Hub genes and sub‐networks of stoma‐related genes in
            <i>Hylocereus undatus</i>
            through trypsin treatment during storage revealed by transcriptomic analysis

Li, Xin; Li, Bairu; Guan, Suixia; Cai, Luning; Pang, Xinyue

doi:10.1111/jfbc.13538

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…The samples of the trypsin group and control group stored for 1 week were taken for transcriptome sequencing. All process flows are carried out according to the reports of Xia et al (2017) and us (Li, Li, et al, 2021; Pang et al, 2021). The transcriptome was subjected to differential expression analysis using DESeq2 software with default parameters to find DEGs between two samples using a strict algorithm: |log 2 FC| > = 1 & p ‐adjust .05.…”

Section: Methodsmentioning

confidence: 99%

“…Our laboratory found that trypsin has a good fresh‐keeping effect on H. undatus . It can regulate the level of endogenous reactive oxygen species (ROS) in H. undatus peels (Li et al, 2019, 2020; Pang et al, 2020) and inhibit the level of saturated fatty acids (Li et al, 2022; Pang et al, 2022), induce the stoma closure (Li, Li, et al, 2021), and the biosynthesis of related substances in phenylpropanoid pathways such as lignin, chlorogenic acid (Li, Zhang, et al, 2021), and flavonoids (Pang et al, 2021). It can also affect the free radical scavenging activity of flavonoids, such as DPPH and ABTS (Li et al, 2014, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analyses and virus‐induced gene silencing identify HuWRKY40 acting as a hub transcription factor in the preservation of Hylocereus undatus by trypsin

Zhang

et al. 2022

Journal of Food Biochemistry

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Trypsin can significantly improve the storage quality of Hylocereus undatus (H. undatus). To verify the hub WRKY gene of H. undatus in trypsin preservation, joint analysis of transcriptome and protein–protein interaction (PPI) network was carried out, and virus‐induced gene silencing (VIGS) was conducted. In the transcriptome of H. undatus, GO directed acyclic graph (DAG) showed that the GO terms of 55 WRKY genes were mainly enriched in sequence‐specific DNA binding, DNA binding transcription factor activity, and so on. The GO enrichment chord diagram showed that HuWRKY40 was significantly up‐regulated in the enriched top10 GO terms. KEGG enrichment analysis showed that 55 WRKY genes were mainly enriched in plant–pathogen interaction and MAPK pathway. The results of PPI network showed that HuWRKY40 was a hub protein of WRKY transcription factors (TFs) family regulated by trypsin, which was consistent with the results of transcriptome analysis. Bioinformatics analysis showed that HuWRKY40 of H. undatus had the highest homology with Beta vulgaris L. and Spinacia oleracea L. The function of the core regulatory protein HuWRKY40 was further clarified by VIGS technology. The results of VIGS showed that there was a big difference between the phenotype of the pTRV2‐HuWRKY40 group and that of the control group. Finally, it was confirmed that HuWRKY40 accelerated the synthesis of flavonoids and improved the fruit quality during the storage of H. undatus. This study found that trypsin may regulate HuWRKY40 activity through the MAPK cascade pathway, affect the participation of flavonoid synthesis, and then delay fruit corruption. Practical applications With attention of people to the safety and freshness of fruits and vegetables, biological preservation technology has become one of the hotspots in the field of preservation in recent years. Trypsin can significantly improve the antioxidant capacity of fruits and vegetables. As a new biological preservative, it is convenient to operate and economical. In the current work, the mechanism of trypsin on the WRKY TFs during H. undatus storage was investigated. The application of trypsin would provide a new strategy for the storage quality control of fruits and vegetables.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analyses and virus‐induced gene silencing identify HuWRKY40 acting as a hub transcription factor in the preservation of Hylocereus undatus by trypsin

Zhang

et al. 2022

Journal of Food Biochemistry

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“…This gene can be targeted for genetic modifications to create a guava cultivar with good keeping quality. During storage of dragon fruits, superoxide scavengers such as trypsin have been shown to delay fruit ripening by influencing genes involved in the flavanoid pathway [ 176 ], antioxidant signal pathways [ 177 , 178 ], redox enzymes [ 179 ] and stoma closure [ 180 ], with catechin gallate being the significant metabolite regulated by the treatment. After harvest, durian fruits undergo softening and dehiscence occurs in the peel.…”

Section: Shelf Lifementioning

confidence: 99%

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Mathiazhagan

Chidambara

Laxman

et al. 2021

Genes

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The breeding of tropical fruit trees for improving fruit traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic and abiotic factors, as well as a lack of good genomic resources. Many molecular techniques have recently evolved to assist and hasten conventional breeding efforts. Molecular markers linked to fruit development and fruit quality traits such as fruit shape, size, texture, aroma, peel and pulp colour were identified in tropical fruit crops, facilitating Marker-assisted breeding (MAB). An increase in the availability of genome sequences of tropical fruits further aided in the discovery of SNP variants/Indels, QTLs and genes that can ascertain the genetic determinants of fruit characters. Through multi-omics approaches such as genomics, transcriptomics, metabolomics and proteomics, the identification and quantification of transcripts, including non-coding RNAs, involved in sugar metabolism, fruit development and ripening, shelf life, and the biotic and abiotic stress that impacts fruit quality were made possible. Utilizing genomic assisted breeding methods such as genome wide association (GWAS), genomic selection (GS) and genetic modifications using CRISPR/Cas9 and transgenics has paved the way to studying gene function and developing cultivars with desirable fruit traits by overcoming long breeding cycles. Such comprehensive multi-omics approaches related to fruit characters in tropical fruits and their applications in breeding strategies and crop improvement are reviewed, discussed and presented here.

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Trypsin preservation: CsUGT91C1 regulates Trilobatin Biosynthesis in Cucumis sativus during Storage

et al. 2023

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Hub genes and sub‐networks of stoma‐related genes in Hylocereus undatus through trypsin treatment during storage revealed by transcriptomic analysis

Cited by 3 publications

References 36 publications

Transcriptome analyses and virus‐induced gene silencing identify HuWRKY40 acting as a hub transcription factor in the preservation of Hylocereus undatus by trypsin

Transcriptome analyses and virus‐induced gene silencing identify HuWRKY40 acting as a hub transcription factor in the preservation of Hylocereus undatus by trypsin

Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content

Trypsin preservation: CsUGT91C1 regulates Trilobatin Biosynthesis in Cucumis sativus during Storage

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