Human Adipose Derived Stem Cells and Osteoblasts Interaction with Fe–Cr–Nb–B Magnetic Nanoparticles

Lăbuşcă, Luminiţa; Herea, Dumitru-Daniel; Radu, Ecaterina; Danceanu, Camelia; Chiriac, H.; Lupu, Nicoleta

doi:10.1166/jnn.2018.15330

Cited by 7 publications

(13 citation statements)

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“…Follow-up of the fate of administered stem cells using combined imaging methods has been proposed as a method to discriminate tumorigenic transformation. In the future, this technology can be used to monitor liver cancer after SCT[ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of autologous stem cell transplantation for decompensated liver cirrhosis: A retrospective cohort study

Wang¹,

Li²,

Gao³

et al. 2018

WJSC

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AIMTo evaluate the long-term efficacy and safety of autologous stem cell transplantation (SCT) for decompensated liver cirrhosis.METHODSConsecutive patients with decompensated liver cirrhosis were included and assigned into the SCT group and non-transplantation (non-SCT) group according to whether they received SCT treatment. Patients were followed up for ten years. The long-term survival rate and incidence of hepatocellular carcinoma (HCC) were compared between groups.RESULTSA total of 159 patients were enrolled, including 27 cases in the SCT group and 132 cases in the non-SCT group. The baseline characteristics were significantly different between the two groups. Propensity score matching (PSM) was used to match SCT and non-SCT patients. After PSM, 92 subjects were enrolled in the final analysis, including 23 cases in the SCT group and 69 cases in the non-SCT group. The overall mortality was 73.9% and 55.1%, and the median survival period was 48 and 64 mo, respectively. However, no significant difference was found in the long-term survival rate between the two groups (P > 0.05). In addition, the incidence of HCC was higher in the SCT group than in the non-SCT group (47.8% vs 21.7%, P < 0.05). After adjusting for other covariates, SCT (OR = 3.065, 95%CI: 1.378-6.814) and age (OR = 1.061, 95%CI: 1.021-1.102) were independently correlated with the development of HCC in this decompensated liver cirrhosis cohort.CONCLUSIONAutologous SCT may fail to improve the long-term efficacy and increase the incidence of HCC for decompensated liver cirrhosis. Close monitoring of HCC is strongly recommended in patients undergoing autologous SCT.

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Section: Discussionmentioning

confidence: 99%

Efficacy and safety of autologous stem cell transplantation for decompensated liver cirrhosis: A retrospective cohort study

Wang¹,

Li²,

Gao³

et al. 2018

WJSC

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“…Recently, stem cells have been proposed as suitable carriers for tumor-targeting therapeutics 25 . In our previous work 26 , 27 , we have investigated the interaction of Fe-Cr-Nb-B MPs with human adipose-derived mesenchymal stem cells (MSCs) and human bone marrow-derived MSCs. It was observed that Fe-Cr-Nb-B MPs have been internalized by both types of cells and did not induce in vitro cytotoxicity, indicating a good biocompatibility.…”

Section: Magnetic Particles and Magnetic Ferrofluidmentioning

confidence: 99%

Fe-Cr-Nb-B ferromagnetic particles with shape anisotropy for cancer cell destruction by magneto-mechanical actuation

Chiriac

Radu

Ţibu

et al. 2018

Sci Rep

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We introduce a new type of magnetic particles (MPs) prepared by wet milling of superferromagnetic Fe-Cr-Nb-B precursor glassy ribbons for cancer treatment by magneto-mechanical actuation in low magnetic fields (1 ÷ 20 Oe). The rectangular shapes of MPs and the superferromagnetism of the glassy alloys of which are made the MPs induce important magnetic shape anisotropies which, in association with a large saturation magnetization, generate an improved torque in a rotating magnetic field, producing important damages on the cellular viability of MG-63 human osteosarcoma (HOS) cells. The specific parameters such as MPs concentration, frequency and intensity of the applied magnetic field, or the time of exposure have a strong influence on the cancer cells viability. The specific behavior of the Fe-Cr-Nb-B MPs offers them destructive effect even in low magnetic fields such as 10 Oe, and this characteristic allows the use of coils systems which provide large experimental spaces. The novel MPs are used for the magneto-mechanical actuation alone or in association with hyperthermia, but also can be transported to the tumor sites by means of stem cells carriers.

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“…Human primary ADSC were obtained from healthy donors undergoing liposuction procedures for cosmetic reasons after institutional board ethical approval and written informed patient consent. Lipoaspirate was processed within 24 hr after surgical procedure as previously described 13 . Briefly, lipoaspirate was washed with PBS, enzymatically digested with collagenase type I (0.01 mg/ml) for 2 hr at 37.5°C, and centrifuged at 300 g for 5 min at room temperature (RT).…”

Section: Methodsmentioning

confidence: 99%

“…Excellent biocompatibility, improved proliferation as well as preserved or even increased differentiation potential has been reported for MSC of human origin loaded with MNP of various compositions and coatings 13,14 . Magnetic actuation has been used in testing various remote controlled systems for therapeutic purposes 15 .…”

Section: Introductionmentioning

confidence: 99%

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Magnetic nanoparticle loaded human adipose derived mesenchymal cells spheroids in levitated culture

et al. 2020

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Magnetic nanoparticles (MNP) are intensely scrutinized for biomedical applications due to their excellent biocompatibility and adjustable magnetic field (MF) responsiveness. Three‐dimensional spheroid culture of ADSC improves stem cell proliferation and differentiation, increasing their potential for clinical applications. In this study we aimed to detect if MF levitated culture of ADSC loaded with proprietary MNP maintain the properties of ADSC and improve their performances. Levitated ADSC‐MNP formed aggregates with increased volume and reduced number compared to nonlevitated ones. ADSC‐MNP from levitated spheroid displayed higher viability, proliferation and mobility compared to nonlevitated and 2D culture. Levitated and nonlevitated ADSC‐MNP spheroids underwent three lineage differentiation, demonstrating preserved ADSC stemness. Quantitative osteogenesis showed similar values in MNP‐loaded levitated and nonlevitated spheroids. Significant increases in adipogenic conversion was observed for all 3D formulation. Chondrogenic conversion in levitated and nonlevitated spheroids produced comparable ratio glucosaminoglycan (GAG)/DNA. Increased chondrogenesis could be observed for ADSC‐MNP in both levitated and nonlevitated condition. Taken together, ADSC‐MNP levitated spheroids retain stemness and display superior cell viability and migratory capabilities. Furthermore, the method consistently increases spheroid maneuverability, potentially facilitating large scale manufacturing and automation. Levitated spheroid culture of ADSC‐MNP can be further tested for various application in regenerative medicine and organ modeling.

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Human Adipose Derived Stem Cells and Osteoblasts Interaction with Fe–Cr–Nb–B Magnetic Nanoparticles

Cited by 7 publications

References 0 publications

Efficacy and safety of autologous stem cell transplantation for decompensated liver cirrhosis: A retrospective cohort study

Efficacy and safety of autologous stem cell transplantation for decompensated liver cirrhosis: A retrospective cohort study

Fe-Cr-Nb-B ferromagnetic particles with shape anisotropy for cancer cell destruction by magneto-mechanical actuation

Magnetic nanoparticle loaded human adipose derived mesenchymal cells spheroids in levitated culture

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