Hsp70 expression in shrimp Litopenaeus vannamei in response to IHHNV and WSSV infection

Valentim-Neto, Pedro Alexandre; Moser, Juliana Righetto; Fraga, Ana Paula M.; Marques, Maria Risoleta Freire

doi:10.1007/s13337-014-0236-6

Cited by 24 publications

(11 citation statements)

References 23 publications

(22 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ding, Wang, Sun, Guo and Dong (2009) shrimp. Virus infection promoted changes in the expression of Hsp70 in Litopenaeus vannamei (Valentim-Neto, Moser, Fraga, & Marques, 2014). Transcription of LvHSP70 was induced in haemocytes and hepatopancreas of Litopenaeus vannamei after different bacteria injection (Zhou, Wang, He, Zheng, Wang, Xin, Liu, & Wang, 2009).…”

Section: Discussionmentioning

confidence: 99%

Untitled

Gao¹,

He²,

vector³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Untitled

Gao¹,

He²,

vector³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

“…Western blot analysis in the gill tissue of L . vannamei indicated that the expression of HSP70 was up-regulated by infecting the pathogens of white spot syndrome (WSSV) and hypodermal-hematopoietic necrosis (IHHNV) [127]. The mRNA and protein expression levels of HSP70 were observed highest in the heat-stress group of goats ( Capra hircus ) [128].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

Huang

Cheng

et al. 2018

PLoS ONE

View full text Add to dashboard Cite

Soil salinization erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 ± 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), anti-lipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins “Polymerase (RNA) II (DNA directed) polypeptide A” (POLR2A), “Histone acetyltransferase p300” (EP300) and “Heat shock 70kDa protein 8” (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins “Heat shock 70kDa protein 4” (HSPA4), “FBJ murine osteosarcoma viral oncogene homolog” (FOS) and “Nucleoporin 54kDa” (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of “EP300-HSPA8” and “HSPA4-NUP54” were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.

show abstract

“…A heat shock protein 70 (Hsc70) was found to inhibit apoptosis induced by WSSV infection in hemocyte shrimp cells [51]. In shrimp P. vannamei, the expression of the Hsp70 gene was also reported altered after the WSSV infection [52,53], and intramuscularly injection of Hsp70 protein could significantly reduce mortality after WSSV infection [54]. As for the Hsp40 gene, its responses to viral infection have been reported in halibut Paralichthys olivaceus [55].…”

Section: Nimav-1_lva Genes Involved In Host-pathogen Interactionmentioning

confidence: 99%

The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei

Bao

Tang

Alcivar–Warren³

2020

Genes

View full text Add to dashboard Cite

White spot syndrome virus (WSSV), the lone virus of the genus Whispovirus under the family Nimaviridae, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other closely related free-living viruses for comparative studies. In this study, we reconstructed a full-length endogenous nimavirus consensus genome, Nimav-1_LVa (279,905 bp), in the genome sequence of Penaeus (Litopenaeus) vannamei breed Kehai No. 1 (ASM378908v1). This endogenous virus seemed to insert exclusively into the telomeric pentanucleotide microsatellite (TAACC/GGTTA)n. It encoded 117 putative genes, with some containing introns, such as g012 (inhibitor of apoptosis, IAP), g046 (crustacean hyperglycemic hormone, CHH), g155 (innexin), g158 (Bax inhibitor 1 like). More than a dozen Nimav-1_LVa genes are involved in the pathogen-host interactions. We hypothesized that g046, g155, g158, and g227 (semaphorin 1A like) were recruited host genes for their roles in immune regulation. Sequence analysis indicated that a total of 43 WSSV genes belonged to the ancestral/core nimavirus gene set, including four genes reported in this study: wsv112 (dUTPase), wsv206, wsv226, and wsv308 (nucleocapsid protein). The availability of the Nimav-1_LVa sequence would help understand the genetic diversity, epidemiology, evolution, and virulence of WSSV.

show abstract

Hsp70 expression in shrimp Litopenaeus vannamei in response to IHHNV and WSSV infection

Cited by 24 publications

References 23 publications

Untitled

Untitled

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei

Contact Info

Product

Resources

About