hucMSCs transplantation promotes locomotor function recovery, reduces apoptosis and inhibits demyelination after SCI in rats

Liao, Ziling; Yang, Xuebo; Wang, Wei; Deng, Weiyue; Song, Aishi; Ni, Bin; Zhao, Huifang; Zhang, Shusheng; Li, Zhiyuan

doi:10.1016/j.npep.2021.102125

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…HUCMSCs were obtained through the enzymatic digestion of UC Wharton's jelly using collagenase 1.67% (Sigma, USA) at 37°C. Then HUCMSCs were cultured in cell culture plates using α-MEM(Gibco, USA) supplemented with 4 mM GlutaMax (Gibco, USA) and 10% inactivated fetal bovine serum (Cyagen, USA) and 1% streptomycin/penicillin in 37°C incubator with 5% CO2 [22].…”

Section: Isolation and Characterization Of Hucmscsmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

Luo

Zhang

et al. 2021

Preprint

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Background: Cerebral palsy (CP) is a brain injury disease, which is a global public health issue with an estimated prevalence of 2‰—4‰ and imposes a substantial health burden on many countries. At present, there is no ideal treatment available and most of them will still suffer adverse outcomes. Human umbilical cord mesenchymal stem cells(HUCMSCs) application in many fields of medicine, which can promote nervous system regeneration and inhibit neuroinflammation. The regeneration of central nervous system(CNS) is related to the nervous regeneration inhibitors. NogoA/NgR/Rho pathway is very important to the nerve growth, CP injury is inevitably accompanied by the regeneration and repair of neurons and axons. so we hypothesized that NogoA/NgR/Rho pathway is involved in when using the HUCMSCs to treatment cerebral palsy.Purpose: In this study, we might clarify the NogoA/NgR/Rho pathway functional role in mediating HUCMSCs to improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced CP rat model.Methods: The CP rat model was established by ligating the left common carotid artery and anoxia for 2.5 h, and HUCMSCs were intravenous injected to the modeled rats. The neurobehavioral situation and brain pathological injury in CP rats were determined via a series of assays. The mRNA and protein expression of NogoA、NgR、RhoA、Rac-1、Cdc42 in brain tissue of rats in each group was detected by RT-qPCR and western blot analysis. Results: The CP rats exhibited obvious motor function abnormalities, pathological damage and a lot of brain nerve cell apoptosis. Compared with CP+PBS group and CP group rats, HUCMSCs transplantation can significantly improve the neurobehavioral situation, attenuated brain pathological injury, inhibit apoptosis of brain nerve cells and the activation of astrocytes in CP rats. The expression of NogoA、NgR、RhoA relative mRNA and protein in brain tissues of rats in the CP+PBS group and CP group rats were significantly lower than those of in the sham+PBS and CP+HUCMSCs group. The expression of Rac-1、Cdc42 relative mRNA and protein in brain tissues of rats in the sham and CP+HUCMSCs group was significantly higher than those of in CP+PBS group and CP group rats. Conclusion: This study confirmed that HUCMSCs can efficiently improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced cerebral palsy rat model via down-regulating the NogoA/NgR/Rho pathway.

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Section: Isolation and Characterization Of Hucmscsmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

Luo

Zhang

et al. 2021

Preprint

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“…Human umbilical cord MSC (HUCMSC) are easy to isolate, culture, and amplify, with no restrictions on moral and ethical issues ( 13 ). HUCMSC transplantation can ameliorate motor function, tissue repairing and reducing apoptosis in spinal cord injury ( 14 ). It is also showed that HUCMSC transplantation may be a novel therapeutic approach for treating acute radiation injury ( 15 ), traumatic brain injury ( 16 ), acute kidney injury ( 17 ), radial nerve injury ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

RNA-Sequencing approach for exploring the therapeutic effect of umbilical cord mesenchymal stem/stromal cells on lipopolysaccharide-induced acute lung injury

Cui

Zhang²,

Pan³

et al. 2022

Front. Immunol.

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Acute lung injury (ALI) is significantly associated with morbidity and mortality in patients with critical diseases. In recent years, studies have identified that mesenchymal stem/stromal cells (MSCs) ameliorate ALI and pulmonary fibrosis. However, the mechanism underlying this outcome in ALI has not yet been investigated. In this study, RNA sequencing technology was used to analyze the gene expression profile of lung tissue in lipopolysaccharide (LPS)-induced ALI rats following treatment with human umbilical cord MSC (HUCMSC). Differential expression analyses, gene ontology annotation, Kyoto Encyclopedia of Genes and Genomes enrichment, protein–protein interaction network identification, and hub gene analysis were also performed. HUCMSC treatment decreased inflammatory factor production and alveolar exudates, and attenuated lung damage in LPS-induced ALI rats. The RNA-Seq data indicated that HUCMSC treatment activated the IL-17, JAK-STAT, NF-κB, and TNF-α signaling pathways, increased oxygen transport, and decreased extracellular matrix organization. HUCMSC exert beneficial effects on ALI via these signaling pathways by reducing inflammation, inhibiting pulmonary fibrosis, and improving lung ventilation. Moreover, our study further revealed the hub genes (Tbx2, Nkx2-1, and Atf5) and signaling pathways involved in HUCMSC treatment, thus providing novel perspectives for future research into the molecular mechanisms underlying cell treatment of ALI. HUCMSC can regulate multiple genes and signaling pathways, which can prevent LPS-induced lung damage in an ALI rat model.

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Multiple strategies enhance the efficacy of MSCs transplantation for spinal cord injury

Pang¹,

Deng²,

Zhang³

et al. 2023

Biomedicine & Pharmacotherapy

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hucMSCs transplantation promotes locomotor function recovery, reduces apoptosis and inhibits demyelination after SCI in rats

Cited by 5 publications

References 40 publications

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

RNA-Sequencing approach for exploring the therapeutic effect of umbilical cord mesenchymal stem/stromal cells on lipopolysaccharide-induced acute lung injury

Multiple strategies enhance the efficacy of MSCs transplantation for spinal cord injury

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