HSP90 as a novel therapeutic target for posterior capsule opacification

Li, Jing; Xue, Wenxian; Wang, Xuance; Huang, Weikang; Wang, Xiangxiang; Li, Hui; Cui, Xiukun; Li, Mengyuan; Mu, Hongmei; Ren, Yanzhu; Zhang, Fengyan; Hu, Yanzhong

doi:10.1016/j.exer.2019.107821

Cited by 17 publications

(18 citation statements)

References 34 publications

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“…The results of the present study showed that HSP90 was significantly expressed in the testicular tissue of diabetic rats compared with control group rats ( p < 0.001), although HSP90 is potentially important in offering cytoprotection at physiological limits [ 24 ]. But in the case where its upregulation is increased, it is expected to develop negative impacts through inhibition of production of other HSPs that lead to the cytoprotection [ 25 – 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Heat shock protein (HSP) 90 exists in cells as a chaperone that acts in controlling cellular changes such as protein refolding, removing denatured proteins through the process of the proteasome [ 24 ]. HSP90 participates in making what is called the machine of chaperone [ 24 ]. Various types of stress can induce the production of HSP90 including cancer and diabetes [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…HSP90 participates in making what is called the machine of chaperone [ 24 ]. Various types of stress can induce the production of HSP90 including cancer and diabetes [ 24 ]. Several studies pointed that the overexpression of HSP90 has negative impacts such as lowering cytoprotective induction of other HSPs including HSP40 and HSP70 through interaction with heat shock factor 1 (HSF-1) [ 25 – 28 ].…”

Section: Introductionmentioning

confidence: 99%

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The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes

Alsarhan

Amawi

Almazari

et al. 2020

Analytical Cellular Pathology

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Introduction. Diabetes is increasingly prevalent at global level and associated with various impacts including the male reproductive system. Aims. This research is aimed at investigating the influence of diabetes on the localization and expression of HSP90 and iNOS in the testicular tissue of diabetic rats. Methods. A diabetic model was developed through a single injection of alloxan monohydrate intraperitoneally (purchased from Sigma-Aldrich) 120 mg/kg body weight following fasting for 12 hrs. The experiment involved two groups, the control and diabetic groups with 10 albino rats in each group. Diabetes was considered if glucose concentration was ≥200 mg/dl. The experiment duration was for one month. After the experiment had finished, all rats were terminated and prepared for routine histological and immunohistochemical examination. Results. The results revealed that diabetes caused morphological changes at histological level in testicular tissue. Immunohistochemical examination showed that diabetes significantly upregulated the expression of both HSP90 and iNOS in the testicular tissue of diabetic rats as compared with that of the control group (p<0.001). Conclusion. Diabetes may induce adverse health effects on the male reproduction through upregulation of HSP90 and iNOS in the testicular tissue of diabetic rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes

Alsarhan

Amawi

Almazari

et al. 2020

Analytical Cellular Pathology

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“…However, its role in PCO is not known. Li et al [51] investigated the potential therapeutic use of HSP90 in PCO using an LEC line and an ex vivo rat lens capsule. Protein expression in response to application of tanespimycin (17-AAG), an HSP90 inhibitor, was assessed by immunoblotting and real-time polymerase chain reaction (RT-PCR) and apoptosis by the TdT-mediated dUTP nick-end labeling method.…”

Section: In Vitro Research Involving Photodynamic Therapymentioning

confidence: 99%

Posterior Capsule Opacification: A Review of Experimental Studies

Konopińska

Młynarczyk

Dmuchowska

et al. 2021

JCM

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Posterior capsule opacification (PCO) is the most common complication of cataract surgery. It causes a gradual deterioration of visual acuity, which would otherwise improve after a successful procedure. Despite recent advances in ophthalmology, this complication has not been eradicated, and the incidence of PCO can be as high as 10%. This article reviews the literature concerning the pathomechanism of PCO and examines the biochemical pathways involved in its formation and methods to prevent this complication. We also review the reported tests performed in cell cultures under laboratory conditions and in experimental animal models and in ex vivo human lens capsules. Finally, we describe research involving human eyes in the clinical setting and pharmacological methods that may reduce the frequency of PCO. Due to the multifactorial etiology of PCO, in vitro studies make it possible to assess the factors contributing to its complications and search for new therapeutic targets. Not all pathways involved in cell proliferation, migration, and contraction of the lens capsule are reproducible in laboratory conditions; moreover, PCO in humans and laboratory animals may be additionally stimulated by various degrees of postoperative reactions depending on the course of surgery. Therefore, further studies are necessary.

show abstract

“…However, its role in PCO is not known. Li et al (53) investigated the potential therapeutic use of HSP90 in PCO using an LEC line and an ex vivo rat lens capsule. Protein expression in response to application of tanespimycin (17-AAG), an HSP90 inhibitor, was assessed by immunoblotting and real-time polymerase chain reaction (RT-PCR) and apoptosis by the TdT-mediated dUTP nick-end labeling (TUNEL) method.…”

Section: Ex Vivo Tests With Heat Shock Protein 90mentioning

confidence: 99%

Posterior Capsule Opacification: A Review of Experimental Studies

Konopińska¹,

Romaniuk²,

Dmuchowska³

et al. 2021

Preprint

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Posterior capsule opacification (PCO) is the most common complication of cataract surgery. It causes a gradual deterioration of visual acuity, which would otherwise remain improved after a successful procedure. Despite recent advances in ophthalmology, this complication has not been eradicated and the incidence of PCO can be as high as 10%. This article reviews the literature concerning the pathomechanism of PCO and examines the biochemical pathways involved in its formation and methods to prevent this complication. We also review the reported tests performed in cell cultures under laboratory conditions, in experimental animal models, and in ex vivo human lens capsules. Finally, we describe research involving human eyes in the clinical setting and pharmacological methods that may reduce the frequency of PCO. Due to the multifactorial eti-ology of PCO, in vitro studies make it possible to assess the factors contributing to its complica-tions and search for new therapeutic targets. Not all pathways involved in cell proliferation, mi-gration, and contraction of the lens capsule are reproducible in laboratory conditions; moreover, PCO in humans and laboratory animals may be additionally stimulated by various degrees of postoperative reactions depending on the course of surgery. Therefore, further studies are necessary.

show abstract

HSP90 as a novel therapeutic target for posterior capsule opacification

Cited by 17 publications

References 34 publications

The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes

The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes

Posterior Capsule Opacification: A Review of Experimental Studies

Posterior Capsule Opacification: A Review of Experimental Studies

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