HSP90AB1 is a host factor that promotes porcine deltacoronavirus replication

Zhao, Yujia; Yuan, Jianlin; Xiao, Dai; Zhang, Luwen; Li, Cheng; Hu, Jingfei; Chen, Rui; Song, Daili; Wen, Yiping; Wu, Rui; Zhao, Qin; Du, Senyan; Yan, Qigui; Han, Xinfeng; Wen, Xintian; Cao, Sanjie; Huang, Xiaobo

doi:10.1016/j.jbc.2023.105536

Cited by 5 publications

(1 citation statement)

References 55 publications

(73 reference statements)

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“…JEV internalization assays were carried out by incubating JEV with C6/36 cells at 4°C for 1 h to allow JEV binding, after which the unbound JEV virions were removed by rapidly washing three times with PBS. Then, the cells were transferred to 30°C for 1 h to allow JEV internalization and treated with sodium citrate buffer (pH3.0) to remove noninternalized virions ( 78 , 79 ). Total RNA was extracted, and the viral mRNA levels were determined by RT-qPCR.…”

Section: Methodsmentioning

confidence: 99%

Sialic acids as attachment factors in mosquitoes mediating Japanese encephalitis virus infection

He,

Miao,

Yang

et al. 2024

J Virol

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The role of Culex mosquitoes in the transmission of Japanese encephalitis virus (JEV) is crucial, yet the mechanisms of JEV infection in these vectors remain unclear. Previous research has indicated that various host factors participate in JEV infection. Herein, we present evidence that mosquito sialic acids enhance JEV infection both in vivo and in vitro . By treating mosquitoes and C6/36 cells with neuraminidase or lectin, the function of sialic acids is effectively blocked, resulting in significant inhibition of JEV infection. Furthermore, knockdown of the sialic acid biosynthesis genes in Culex mosquitoes also leads to a reduction in JEV infection. Moreover, our research revealed that sialic acids play a role in the attachment of JEV to mosquito cells, but not in its internalization. To further explore the mechanisms underlying the promotion of JEV attachment by sialic acids, we conducted immunoprecipitation experiments to confirm the direct binding of sialic acids to the last α-helix in JEV envelope protein domain III. Overall, our study contributes to a molecular comprehension of the interaction between mosquitoes and JEV and offers potential strategies for preventing the dissemination of flavivirus in natural environments. IMPORTANCE In this study, we aimed to investigate the impact of glycoconjugate sialic acids on mosquito infection with Japanese encephalitis virus (JEV). Our findings demonstrate that sialic acids play a crucial role in enhancing JEV infection by facilitating the attachment of the virus to the cell membrane. Furthermore, our investigation revealed that sialic acids directly bind to the final α-helix in the JEV envelope protein domain III, thereby accelerating virus adsorption. Collectively, our results highlight the significance of mosquito sialic acids in JEV infection within vectors, contributing to a better understanding of the interaction between mosquitoes and JEV.

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Section: Methodsmentioning

confidence: 99%