Human adenoviruses in respiratory infections: Sequencing of the hexon hypervariable region reveals high sequence variability

Biere, Barbara; Schweiger, Brunhilde

doi:10.1016/j.jcv.2010.01.005

Cited by 29 publications

(18 citation statements)

References 24 publications

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“…Ad1p differed from the reference strain used in the phylogenetic tree by a single nucleotide. It was present in 2005 and 2006 and has also been reported in many Ad1 isolates from patients in Yamagata, Japan from 1998 to 2007 (Mizuta et al, 2009) and by Biere and Schweiger (2010) as Ad1π in Germany from 2001 to 2007. Ad1v2 was found in two patients between July 2005 and May 2006.…”

Section: Resultsmentioning

confidence: 55%

“…Fujimoto et al (2008); 5. Biere and Schweiger (2010); 6. reported from Taiwan; 7. Takeuchi et al (1999); 8.…”

Section: Resultsmentioning

confidence: 99%

“…It was reported also as a US Navy field strain isolated in 2003 from a patient with febrile respiratory illness (Lin et al, 2006). The variant was represented in several clinical samples from German patients with respiratory infections between 2001 and 2007 and designated genetic lineage Ad3γ (Biere and Schweiger, 2010) and in several isolates from throat swabs from patients in Taiwan in 2007. Ad11p was detected in 2004 in a urine sample also from a single patient.…”

Section: Resultsmentioning

confidence: 99%

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Sequence typing of adenovirus from samples from hematological stem cell transplant recipients

Qurashi

Guiver²,

Cooper

2011

Journal of Medical Virology

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Adenovirus infections are usually mild or even asymptomatic, but infections with the virus are being recognized increasingly as a major cause of mortality and morbidity in the immunocompromised, particularly hematological stem cell transplant patients where infections can be life threatening and mortality may reach 60%. Typing by sequencing the HVR7 region of the hexon was established and validated using 60 isolates of different serotypes from the six of the seven species which had been typed previously by serum neutralization. Analysis of nucleotide sequences was used to type 227 samples from 41 hematological stem cell transplant recipients. Types from six species were detected but species C types were detected in 51.4% and species A in 34.3% of patients. Seven patients were infected with different adenovirus types sequentially and a further six patients had evidence of simultaneous multiple infections. Many of the sequences had several differences from the prototype strains which will allow tracing of outbreaks and provide evidence for cross-infection in a hospital setting. In this study, the phylogenetic analysis of adenovirus sequences from hematological stem cell transplant patients' samples showed evidence of two possible cross-infection incidents involving three and five patients, respectively.

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Section: Resultsmentioning

confidence: 55%

“…Fujimoto et al (2008); 5. Biere and Schweiger (2010); 6. reported from Taiwan; 7. Takeuchi et al (1999); 8.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Sequence typing of adenovirus from samples from hematological stem cell transplant recipients

Qurashi

Guiver²,

Cooper

2011

Journal of Medical Virology

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“…Several molecular epidemiology studies found that the HAdV-3 hexon sequences have a high variability mainly in the HVRs, including HVR1, HVR3, HVR6, and HVR7, and that they have formed multiple genetic lineages (Mahadevan et al, 2010;Choi et al, 2006;Fujimoto et al, 2008;Mizuta et al, 2009;Biere and Schweiger, 2010). Our results using the globally known HAdV-3 hexon sequences available in GenBank (Fig.…”

Section: Antiserumsupporting

confidence: 49%

Mapping the epitope of neutralizing monoclonal antibodies against human adenovirus type 3

Tian

Liu

et al. 2015

Virus Research

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“…The dominant species in circulation appears to be HAdV-C, though HAdV-B and HAdV-E have also been identified in patients with ILI. The circulation of HAdV-3, HAdV-5 and HAdV-7 is of note as these types are recognized causes of more severe acute respiratory infection [50]. Scientists and medical personnel concerned with the etiology of acute respiratory infections in Egypt should consider HAdV as a potential cause, given this evidence of infection among patients with ILI.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010

et al. 2014

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BackgroundHuman adenoviruses of species B, C, and E (HAdV-B, –C, -E) are frequent causative agents of acute respiratory infections worldwide. As part of a surveillance program aimed at identifying the etiology of influenza-like illness (ILI) in Egypt, we characterized 105 adenovirus isolates from clinical samples collected between 2003 and 2010.MethodsIdentification of the isolates as HAdV was accomplished by an immunofluorescence assay (IFA) and confirmed by a set of species and type specific polymerase chain reactions (PCR).ResultsOf the 105 isolates, 42% were identified as belonging to HAdV-B, 60% as HAdV–C, and 1% as HAdV-E. We identified a total of six co-infections by PCR, of which five were HAdV-B/HAdV-C co-infections, and one was a co-infection of two HAdV-C types: HAdV-5/HAdV-6. Molecular typing by PCR enabled the identification of eight genotypes of human adenoviruses; HAdV-3 (n = 22), HAdV-7 (n = 14), HAdV-11 (n = 8), HAdV-1 (n = 22), HAdV-2 (20), HAdV-5 (n = 15), HAdV-6 (n = 3) and HAdV-4 (n = 1). The most abundant species in the characterized collection of isolates was HAdV-C, which is concordant with existing data for worldwide epidemiology of HAdV respiratory infections.ConclusionsWe identified three species, HAdV-B, -C and -E, among patients with ILI over the course of 7 years in Egypt, with at least eight diverse types circulating.

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Human adenoviruses in respiratory infections: Sequencing of the hexon hypervariable region reveals high sequence variability

Cited by 29 publications

References 24 publications

Sequence typing of adenovirus from samples from hematological stem cell transplant recipients

Sequence typing of adenovirus from samples from hematological stem cell transplant recipients

Mapping the epitope of neutralizing monoclonal antibodies against human adenovirus type 3

Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010

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