HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise

Febbraio, Mark A.; Koukoulas, Irene

doi:10.1152/jappl.2000.89.3.1055

Cited by 148 publications

(143 citation statements)

References 28 publications

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“…The expression levels of PGC-1␣ and -1␤ mRNA were slightly, but significantly, elevated in the SAMP1-CatEx group. Heat shock protein 72, a stress-and exercise-inducible protein, is thought to play an important role in preventing muscle damage and maintaining protein synthesis in skeletal muscle (9,32). Heat shock protein 72 mRNA was increased by exercise and was 23% higher in the SAMP1-CatEx group than in the SAMP1-Con group.…”

Section: Body Weight and Tissue Weightsmentioning

confidence: 99%

Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice

Murase¹,

Haramizu²,

Ota³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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echins, which are abundant in green tea, possess a variety of biologic actions, and their clinical application has been extensively investigated. In this study, we examined the effects of tea catechins and regular exercise on the aging-associated decline in physical performance in senescence-accelerated prone mice (SAMP1) and agematched senescence-accelerated resistant mice (SAMR1). The endurance capacity of SAMR1 mice, measured as the running time to exhaustion, tended to increase over the 8-wk experimental period, whereas that of SAMP1 mice decreased by 17%. On the other hand, the endurance capacity of SAMP1 mice fed 0.35% (wt/wt) catechins remained at the initial level and was significantly higher than that of SAMP1 mice not fed catechins. In SAMP1 mice fed catechins and given exercise, oxygen consumption was significantly increased, and there was an increase in skeletal muscle fatty acid ␤-oxidation. The mRNA levels of mitochondria-related molecules, such as peroxisome proliferator-activated receptor-␥ coactivator-1, cytochrome c oxidase-II, III, and IV in skeletal muscle were also higher in SAMP1 mice given both catechins and exercise. Moreover, oxidative stress measured as thiobarbituric reactive substances was lower in SAMP1 groups fed catechins than in the SAMP1 control group. These results suggest that long-term intake of catechins, together with habitual exercise, is beneficial for suppressing the aging-related decline in physical performance and energy metabolism and that these effects are due, at least in part, to improved mitochondrial function in skeletal muscle. energy metabolism; exercise; green tea; mitochondria; oxidative stress MARKED DECLINE IN PHYSICAL performance with increasing age is a crucial problem in our aging society. As people age, skeletal muscle mass and strength decrease, fatty acid metabolism changes, physical activity declines, and body fat mass increases, which results in an increased incidence of lifestylerelated diseases, bed rest, and other morbidities (31). These aging-related alterations are assumed to be associated with changes in the expression and activity of muscular mitochondria-related molecules, such as cytochrome c oxidases (COXs), electron transfer chain enzymes, and peroxisome proliferatoractivated receptor-␥ coactivator-1 (PGC-1), which mediate mitochondrial biogenesis and oxidative metabolism and myosin heavy chain (2, 6, 31). In addition, these molecules are thought to mediate exercise-induced transient or chronic metabolic responses.Because some aging-related increases in obesity, lifestylerelated diseases, and physical impairment are caused by a lack of exercise and irregular eating habits, regular exercise and dietary improvements are recommended for their prevention (24, 36). Thus, many attempts have been made to maintain/ improve physical performance and to suppress lifestyle-related diseases by nutritional and physical means (41, 42); however, their efficacy and practicality are limited and remain controversial (12, 17).Green tea, one of the most popula...

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Section: Body Weight and Tissue Weightsmentioning

confidence: 99%

Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice

Murase¹,

Haramizu²,

Ota³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Muscle tissue was homogenized and extracted for total RNA using the acid guanidium thiocyanate-phenol-chloroform extraction method (Chomcyznski & Sacchi 1987) and modified according to methods described elsewhere (Febbraio & Koukoulas 2000). RNA samples were reverse transcribed using a thermal cycler (Perkin Elmer GeneAmp PCR 2400 thermal cycler; Perkin Elmer, Rowville, Victoria, Australia) with Taqman Reverse transcription reagents (Applied Biosystems, Foster City, CA, USA) in 40 µl reaction mixtures containing 1× Taqman RT buffer, 5·5 mM MgCl 2 , 500 µM 2 -deoxynucleoside 5 -triphosphate, 2·5 µM random hexamers, 0·4U/µl RNase inhibitor and 1·25 U/µl multiscribe reverse transcriptase.…”

Section: Muscle Analysismentioning

confidence: 99%

Suppression of plasma free fatty acids upregulates peroxisome proliferator-activated receptor (PPAR) α and δ and PPAR coactivator 1α in human skeletal muscle, but not lipid regulatory genes

