Human Acellular Dermal Matrix in Reconstructive Surgery—A Review

Gierek, Marcin; Łabuś, Wojciech; Kitala, Diana; Lorek, Andrzej; Ochała-Gierek, Gabriela; Zagórska, Karolina Mikuś; Waniczek, Dariusz; Szyluk, Karol; Niemiec, Paweł

doi:10.3390/biomedicines10112870

Cited by 33 publications

(36 citation statements)

References 47 publications

(132 reference statements)

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“…In studies on reconstructive surgery, various biological materials including skin, acellular dermal matrix (biological collagen matrix), allograft, iliac or fibular graft, and devitalized bone graft have been reported [17,18]. Although allograft is one of the useful biological materials, there is a vital deficit of donors and the reserves of allografts are not sufficient.…”

Section: Discussionmentioning

confidence: 99%

Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities

Miwa

Yamamoto

Hayashi

et al. 2023

Cancers

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Tumor-bearing frozen autografts have been widely used for reconstruction of bone defects caused by tumor resection. However, some patients undergo removal of the grafted bone due to surgical site infection, tumor recurrence, or fractures of the grafted bone. In this retrospective cohort study, predictive factors for graft survival were investigated in 123 patients who underwent reconstructions using a tumor-bearing frozen autograft after bone tumor resection of the extremities. To determine the independent predictors of graft survival, the association between various parameters and graft survival was investigated. The graft survival rates were 83.2% at 5 years and 70.2% at 10 years. Among the 123 frozen autografts, 25 (20.3%) were removed because of complications. In univariate analyses, male sex, BMI of ≥23.6, tibia, and chemotherapy were significantly associated with poor graft survival, whereas the pedicle/hemicortical freezing procedure was significantly associated with better graft survival. Multivariate analysis using the Cox proportional hazards regression model revealed that BMI of ≥23.6 (HR, 3.4; p = 0.005), tibia (HR, 2.3; p = 0.047), and freezing procedure (HR, 0.3; p = 0.016) were independently associated with graft survival. Based on the results, pedicle or hemicortical freezing techniques are recommended in cases where these techniques can be applied.

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Section: Discussionmentioning

confidence: 99%

Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities

Miwa

Yamamoto

Hayashi

et al. 2023

Cancers

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“…[1] While other approaches involving skin substitutes or growth factors exist, their regenerative capacity is limited in cases of extensive damage, often necessitating additional surgeries, such as skin grafting. [2][3][4] Some products cultivated from fibroblasts are promising but face challenges when applied to extensive injuries. [5,6] Without proper support from the extracellular matrix and scaffold during transplantation, these materials can trigger inflammation, leading to a sharp decline in engraftment rates and raising doubts about their effectiveness.…”

Section: Introductionmentioning

confidence: 99%

The transdifferentiation of human dedifferentiated fat cells into fibroblasts: An in vitro experimental pilot study

Lim,

Jeon,

Kim

2024

Medicine

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Background: Skin grafting is a common method of treating damaged skin; however, surgical complications may arise in patients with poor health. Currently, no effective conservative treatment is available for extensive skin loss. Mature adipocytes, which constitute a substantial portion of adipose tissue, have recently emerged as a potential source of stemness. When de-lipidated, these cells exhibit fibroblast-like characteristics and the ability to redifferentiate, offering homogeneity and research utility as “dedifferentiated fat cells.” Methods and results: We conducted an in vitro study to induce fibroblast-like traits in the adipose tissue by transdifferentiating mature adipocytes for skin regeneration. Human subcutaneous fat tissues were isolated and purified from mature adipocytes that underwent a transformation process over 14 days of cultivation. Microscopic analysis revealed lipid degradation over time, ultimately transforming cells into fibroblast-like forms. Flow cytometry was used to verify their characteristics, highlighting markers such as CD90 and CD105 (mesenchymal stem cell markers) and CD56 and CD106 (for detecting fibroblast characteristics). Administering dedifferentiated fat cells with transforming growth factor-β at the identified optimal differentiation concentration of 5 ng/mL for a span of 14 days led to heightened expression of alpha smooth muscle actin and fibronectin, as evidenced by RNA and protein analysis. Meanwhile, functional validation through cell sorting demonstrated limited fibroblast marker expression in both treated and untreated cells after transdifferentiation by transforming growth factor-β. Conclusion: Although challenges remain in achieving more effective transformation and definitive fibroblast differentiation, our trial could pave the way for a novel skin regeneration treatment strategy.

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“…Collagen, an abundant structural protein nestled within the intricate dermal matrix, [8][9][10][11][12] stands as a pivotal guardian of the skin's foundational integrity. Although collagen boasts commendable biocompatibility and biological activity, it is vulnerable to enzymatic degradation in the body and has limited stability.…”

Section: Introductionmentioning

confidence: 99%

A highly biocompatible CE-crosslinked collagen implant with exceptional anti-calcification and collagen regeneration capabilities for aging skin rejuvenation

Wang,

Yan,

Yao

et al. 2024

J. Mater. Chem. B

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Human Acellular Dermal Matrix in Reconstructive Surgery—A Review

Cited by 33 publications

References 47 publications

Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities

Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities

The transdifferentiation of human dedifferentiated fat cells into fibroblasts: An in vitro experimental pilot study

A highly biocompatible CE-crosslinked collagen implant with exceptional anti-calcification and collagen regeneration capabilities for aging skin rejuvenation

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