HTLV-Iii Antibody Screening of Blood Bank Donors

Carlson, James R.; Hinrichs, Steven H.; Levy, Mark J.; Yee, JoAnn; Bryant, Martin L.; Yamamoto, Janet K.; Gardner, Murray B.; Higgins, Joanne; Peterson, Nyles G.; Holland, Paul V.; Ala, F.; Collins, Ann K.; Contreras, Marcela; Entwistle, C. C.; Fraser, I.D.; Harrison, John P.; Lee, D.; Napier, John A.; Smith, Donna S.; Tovey, L. A. D.; Wagstaff, W.; Brookes, E.; McClelland, D. B. L.; Mitchell, R.; Urbaniak, S. J.; Whitrow, W.; Perry, Rachel; Cash, J. D.

doi:10.1016/s0140-6736(85)92127-0

Cited by 10 publications

(2 citation statements)

References 4 publications

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“…According to the screening principles in the methods used, tubes with a P/N ratio of 2 or greater are significantly positive [38]. However, a significant positive signal can also be higher than the mean at ?2SD [39].…”

Section: Results From Lc-elisamentioning

confidence: 99%

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Epitopic Peptides Identified by LC–ELISA and LC–MS

Zhou

Wang

2011

Chromatographia

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A new strategy of a liquid chromatography (LC)-enzyme linked immunosorbent assay (ELISA) combined with liquid chromatography-mass spectrometry (LC-MS) was developed to identify the epitopic peptides for the antigen in Streptococcus suis. Metallendopeptidase, a potential antigen for S. suis, was digested by trypsin. The tryptic solution was separated by LC under optimal condition and collected into tubes. After it was dried using a cooling vacuum, the immunogenecity of peptides in each tube was investigated by ELISA against its corresponding antibody. All peptides in each tube with positive signals based on ELISA results were identified by LC-MS. Accordingly, six putative peptides of metallendopeptidase were found and synthesized, all of which possessed high antigenicity recognized by the antibody.

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Section: Results From Lc-elisamentioning

confidence: 99%

“…All ELISA experiments were done in triplicate. The positive signal of putative peptide was defined as a signal of immunized serum/pre-serum [(P/N) C 2.0], as described by Carlson [38]; with little changing, the signal of immunized serum C mean of pre-serum ? 2SD was described by McLaughlin [39].…”

Section: Elisa Proceduresmentioning

confidence: 99%

Epitopic Peptides Identified by LC–ELISA and LC–MS

Zhou

Wang

2011

Chromatographia

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AIDS Serology Testing in Low- and High-Risk Groups

Carlson

Bryant

Hinrichs

et al. 1985

JAMA

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The performance characteristics of the acquired immunodeficiency syndrome (AIDS)-retrovirus serological tests including enzyme-linked immunosorbent assay (ELISA), Western blot, and immunofluorescence assay were defined in a clinical laboratory setting by testing 1,257 serum specimens from low- and high-risk groups for AIDS. The three prototype AIDS retroviruses (lymphadenopathy-associated virus, human T-lymphotropic virus III, and AIDS-associated retrovirus) were equally suitable as target antigen for these assays. Sera from six of 74 laboratory and health care personnel and 91 of 1,014 unselected blood donors were falsely positive by ELISA (positive to negative ratio [P/N], greater than or equal to 2) based on the lack of Western blot confirmation. Only two true-positives (two [0.2%] of 1,014 blood donors) were detected in these low-risk groups. In contrast, 106 of 108 specimens with ELISA P/N ratios of 2 or greater from the high-risk groups including asymptomatic homosexual men, hemophiliacs, AIDS-related complex patients, and AIDS patients were positive by Western blot and immunofluorescence assay. Four false-negative ELISA results based on positive immunofluorescence assay and Western blot were found in the AIDS patient group. Ten of 69 AIDS patients were negative by all three serological tests. The consequence of maintaining high sensitivity for the ELISA (P/N ratio, greater than or equal to 2) as a screening test was a loss of specificity. The number of false-positive results necessitated the use of a confirmation test with greater specificity.

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