Huaier restrains proliferative and invasive potential of human hepatoma SKHEP-1 cells partially through decreased Lamin B1 and elevated NOV

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related mortality worldwide. It is urgently needed to develop potential drugs with good efficacy and low toxicity for HCC treatment. The anti-tumor effect of Traditional Chinese Medicine (TCM) has received increasing attention worldwide. Trametes robiniophila Murr. (Huaier) has been used in TCM for approximately 1,600 years. Clinically, Huaier has satisfactory therapeutic effects in cancer treatment, especially in HCC. However, the mechanisms underlying the anti-cancer effect of Huaier remain ill defined. Herein we have demonstrated that Huaier dramatically inhibited cell proliferation and induced apoptosis in human hepatoma cell line SKHEP-1. Importantly, Huaier restrained the metastatic capability of SKHEP-1 cells. Mechanistically, down-regulation of Lamin B1 and up-regulation of Nephroblastoma overexpressed (NOV) were at least partially responsible for the inhibitory effect of Huaier on the proliferative and invasive capacity of SKHEP-1 cells. Our finding provided new insights into mechanisms of anti-HCC effect of Huaier and suggested a new scientific basis for clinical medication.

An immune-stimulating proteoglycan from the medicinal mushroom Huaier up-regulates NF-κB and MAPK signaling via Toll-like receptor 4

“…(Huaier) is a medicinal fungus that has been applied for disease treatment for more than 1600 years in China. Numerous studies have shown that Huaier exhibits good clinical efficacy in the treatment of liver cancer, gastric cancer, prostate cancer, colon cancer, breast cancer, and lung cancer (1)(2)(3)(4). There is evidence that some polysaccharides of Huaier aqueous extract show anti-tumor and immunomodulatory activities.…”

“…We demonstrated that the supernatants from murine macrophage-like RAW264.7 cells treated with TPG-1 showed dramatic cytotoxicity to human hepatoma HepG2 and SK-HEP-1 cells. Moreover, TPG-1 promoted the secretion of NO, 4 tumor necrosis factor ␣ (TNF␣), and interleukin-6 (IL-6) through TLR4-dependent activation of NF-B and MAPK signaling pathways in murine macrophage-like RAW264.7 cells. Furthermore, TPG-1 treatment significantly blunted the tumorigenic ability of HepG2 or H22 cells likely partially through facilitating the infiltration of leukocytes into tumors in mice.…”

Huaier suppresses proliferative and metastatic potential of prostate cancer PC3 cells via downregulation of Lamin�B1 and induction of autophagy

“…Moreover, Lamin B1 positively regulates the proliferation and invasion of pancreatic cancer cells (25). Our previous study revealed that as a pro-oncogenic gene, Lamin B1 was dramatically decreased in human hepatoma SKHEP-1 cells exposed to Huaier (10). Intriguingly, qRT-PCR and western blot analysis demonstrated that Huaier aqueous extract significantly inhibited the mRNA and protein levels of Lamin B1 in PC3 cells in a dose-dependent manner ( Fig.…”

“…In vitro scratch assay. The scratch assay was performed as described previously (10,18). In brief, PC3 cells were seeded into 12-well plates with complete medium.…”

“…(Huaier) has a long history of disease treatment for more than 1,600 years in China. Numerous studies have revealed that Huaier exhibits superior effects for the treatment of several types of cancers, such as hepatocellular carcinoma, breast, ovarian, lung and prostate cancer (9)(10)(11)(12)(13)(14). The underlying mechanisms of the anticancer effects of Huaier include the inhibition of tumor cell proliferation, metastasis and angiogenesis, as well as induction of apoptosis (11,13,(15)(16)(17).…”