Human adenovirus in municipal solid waste leachate and quantitative risk assessment of gastrointestinal illness to waste collectors

Lanzarini, Natália Maria; Federigi, Ileana; Mata, Rafaela Marinho; Borges, Maria Denise Neves; Saggioro, Enrico Mendes; Cioni, Lorenzo; Verani, Marco; Carducci, Annalaura; Moreira, Josino Costa; Mannarino, Camille Ferreira; Miagostovich, Marize Pereira

doi:10.1016/j.wasman.2021.12.005

Cited by 11 publications

(4 citation statements)

References 62 publications

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“…More studies are needed to confirm this hypothesis. The physicochemical characterization of the fresh truck leachate from this WTS was previously analyzed in 2019, showing an acidic pH, high apparent color, turbidity, total solids (dissolved, suspended, fixed, and volatile), total organic carbon, and chemical oxygen demand (Lanzarini et al 2022). The influence of household collection time on MSWL-BE could be related to the characteristics of SARS-CoV-2, as enveloped viruses are less persistent in the environment when compared with nonenveloped viruses (Wurtzer et al 2021).…”

Section: Resultsmentioning

confidence: 99%

“…This non-difference is related to the fact that SARS-CoV-2 genomes could be present in different forms: most parts as a ribonucleoprotein complex and as free/unprotected viral RNA, and less than 10% as protected within infectious or non-infectious particles, reinforcing non-infectivity (Wurtzer et al 2021). Previous studies have already detected infectious HAdV by capsid integrity assay and cell culture infectivity and estimated the quantitative microbial risk assessment for gastrointestinal illness disease (Lanzarini et al 2022).…”

Section: Resultsmentioning

confidence: 99%

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SARS-CoV-2 surveillance-based on municipal solid waste leachate in Brazil

Lanzarini

Mannarino

Ribeiro

et al. 2023

Environ Sci Pollut Res

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Municipal solid waste leachate-based epidemiology is an alternative viral tracking tool that applies fresh truck leachate as an early warning of public health emergencies. This study aimed to investigate the potential of SARS-CoV-2 surveillance based on solid waste fresh truck leachate. Twenty truck leachate samples were ultracentrifugated, nucleic acid extracted, and real-time RT-qPCR SARS-CoV-2 N1/N2 applied. Viral isolation, variant of concern (N1/N2) inference, and whole genome sequencing were also performed. SARS-CoV-2 was detected on 40% (8/20) of samples, with a concentration from 2.89 to 6.96 RNA Log 10 100 mL −1 . The attempt to isolate SARS-CoV-2 and recover the whole genome was not successful; however, positive samples were characterized as possible pre-variant of concern (pre-VOC), VOC Alpha (B.1.1.7) and variant of interest Zeta (P.2). This approach revealed an alternative tool to infer SARS-CoV-2 in the environment and may help the management of local surveillance, health, and social policies. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

SARS-CoV-2 surveillance-based on municipal solid waste leachate in Brazil

Lanzarini

Mannarino

Ribeiro

et al. 2023

Environ Sci Pollut Res

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“…The dose–response model for AdV risk assessment combined with the exponential model of Kongprajug et al [ 31 ] and Lanzarini et al [ 32 ].

…”

Section: Methodsmentioning

confidence: 99%

Quantitative Risk Assessment of Five Foodborne Viruses in Shellfish Based on Multiplex qPCR

Yu,

Xu,

Chen

et al. 2023

Foods

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Foodborne diseases are currently the most critical food safety issue in the world. There are not many hazard identification and exposure assessments for foodborne viruses (Norovirus GI, GII, Hepatitis A Virus, Rotavirus, Adenovirus) in shellfish. Multiplex qPCR for the simultaneous detection of five foodborne viruses was established and used to assess infection risk based on a 1-year pathogenesis study. The sensitivity, specificity and reproducibility of the multiplex qPCR method are consistent with that of conventional qPCR, which saves more time and effort. Overall, 37.86% of shellfish samples had one or more foodborne viruses. Risk assessment formulae and matrices were used to develop risk assessments for different age groups, different seasons and different shellfish. The annual probability of contracting a foodborne virus infection from shellfish is greater than 1.6 × 10−1 for all populations, and even for infants aged 0–4 years, it is greater than 1.5 × 10−2, which is much higher than the risk thresholds recommended by WHO (10−6) and the US EPA (10−4). High risk (level IV) is associated with springtime, and medium risk (level III) is associated with Mussel consumption. This study provides a basis for the risk of foodborne viral infections in people of different ages, in different seasons, and by consuming different shellfish.

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“…Nevertheless, the lack of epidemiological studies hampers the definition of limits for an acceptable risk. On the other hand, the study of microbial composition of bioaerosol with cultural or noncultural methods can show the presence and concentration of pathogens, allowing a more specific risk assessment (5,6). Nevertheless, the proposed methods are highly variable and often lacking validation and standardization for risk assessment purposes.…”

Section: Main Findings and Research Neededmentioning

confidence: 99%