hsp70 AS New Cadmium Bioaccumulation Marker to prevent the risks of Mussels Consumption in Human Nutrition

Piscopo, Marina; Notariale, Rosaria; Troisi, Jacopo

doi:10.18689/mjcr-1000107

Cited by 5 publications

(3 citation statements)

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“…These results add insight to the inducibility of Mytilus galloprovincialis stress genes by cadmium, also at non-lethal levels. In fact, we have recently reported (Piscopo et al, 2017) the occurrence of gill hsp70 response to cadmium in the same range of concentrations used as in this work. Indeed, a positive correlation was observed between cadmium concentration in gills of Mytilus galloprovincialis and hsp70 expression.…”

Section: Icp-ms Analyses Of Cadmium Bioaccumulation In Testes Of Mytisupporting

confidence: 56%

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

et al. 2018

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Here we report the industrial pollution effects due to cadmium on the reproductive health of Mytilus galloprovincialis. Mussels were removed from the biofouling of a Conatex panel after one year exposition at a polluted site near a disposal metallurgical factory. A high cadmium bioaccumulation was observed in the testis of mussels housed at the polluted site, with respect to a control site, as determined by inductively coupled plasma-mass spectrometry, along with a 10 fold increase in metallothionein 20 kDa gene (mt20) expression levels determined by qPCR. Furthermore, mussels transferred into laboratory tanks from the reference site, and exposed to 1.5, 5 and 10 µM CdCl, revealed a 1.7, 3.2 and 4.5 fold expression increase in the testis mt20, respectively, and a positive correlation with cadmium bioaccumulation was found. To evaluate a potential detrimental risk of such alterations on spermatozoa, we carried out electrophoretic analyses on their protamine-like proteins. As determined by AU-PAGE, after 1.5 µM CdCl exposure, protamine-like proteins also display major alterations with respect to those obtained after 5 and 10 µM CdCl exposure. All protamine-like proteins isolated from the polluted biofouling were in an aggregated form and displayed the same reduced DNA binding affinity of the protamine-like proteins obtained after 1.5 µM CdCl as demonstrated EMSA with sperm genomic DNA. Our results contribute to the studies concerning cadmium induced testis alterations and highlight protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on Mytilus galloprovincialis, for the sensitivity of the in vivo and in vitro changes of protamine-like proteins' state and their DNA binding affinity.

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Section: Icp-ms Analyses Of Cadmium Bioaccumulation In Testes Of Mytisupporting

confidence: 56%

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

et al. 2018

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“…Further, we have recently proposed the PL proteins both as antibacterial agents (Notariale et al, ) and as an emerging biomarker for cadmium impact assessment on male M . galloprovincialis (De Guglielmo et al, ; Piscopo, Notariale, et al, ; Piscopo, Notariale, & Troisi, ; Piscopo, Trifuoggi, Notariale, et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…They represent the 76% of the overall nuclear protein in M. galloprovincialis with PL-III (11.3 kDa) present in the highest amount (50%) and PL-II (14 kDa), and PL-IV (6.5 kDa), which represent the 20% and 6%, respectively (Vassalli et al, 2015). Further, we have recently proposed the PL proteins both as antibacterial agents and as an emerging biomarker for cadmium impact assessment on male M. galloprovincialis (De Guglielmo et al, 2019;Piscopo, Notariale, & Troisi, 2017;Piscopo, Trifuoggi, Notariale, et al, 2018).…”

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confidence: 99%

Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Lettieri

Maione

Ranauda

et al. 2019

Molecular Reproduction Devel

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Salinity represents a critical environmental and an ecological factor in the reproduction of marine species. As global climate changes and anthropogenic factors affect salinity, in this study, we have analyzed the responses of Mytilus galloprovincialis spermatozoa to hyposaline stress. We exposed mussels, in laboratory tanks, for 24 hr at 18°C to control (35.9 psu) and three hyposaline (17.1, 22.6, and 26.2 psu) conditions, and evaluated the expression of sperm hsp70 and protamine‐like proteins genes. Further we analyzed the electrophoretic pattern, the DNA binding and the release from sperm nuclei of protamine‐like proteins. For all experimental approaches used, the results obtained at 17.1 psu condition were very similar to those obtained in the control condition, while alterations were always recorded at 22.6 and 26.2 psu conditions. Particularly, at 22.6 and 26.2 psu, was observed: 42.5‐ and 17.1‐fold increase in hsp70 expression, respectively, and hypoexpression of PL‐II/PLIV protamine‐like proteins genes. Further, electrophoretic mobility shift assays and salt‐induced release of nuclear proteins from sperm nuclei, revealed alterations in the PL proteins/DNA binding, in these two hyposaline conditions. The similarity between the results obtained in control and in the more severe hyposaline condition (17.1 psu) could indicate a phenomenon of fertility preservation strategy due to gamete plasticity.

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Pancreatic Antioxidative Defense and Heat Shock Proteins Prevent Islet of Langerhans Cell Death After Chronic Oral Exposure to Cadmium LOAEL Dose

Moroni-González,

Sarmiento-Ortega,

Diaz

et al. 2023

Biol Trace Elem Res

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hsp70 AS New Cadmium Bioaccumulation Marker to prevent the risks of Mussels Consumption in Human Nutrition

Cited by 5 publications

References 0 publications

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

Protamine-like proteins analyses as emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

Molecular effects on spermatozoa of Mytilus galloprovincialisexposed to hyposaline conditions

Pancreatic Antioxidative Defense and Heat Shock Proteins Prevent Islet of Langerhans Cell Death After Chronic Oral Exposure to Cadmium LOAEL Dose

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