Huangjinya Black Tea Alleviates Obesity and Insulin Resistance via Modulating Fecal Metabolome in High‐Fat Diet‐Fed Mice

Abstract

Scope: Huangjinya is a light-sensitive tea mutant containing low levels of tea polyphenols. Currently, most studies focused on characteristics formation, free amino acid metabolism and phytochemical purification. The biological activity of Huangjinya black tea (HJBT) on metabolic syndrome regarding fecal metabolome modulation is unavailable and is studied herein. Methods and results: High-fat diet (HFD)-fed mice are treated with HJBT for 9 weeks, various metabolic biomarkers and fecal metabolites are determined. HJBT reduces adipogenic and lipogenic gene expression, enhances lipolytic gene expression, decreases adipocyte expansion, and prevents the development of obesity. HJBT reduces lipogenic gene expression, increases fatty acid oxidation-related genes expression, which alleviates liver steatosis. HJBT enhances glucose/insulin tolerance, increases insulin/Akt signaling, attenuates hyperlipidemia and hyperglycemia, prevents the onset of insulin resistance. HJBT modulates bile acid metabolism, promotes secondary/primary bile acid ratio; increases short-chain fatty acids production, promotes saturated and polyunsaturated fatty acids content; reduces carnitines and phosphocholines, but increases myo-inositol content; decreases branched-chain and aromatic amino acids content; increases the metabolite content related to pentose phosphate pathway. Conclusion: This study reported the association between fecal metabolome modulation and metabolism improvement due to HJBT administration, proposes HJBT as a dietary intervention for preventing obesity and metabolic disorders.

Metabolomic signatures for liver tissue and cecum contents in high-fat diet-induced obese mice based on UHPLC-Q-TOF/MS

“…In the study, we used female mice in order to discover different outcomes in males based on their different hormonal profile. The results shared some similarities with previous studies that compared to the lean mice, a high-fat increased lipid profiles in the liver [ 12 ], and total bile acids (BAs) were enhanced in fecal metabolites in obese male mice [ 66 ]. However, the result was not in consistent with the finding that glycerol 3-phosphate and choline were significantly reduced [ 22 ], and CA was significantly elevated in the liver of male mice [ 54 ].…”

“…Most studies used male mice to construct high-fat diet-induced obesity model [ 12 , 65 , 66 ]. In the study, we used female mice in order to discover different outcomes in males based on their different hormonal profile.…”

Effects of Keemun and Dianhong Black Tea in Alleviating Excess Lipid Accumulation in the Liver of Obese Mice: A Comparative Study

“…The effects of black tea on fatty liver prevention in HFD-induced obese animals are well-established (18). Significant differences in chemical compositions are evident among categories of black tea; however, few studies have been conducted to systematically clarify the differences of chemical profiles and the potential health benefits of types of black tea.…”

Chickpea Extract Ameliorates Metabolic Syndrome Symptoms via Restoring Intestinal Ecology and Metabolic Profile in Type 2 Diabetic Rats

“…[14] Fortunately, plants and their extracts can reduce body weight and improve insulin resistance by enriching specific intestinal bacteria and promoting the production of microbial-derived metabolites. [15,16] However, reports on chickpea extract (CE) in this regard are limited. The purpose of the present work was to establish a T2DM model induced by HFD and streptozotocin to elaborate the effects of chickpeas on intestinal flora and metabolomics.…”