Watt

Southgate

Holmes

et al. 2004

Journal of Molecular Endocrinology

132

103

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Fatty acids are an important ligand for peroxisome proliferator-activated receptor (PPAR) activation and transcriptional regulation of metabolic genes. To examine whether reduced plasma free fatty acid (FFA) availability affects the mRNA content of proteins involved in fuel metabolism in vivo, the skeletal muscle mRNA content of various transcription factors, transcriptional coactivators and genes encoding for lipid regulatory proteins were examined before and after 3 h of cycle exercise with (NA) and without (CON) pre-exercise ingestion of nicotinic acid (NA). NA resulted in a marked (3-to 6-fold) increase (P,0·05) in PPAR , PPAR and PPAR coactivator 1 (PGC1 ) mRNA, but was without effect on nuclear respiratory factor-1 and Forkhead transcription factor, fatty acid transcolase/CD36, carnitine palmitoyl transferase 1, hormone sensitive lipase (HSL) and pyruvate dehydrogenase kinase 4. Exercise in CON was associated with increased (P,0·05) PPAR , PPAR and PGC1 mRNA, which was similar in magnitude to levels observed with NA at rest. Exercise was generally without effect on the mRNA content of lipid regulatory proteins in CON and did not affect the mRNA content of the measured subset of transcription factors, transcriptional co-activators and lipid regulatory proteins during NA. To determine the possible mechanisms by which NA might affect PGC1 expression, we measured p38 MAP kinase (MAPK) and plasma epinephrine. Phosphorylation of p38 MAPK was increased (P,0·05) by NA treatment at rest, and this correlated (r 2 =0·84, P,0·01) with increased PGC1 . Despite this close relationship, increasing p38 MAPK in human primary myotubes was without effect on PGC1 mRNA content. Plasma epinephrine was elevated (P,0·05) by NA at rest (CON: 0·27±0·06, NA: 0·72±0·11 nM) and throughout exercise. Incubating human primary myotubes with epinephrine increased PGC1 independently of changes in p38 MAPK phosphorylation. Hence, despite the fact that NA ingestion decreased FFA availability, it promoted the induction of PPAR / and PGC1 gene expression to a similar degree as prolonged exercise. We suggest that the increase in PGC1 may be due to the elevated plasma epinephrine levels. Despite these changes in transcription factors/coactivators, the mRNA content of lipid regulatory proteins was generally unaffected by plasma FFA availability.

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“…Determination of HSL mRNA by RT-PCR Approximately 60 mg of adipose was extracted for total RNA using a modification of the acid guanidium thiocyanate-phenol chloroform extraction method described elsewhere [22]. The total RNA was quantified and 1 ng of each total RNA sample was reverse-transcribed in a 10-ml reaction previously described [22].…”

Section: Determination Of Hsl Protein By Western Blot Analysismentioning

confidence: 99%

“…The total RNA was quantified and 1 ng of each total RNA sample was reverse-transcribed in a 10-ml reaction previously described [22]. Control samples were also analysed where all the above reagents are added to RNA samples except the Multiscribe Reverse Transcriptase.…”

Section: Determination Of Hsl Protein By Western Blot Analysismentioning

confidence: 99%

Hormone-sensitive lipase is reduced in the adipose tissue of patients with type 2 diabetes mellitus: influence of IL-6 infusion

et al. 2004

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Aims/hypothesis: Type 2 diabetes mellitus is characterised by increased plasma NEFA and IL-6 concentrations, and IL-6 increases lipolysis in healthy men. We assessed the adipose tissue hormone-sensitive lipase (HSL) mRNA expression, protein expression and HSL activity in patients with type 2 diabetes mellitus, and determined the effect of IL-6 administration on these measures. Methods: Seven patients with type 2 diabetes mellitus (age 67±4 years, weight 87±7 kg) and six age-and weight-matched individuals visited the laboratory on two occasions. Subcutaneous adipose tissue biopsies and blood samples were obtained prior to and during 3 h of either saline or recombinant human IL-6 infusion. Results: HSL mRNA was reduced (p<0.05) by ∼40% in type 2 diabetes mellitus relative to control subjects, while HSL protein expression showed a tendency to be decreased (35%, p=0.09). HSL activity averaged 8.87±1.25 and 6.91±1.20 nmol min −1 mg −1 protein for control and type 2 diabetic subjects respectively (p<0.05). IL-6 administration increased (p<0.05) HSL mRNA 2-fold at 60 min in both groups, whereas HSL protein and activity were unaffected by IL-6. Plasma insulin was elevated (p<0.05) in patients with type 2 diabetes mellitus at rest and was blunted (p<0.05) during IL-6 infusion in both groups. Plasma glucagon and cortisol were elevated (p<0.05) by IL-6 in both groups. Conclusions/interpretation: Our data demonstrate that basal HSL is decreased in patients with type 2 diabetes mellitus, and this may be a consequence of elevated plasma insulin levels. We have also shown that IL-6 administration increases HSL gene expression, although it exerted no effect on HSL protein and activity. This disparity between mRNA, protein and enzyme activity may be a function either of the marked alterations in the hormonal milieu induced by IL-6 administration and/or of post-transcriptional events.

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HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise

Cited by 148 publications

References 28 publications

Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice

Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice

Suppression of plasma free fatty acids upregulates peroxisome proliferator-activated receptor (PPAR) α and δ and PPAR coactivator 1α in human skeletal muscle, but not lipid regulatory genes

Hormone-sensitive lipase is reduced in the adipose tissue of patients with type 2 diabetes mellitus: influence of IL-6 infusion

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