HTLV-1 Provirus and Mycosis Fungoides

Bazarbachi, Abdul-Hamid; Saal, F; Laroche, Liliane; Flageul, B.; Périès, J.; The, H de

doi:10.1126/science.8451646

Cited by 26 publications

(15 citation statements)

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“…Although these findings are consistent with those of some groups, they contrast with those of others (Table I). Among possible explanations, that of false negative results in this and other studies (Abrams et al, 1991;Bazarbachi et al, 1993;Capesius et al, 1991;Lisby et al, 1992;Moudgil et al, 1994;Pombo de Oliviera et al, 1995;Vallejo et al, 1995) seems unlikely. The sensitivity of techniques used here was equal to or greater than that of other groups due to the use of nested PCR and a PCR-based RT assay.…”

Section: Discussionmentioning

confidence: 55%

“…Lisby et al (1992) were unable to detect HTLV-I DNA sequences by PCR in punch skin biopsies from patients with CTCL. In 1993 we found no HTLV-I sequences in cutaneous lesions from 18 French and Portuguese MF and SS patients, even when PCR was performed using HTLV-I gag, pol, env, pX and LTR primers (Bazarbachi et al, 1993). In light of these data, a co-operative international study was set up to further investigate this issue on a large series of patients with different geographical backgrounds.…”

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confidence: 95%

“…Association between mycosis fungoides (MF) (Patterson & Edelson, 1985), its leukaemic variant Sezary syndrome (SS) and the human T-cell lymphotropic virus type I (HTLV-I) (Poiesz et al, 1980) has been controversial, as the reported incidence of infection has varied between 0% and nearly 100% (see Table I) (Abrams et al, 1991;Anagnostopoulos et al, 1990;Bazarbachi et al, 1993;Capesius et al, 1991;Chan et al, 1993;D'Incan et al, 1990;Dave et al, 1995;Fouchard et al, 1995;Ghosh et al, 1994;Hall et al, 1991;Lisby et al, 1992;Manca et al, 1994;Moudgil et al, 1994;Pancake et al, 1995;Pombo de Oliviera et al, 1995;Srivastava et al, 1992;Vallejo et al, 1995;Whittaker & Luzatto, 1993;ZuckerFranklin et al, 1991ZuckerFranklin et al, , 1992. HTLV-I is the aetiologic agent of adult T-cell leukaemia/lymphoma (ATLL) and the majority of patients with this disease are HTLV-I seropositive with clonal integration of the HTLV-I provirus in the leukaemic cells (Takatsuki et al, 1985).…”

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Mycosis fungoides and Sezary syndrome are not associated with HTLV‐I infection: an international study

et al. 1997

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Summary. Association between mycosis fungoides (MF), its leukaemic variant Sezary syndrome (SS) and the human Tcell lymphotropic virus type-I (HTLV-I) has been controversial, with the reported incidence of infection varying between 0% and nearly 100%. We studied 127 patients (85 MF, 28 SS, five Sezary cell leukaemia, four lymphomatoid papulosis, and five unspecified cutaneous T-cell lymphomas (CTCL)) originating from Europe (France, Spain, U.K., Portugal) or from U.S.A. (California) for the presence of HTLV-I infection markers. HTLV-I and -II serology were performed on 78 patients using standard immunological methods. Reverse transcriptase (RT) assay was also performed in 26 cases using an RT-PCR-based method of high sensitivity. Molecular analyses were performed on 215 DNA samples (121 from fresh PBMCs, 26 from PBMCs after short-term culture and 68 from skin lesions) by PCR amplification using HTLV-I and -II gag, pol, env, pX and LTR specific primers.Immunological tests were negative except for two sera which were indeterminate. PCR with all HTLV-I and -II primer pairs showed negative results in all 215 samples investigated. No RT activity was detected in short-term PBMC cultures of any of the 26 cases studied. The results of this large study from five different countries clearly indicate that MF and SS are not associated with HTLV-I infection.

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Mycosis fungoides and Sezary syndrome are not associated with HTLV‐I infection: an international study

et al. 1997

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“…It has taken the development of the PCR and the use of a variety of primers and probes to uncover evidence of HTLV infection in the majority of MF patients. It is not clear why our efforts were more successful than those of others ( 11,(52)(53)(54) in showing a high incidence of HTLV in MF patients, many of whom are Caucasian and do not originate from HTLV-I-endemic areas (see Table I). Part of the reason for the inconsistent detection of HTLV sequences is likely to be due to the low levels of virus or to the presence of incomplete or variant proviral sequences in the samples studied.…”

Section: Resultsmentioning

confidence: 81%

The cutaneous T cell lymphoma, mycosis fungoides, is a human T cell lymphotropic virus-associated disease. A study of 50 patients.

