HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors

Abstract

Heat shock proteins (HSPs) are a large class of highly conserved chaperons, which play important roles in response to elevated temperature and other environmental stressors. In the present study, 5 HSP90 genes and 17 HSP70 genes were systematically characterized in spotted seabass (Lateolabrax maculatus). The evolutionary footprint of HSP genes was revealed via the analysis of phylogeny, chromosome location, and gene copy numbers. In addition, the gene structure features and the putative distribution of heat shock elements (HSEs) and hypoxia response elements (HREs) in the promoter regions were analyzed. The protein-protein interaction (PPI) network analyses results indicated the potential transcriptional regulation between the heat shock factor 1 (HSF1) and HSPs and a wide range of interactions among HSPs. Furthermore, quantitative (q)PCR was performed to detect the expression profiles of HSP90 and HSP70 genes in gill, liver, and muscle tissues after heat stress, meanwhile, the expression patterns in gills under alkalinity and hypoxia stresses were determined by analyzing RNA-Seq datasets. Results showed that after heat stress, most of the examined HSP genes were significantly upregulated in a tissue-specific and time-dependent manners, and hsp90aa1.1, hsp90aa1.2, hsp70.1, and hsp70.2 were the most intense responsive genes in all three tissues. In response to alkalinity stress, 11 out of 13 significantly regulated HSP genes exhibited suppressed expression patterns. Alternatively, among the 12 hypoxia-responsive-expressed HSP genes, 7 genes showed induced expressions, while hsp90aa1.2, hsp70.1, and hsp70.2 had more significant upregulated changes after hypoxic challenge. Our findings provide the essential basis for further functional studies of HSP genes in response to abiotic stresses in spotted seabass.

Genome-Wide Identification, Molecular Characterization, and Involvement in Response to Abiotic and Biotic Stresses of the HSP70 Gene Family in Turbot (Scophthalmus maximus)

“…With increasing temperature, almost all of these genes had significantly and continuously upregulated expression, and the number of DE HSP70 genes was clearly increased. Similar results have also been found in the L. maculatus and O. mykiss , in which most of the examined HSP70 genes showed significantly upregulated expression in different tissues after exposure to heat stress [ 18 , 22 ]. Moreover, four significantly upregulated HSP70 genes in both kidney and liver tissues were identified, hsp70 ( hspa1a ), hspa1b , hspa5 , and hspa8a.1 .…”

“…Heat shock protein 70 (HSP70) gene family members are involved in various housekeeping and stress-related activities in all organisms from bacteria to mammals [ 36 , 37 ]. Due to their important roles, especially in enhancing the response of cells to various stress stimuli, the HSP70 gene family has been extensively studied in a variety of aquaculture species, including invertebrate [ 38 ], bivalve [ 39 ], and multiple teleost species [ 14 , 18 , 19 , 20 , 21 , 23 ]. Nevertheless, a systematic analysis of HSP70 genes in the turbot has been lacking.…”

“…Given the crucial functions of HSP70 genes in response to environmental stresses coupled with the great abundance of available genome sequence resources, an increasing number of HSP70 gene families have been identified at the genome-wide level in multiple teleosts, and the potential roles of these genes in response to abiotic and biotic stresses have also been described in recent years. For instance, 17 HSP70 genes were systematically characterized and analyzed in the spotted seabass ( Lateolabrax maculatus ); among them, 4, 11, and 7 gene family members were found to be involved in the response to heat, hypoxia, and alkalinity stress, respectively [ 18 ]. In the large yellow croaker ( Larimichthys crocea ), 17 HSP70 genes were identified at the genome-wide level; moreover, 6 gene family members were significantly up- or downregulated in liver tissue after exposure to cold and heat stresses, as determined with transcriptome data [ 19 ].…”

Identification of Novel Mrna Isoforms Associated with Acute Heat Stress Response Using RNA-Sequencing Data in The Sprague-Dawley Rats

“…Under H120, the gene encoding the heat shock protein 86 (Hsp86), also known as Hsp90aa1, was annotated by Gene_4216. Numerous studies have revealed the Hsp90aa1 gene can be significantly upregulated in a tissue-specific and time-dependent manner after heat stress [71]. Moreover, our previous study also confirmed the important role of HSP70AA1 in dairy cattle [72].…”

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats

“…Under H120, the gene encoding the heat shock protein 86 (Hsp86), also known as Hsp90aa1 , was annotated by Gene_4216 . Numerous studies have revealed the Hsp90aa1 gene can be significantly up-regulated in a tissue-specific and time-dependent manner after heat stress [ 71 ]. Moreover, our previous study also confirmed the important role of HSP70AA1 in dairy cattle [ 72 ].…”