation.

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Section: Introductionmentioning

confidence: 99%

Engineering Embryonic Stem-Cell Aggregation Allows an Enhanced Osteogenic Differentiation In Vitro

Gothard

Roberts

Shakesheff

et al. 2010

Tissue Engineering Part C: Methods

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show abstract

“…Data in the literature show that 30-year-old donors respond more positively to an EGF stimulus than do older donors above the age of 50 (Zhu and Joyce 2004). However, older-donor-derived cells show higher adipocytic differentiation (Girolamo et al 2009). Furthermore, differences have been observed in the adipogenic, chondrogenic and osteogenic differentiation capacity of murine MSCs harvested from donor animals of different ages and in the number of cell passages (Kretlow et al 2008).…”

Section: Discussionmentioning

confidence: 95%

“…In the body, stem cells of many tissues reside in specialised structures termed niches and their potential is age-dependent (Hermann et al 2010;Fuchs et al 2004;Girolamo et al 2009). Transferring the stem cells outside their niche might change cellular responses and stem cells might respond differently from non-stem cells.…”

Section: Introductionmentioning

confidence: 97%

“…Indeed, significant donor to donor variability in the expression of neuralrelated markers by bone-marrow-derived mesenchymal stromal cells has been found (Montzka et al 2009). Human adipose-derived stem cells isolated from young and elderly women differ in their osteoblast differentiation potential and the yield of the cells from older donors is significantly greater than that from younger donors (Girolamo et al 2009). Muscle-derived stem cells (MDSCs) have been shown to exhibit heterogeneity in marker profiles and vary in their regeneration ability.…”

Section: Introductionmentioning

confidence: 98%

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Culture-expanded human dermal stem cells exhibit donor to donor differences in cAMP generation

et al. 2011

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Stem cell techniques have facilitated a number of potential uses for such cells in cell therapy and drug development. Studies of the cAMP/protein kinase A (PKA) pathway are widely employed to investigate the effects of a large variety of substances. We assayed the cAMP pathway in human skin-derived mesenchymal stem cells (S-MSC) to evaluate donor to donor variations in response to pharmacological manipulations in vitro. Immunophenotyping of S-MSC revealed that, in general, 95% of S-MSCs were positive for CD90, CD73 and CD105 and negative for the expression of haemopoetic markers CD14, CD45 and human leukocyte antigen-DR (HLA-DR). Nevertheless, fluctuations occurred in basal cAMP levels from 5 pmol/mg to 18 pmol/mg. Total cAMP response element binding protein (CREB) concentrations ranged from 0.8 ng/ml to 1 ng/ml, whereas the proportions of phospho-CREB versus total CREB differed between the cell lines. Basic fibroblast growth factor (FGF-2) and epidermal growth factor (EGF) stimulated cAMP generation, whereas leukaemia inhibiting factor reduced some of their effects. Forskolin (0.05 and 1 mM) acted in synergy with FGF-2 and EGF; however, it caused pronounced donor to donor differences in the increase of cAMP and phospho-CREB levels. Additionally, dibutyryl-cAMP caused significant donor to donor variations in cell proliferation, possibly indicating a change of cell differentiation status. We speculate that similar donor diversity might be observed after cell stimulation with various G(s)-protein-coupled receptor ligands. Heterogeneity of donor cell responses to stimulation of the cAMP pathway indicates the need for wide safety margins for S-MSC use in drug screening; nevertheless, knowledge of this heterogeneity might be useful for the design of donor-specific cell therapy.

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“…6,[11][12][13][14] In addition to the in vitro manipulation procedures, the original properties of the donor, such as the inherent and acquired factors (eg, age, sex, tissue, and underlying physiology or pathology), are contributed to the variance of mesenchymal stem cell (MSC) proliferation capacity and differentiation potency. [15][16][17][18] Therefore, it is important to establish assessing parameters for evaluating the long-term proliferation potency of ADSCs, especially in the case of quality control of large-scale cell manufacturing procedures. In the present study, we comparatively analyzed lipoaspirate specimens obtained using suction-assisted syringe techniques, third-generation ultrasound-assisted liposuction (vibration amplification of sound energy at resonance, abbreviated as VASER), and water-jet-assisted liposuction, in the aspects of lipoaspirate tissue viability, SVF yield, and ADSC characteristics.…”

mentioning

confidence: 99%

Selective Criteria and Markers in Adipose-Derived Stromal Cells Collection Quality and Expansion Potency

Chen

Tseng²,

Huang

et al. 2016

Annals of Plastic Surgery

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Human adipose-derived stem cells isolated from young and elderly women: their differentiation potential and scaffold interaction during in vitro osteoblastic differentiation

Cited by 28 publications

References 0 publications

Engineering Embryonic Stem-Cell Aggregation Allows an Enhanced Osteogenic Differentiation In Vitro

Engineering Embryonic Stem-Cell Aggregation Allows an Enhanced Osteogenic Differentiation In Vitro

Culture-expanded human dermal stem cells exhibit donor to donor differences in cAMP generation

Selective Criteria and Markers in Adipose-Derived Stromal Cells Collection Quality and Expansion Potency

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