Pancake

Zucker‐Franklin²,

Coutavas³

1995

J. Clin. Invest.

138

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For nearly two decades it has been suspected that the cutaneous T cell lymphoma, mycosis fungoides (MF), and its leukemic variant, the Sezary syndrome, are caused by the human T lymphotropic virus (HTLV-I/ll). Arguments against this concept included the finding that only a small number of MF patients have antibodies to HTLV-I/ll and that attempts to detect proviral sequences by mere Southern hybridization of extracted DNA usually met with failure. However, we have reported repeatedly that HTLV-like particles emerge in blood mononuclear cell (PBMC) cultures of practically all patients with this disease. In several instances, the particles were identified as HTLV by immunoelectron microscopy as well as biomolecular analysis. With the assumptions that the virus in MF patients may have become defective and that the remaining signal may be too weak for detection by Southern hybridization alone, the extracts of freshly isolated PBMC of 50 consecutive patients were subjected to combined PCR/Southern analysis. Here we report the presence of HTLV pol and/or tax proviral sequences in 46 out of 50 (92%) of the patients tested. In addition, five of the patients, who lacked antibodies to HTLV-I/ll structural proteins, were found to be seropositive for tax. It thus seems reasonable to conclude that MF/ Sezary syndrome is an HTLV-associated disease and that lack of an immune response does not preclude infection with this type of virus. (J. Clin. Invest. 1995. 95:547-554.)

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“…35 In contrast, other groups failed to detect such HTLV-I sequences in similar patients. 19,59 On the other hand, the presence of defective HTLV-I provirus was detected in four patients suffering from CD30 anaplastic large cell CTCL. 21 In the present study, all the CTCL other than ATL cases, were HTLV-I-seronegative and we were unable to detect any specific HTLV-I sequences by PCR in the DNA extracted from PBMC or cutaneous lesions of the SS, MF or pleomorphic CTCL.…”

Section: Discussionmentioning

confidence: 99%

Cutaneous T cell lymphomas: mycosis fungoides, Sezary syndrome and HTLV-I-associated adult T cell leukemia (ATL) in Mali, West Africa: a clinical, pathological and immunovirological study of 14 cases and a review of the African ATL cases

et al. 1998

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Cutaneous T cell lymphomas (CTCL) are rare lymphoproliferative diseases, which are frequently suspected to be of viral origin. As very few data were available concerning cutaneous T cell lymphomas in tropical Africa, we undertook a clinical, histopathological, immunological and viro-molecular study of patients with a clinical diagnosis of cutaneous lymphoma, in Bamako, Mali. While prior to this study, no case of CTCL had been reported in this country, 14 patients (five women, nine men; mean age 58 years) with a diagnosis of cutaneous lymphoma were seen over a period of 30 months (1992-1994) in the only dermatological department in Mali. Clinically, the most frequent pattern was an infiltrated erythrodermia similar to Sezary syndrome. Nodular lesions and/or plaques were rarely observed. All these cutaneous tumors were T cell lymphoproliferations, only one expressing the CD8 + antigen. A comprehensive analysis of all the available data permitted characterization of three cases of adult T cell leukemia/lymphoma (ATL) associated with HTLV-I (one definitive case, of leukemic type, with demonstration of clonal integration of HTLV-I proviral genome and two probable ATL cases), three cases of Sezary syndrome (SS), two cases of mycosis fungoides (MF) and five cases of pleomorphic cutaneous lymphoma. In one case, the differentiation between MF and pleomorphic cutaneous lymphoma could not be established. HTLV-I serological and/or molecular markers were restricted to the three ATL cases. From the unique definitive ATL case, a T cell line was established from culture of peripheral blood mononuclear cells and sequence analysis of the env gene and the U3-LTR region demonstrated that the virus present in this patient belonged to the cosmopolitan subtype A. Thus, we report here the first evidence of HTLV-I infection and associated ATL in Mali. This is the second ATL case described for the whole Sahelian region (one ATL of the lymphoma type was reported previously in a Mauritanian patient). Furthermore, we demonstrate that the main types of CTCL described in Europe and North America are also present in this African area and that the prevalence of these diseases is greatly underestimated in such regions. Furthermore, no association was observed between HTLV-I/II infection and SS, MF or pleomorphic cutaneous lymphoma in Mali in contrast to other studies.

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HTLV-1 Provirus and Mycosis Fungoides

Cited by 26 publications

References 2 publications

Mycosis fungoides and Sezary syndrome are not associated with HTLV‐I infection: an international study

Mycosis fungoides and Sezary syndrome are not associated with HTLV‐I infection: an international study

The cutaneous T cell lymphoma, mycosis fungoides, is a human T cell lymphotropic virus-associated disease. A study of 50 patients.

Cutaneous T cell lymphomas: mycosis fungoides, Sezary syndrome and HTLV-I-associated adult T cell leukemia (ATL) in Mali, West Africa: a clinical, pathological and immunovirological study of 14 cases and a review of the African ATL cases

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