HTLV‐1 provirus load in peripheral blood lymphocytes of HTLV‐1 carriers is diminished by green tea drinking

Sonoda, Junichiro; Koriyama, Chihaya; Yamamoto, Shinako; Kozako, Tomohiro; Li, Hong Chuan; Lema, Carolina; Yashiki, Shinji; Fujiyoshi, Toshinobu; Yoshinaga, Mitsuhiro; Nagata, Yukihiro; Akiba, Suminori; Takezaki, Toshiro; Yamada, Katsushi; Sonoda, Shunro

doi:10.1111/j.1349-7006.2004.tb02493.x

Cited by 40 publications

(32 citation statements)

References 31 publications

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“…HTLV-1 proviral DNA was measured by the quantitative PCR amplification of HTLV-1 pX DNA. 10 The detection limit of the HTLV-1 proviral load was 0.5 copies per 1000 cells. The OS curve was estimated by the Kaplan-Meier method.…”

Section: Methodsmentioning

confidence: 99%

Long-term outcomes after hematopoietic SCT for adult T-cell leukemia/lymphoma: results of prospective trials

Choi

Tanosaki²,

Uike

et al. 2010

Bone Marrow Transplant

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Section: Methodsmentioning

confidence: 99%

Long-term outcomes after hematopoietic SCT for adult T-cell leukemia/lymphoma: results of prospective trials

Choi

Tanosaki²,

Uike

et al. 2010

Bone Marrow Transplant

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“…The HTLV-1 proviral load in PBMC was assayed in 64 of 67 subjects by methods of quantitative PCR using a Light Cycler System (Roche Diagnostics) by intraassay using a series of duplicate measurements of 12 test samples with standard DNA of four different dilutions for each assay as described previously (43). In brief, the duplicate intraassay for HTLV-1 provirus load in PBMC was run by simultaneous measurements of ␤-globin DNA and HTLV-1 DNA using the standard DNA, ␤-globin DNA from Roche Diagnostics, and HTLV-1 provirus DNA from MT-2 cells.…”

Section: Real-time Pcr Quantification Of Htlv-1 Proviral Load In Pbmcmentioning

confidence: 99%

Reduced Frequency, Diversity, and Function of Human T Cell Leukemia Virus Type 1-Specific CD8+ T Cell in Adult T Cell Leukemia Patients

Kozako

Arima

Toji

et al. 2006

The Journal of Immunology

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Human T cell lymphotropic virus type 1 (HTLV-1)-specific CTL are thought to be immune effectors that reduce the risk of adult T cell leukemia (ATL). However, in vivo conditions of anti-HTLV-1 CTL before and after ATL development have yet to be determined. To characterize anti-HTLV-1 CTL in asymptomatic HTLV-1 carriers (AC) and ATL patients, we analyzed the frequency and diversity of HTLV-1-specific CD8+ T cells in PBMC of 35 AC and 32 ATL patients using 16 distinct epitopes of HTLV-1 Tax or Env/HLA tetramers along with intracellular cytolytic effector molecules (IFN-γ, perforin, and granzyme B). Overall frequency of subjects possessing Tax-specific CD8+ T cells was significantly lower in ATL than AC (53 vs 90%; p = 0.001), whereas the difference in Env-specific CD8+ T cells was not statistically significant. AC possessed Tax11–19/HLA-A*0201-specific tetramer+ cells by 90% and Tax301–309/HLA-A*2402-specific tetramer+ cells by 92%. Some AC recognized more than one epitope. In contrast, ATL recognized only Tax11–19 with HLA-A*0201 and Tax301–309 with HLA-A*2402 at frequencies of 30 and 55%. There were also significant differences in percentage of cells binding Tax11–19/HLA-A*0201 and Tax301–309/HLA-A*2402 tetramers between AC and ATL. Anti-HTLV-1 Tax CD8+ T cells in AC and ATL produced IFN-γ in response to Tax. In contrast, perforin and granzyme B expression in anti-HTLV-1 CD8+ T cells of ATL was significant lower than that of AC. Frequency of Tax-specific CD8+ T cells in AC was related to proviral load in HLA-A*0201. These results suggest that decreased frequency, diversity, and function of anti-HTLV-1 Tax CD8+ T cell clones may be one of the risks of ATL development.

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“…Human T-cell leukemia caused by human T-cell lymphotropic virus type-1 (HTLV-1) may also be treated with EGCG as well as green tea extract as a whole (Araya et al 2011). Green tea is reported to reduce the HTLV-1 provirus in peripheral blood lymphocytes in the HTLV-1 carrier (Sonoda et al 2004), that downregulates the protein expression of an anti-apoptotic member (Harakeh et al 2008). Recent studies have shown that (-)-epigallocatechin gallate (Figure 1) is a major compound to norovirus (Ryu et al 2015).…”

Section: Feline Calicivirus (Fvs) a Surrogate Of Norovirus And Other mentioning

confidence: 99%

Antiviral effects of green tea (<i>Camellia sinensis</i>) against pathogenic viruses in human and animals (a mini-review)

Mahmood¹,

Martínez²,

Aslam³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: Tea is the second most addictive worldwide after formulations containing caffeine in carbonated beverage. Green tea is made from leaves of the Camellia sinensis plant. In the repertoire of traditional Chinese medicine, green tea beverages have played a fundamental role associated with their culture. It has been suggested that green tea has a number of positive health benefits that are reviewed and discussed in this minireview. Materials and Methods:We performed a search using the key words "green tea" AND "antiviral" covering the last 10 years. The consulted data based were PubMed, ISI Web of Knowledge, Scopus, Reuters and Thomson. Results:The results of the searching greatly support that green tea presents both antibacterial and antiviral effects. The beneficial effects of green tea are mainly attributed to the presence of a type of polyphenols known as catechins and formed by several isomers including (-) -epigallocatechin gallate (EGCG), (-) -epigallocatechin, (-) -epicatechin gallate, (-) -epicatechin, and (+) -catechin. The catechins in green tea have a wide range of antiviral activity against a variety of viruses that act by interfering with its replication cycle. Conclusion: A detailed information on the antiviral activity of green tea in a number of different viruses show a promising future as a popular drink and also as a potential therapeutic agent.

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HTLV‐1 provirus load in peripheral blood lymphocytes of HTLV‐1 carriers is diminished by green tea drinking

Cited by 40 publications

References 31 publications

Long-term outcomes after hematopoietic SCT for adult T-cell leukemia/lymphoma: results of prospective trials

Long-term outcomes after hematopoietic SCT for adult T-cell leukemia/lymphoma: results of prospective trials

Reduced Frequency, Diversity, and Function of Human T Cell Leukemia Virus Type 1-Specific CD8+ T Cell in Adult T Cell Leukemia Patients

Antiviral effects of green tea (<i>Camellia sinensis</i>) against pathogenic viruses in human and animals (a mini-review)

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