HSP90 as a global genetic modifier for male genital morphology in<i>Drosophila melanogaster</i>

Takahashi, Kazuo H.; Ishimori, Motoyuki; Iwata, Hiroyoshi

doi:10.1111/evo.13598

Cited by 7 publications

(4 citation statements)

References 54 publications

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“…We found that E5 is expressed in the posterior lobe, the ventral portion of the EVL (see additional samples online), and the phallus. E5 is a homeodomain transcription factor (Dalton et al 1989) associated with variation in posterior lobe morphology among Drosophila melanogaster populations (Takahashi et al 2018). We also found that brother of odd with entrails limited ( bowl ) is expressed in the posterior lobe at 48 hr APF, as well as other tissues throughout the terminalia (Figure 2C-E).…”

Section: Resultsmentioning

confidence: 99%

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofDrosophila melanogaster

Vincent

Rice

Wong

et al. 2019

G3 Genes|Genomes|Genetics

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During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have identified network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster male terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the male terminalia are highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (https://flyterminalia.pitt.edu/) as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.

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Section: Resultsmentioning

confidence: 99%

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofDrosophila melanogaster

Vincent

Rice

Wong

et al. 2019

G3 Genes|Genomes|Genetics

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“…Our results support the view that the role of proteostasis is but one part of an integrated "quality system (QS)" 20 that is responsive to genetics, development, aging, and the environment to continually reshape sequence-to-function-to-structure relationships driving protein fold dynamics contributing to health and disease 1,17,20,34,110 that is not captured in static structures 21,34 . As it is well established by the pioneering efforts of Lindquist and others 68,[111][112][113][114][115][116][117][118][119][120][121][122][123][124][125][126][127] that Hsp90 serves as a capacitor for natural selection and evolution, SCV principled relationships now suggest that Hsp90's role in capacitance management of variation occurs on a residue-by-residue basis-but as a collective. The response of Hsp90 family members to ATPase inhibitors suggests their role in cellular QS 20 is acutely tuned by the use of different cochaperone partners to facilitate fitness 6,14,15,44,128 .…”

Section: Discussionmentioning

confidence: 99%

Tracing genetic diversity captures the molecular basis of misfolding disease

Zhao,

Wang,

Sun

et al. 2024

Nat Commun

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Genetic variation in human populations can result in the misfolding and aggregation of proteins, giving rise to systemic and neurodegenerative diseases that require management by proteostasis. Here, we define the role of GRP94, the endoplasmic reticulum Hsp90 chaperone paralog, in managing alpha-1-antitrypsin deficiency on a residue-by-residue basis using Gaussian process regression-based machine learning to profile the spatial covariance relationships that dictate protein folding arising from sequence variants in the population. Covariance analysis suggests a role for the ATPase activity of GRP94 in controlling the N- to C-terminal cooperative folding of alpha-1-antitrypsin responsible for the correction of liver aggregation and lung-disease phenotypes of alpha-1-antitrypsin deficiency. Gaussian process-based spatial covariance profiling provides a standard model built on covariant principles to evaluate the role of proteostasis components in guiding information flow from genome to proteome in response to genetic variation, potentially allowing us to intervene in the onset and progression of complex multi-system human diseases.

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“…2011; Masly and Kamimura 2014; Frazee and Masly 2015; Takahara and Takahashi 2015; Takahashi et al. 2018; Tanaka et al. 2018).…”

Section: Methodsmentioning

confidence: 99%

Interspecific introgression reveals a role of male genital morphology during the evolution of reproductive isolation in Drosophila

et al. 2021

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Rapid divergence in genital structures among nascent species has been posited to be an early‐evolving cause of reproductive isolation, although evidence supporting this idea as a widespread phenomenon remains mixed. Using a collection of interspecific introgression lines between two Drosophila species that diverged approximately 240,000 years ago, we tested the hypothesis that even modest divergence in genital morphology can result in substantial fitness losses. We studied the reproductive consequences of variation in the male epandrial posterior lobes between Drosophila mauritiana and Drosophila sechellia and found that divergence in posterior lobe morphology has significant fitness costs on several prefertilization and postcopulatory reproductive measures. Males with divergent posterior lobe morphology also significantly reduced the life span of their mates. Interestingly, one of the consequences of genital divergence was decreased oviposition and fertilization, which suggests that a sensory bias for posterior lobe morphology could exist in females, and thus, posterior lobe morphology may be the target of cryptic female choice in these species. Our results provide evidence that divergence in genitalia can in fact give rise to substantial reproductive isolation early during species divergence, and they also reveal novel reproductive functions of the external male genitalia in Drosophila.

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HSP90 as a global genetic modifier for male genital morphology inDrosophila melanogaster

Cited by 7 publications

References 54 publications

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofDrosophila melanogaster

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofDrosophila melanogaster

Tracing genetic diversity captures the molecular basis of misfolding disease

Interspecific introgression reveals a role of male genital morphology during the evolution of reproductive isolation in Drosophila

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